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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01742
  • pan locus tag?: SAUPAN004239000
  • symbol: SAOUHSC_01742
  • pan gene symbol?: relA
  • synonym:
  • product: GTP pyrophosphokinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01742
  • symbol: SAOUHSC_01742
  • product: GTP pyrophosphokinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1644149..1646338
  • length: 2190
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACAACGAATATCCATATAGTGCAGACGAAGTTCTTCACAAAGCAAAATCATATTTG
    TCAGCAGATGAATATGAGTATGTTTTAAAAAGCTATCATATTGCTTATGAAGCACATAAA
    GGTCAGTTCCGAAAAAACGGATTACCATACATTATGCATCCTATACAAGTTGCAGGTATT
    TTAACAGAAATGCGATTAGACGGACCGACGATTGTCGCAGGTTTTTTGCATGATGTAATT
    GAAGATACACCGTATACATTTGAAGATGTAAAAGAAATGTTCAATGAAGAAGTTGCTCGA
    ATTGTTGATGGTGTGACGAAGCTTAAAAAAGTAAAATACCGCTCAAAAGAAGAACAACAA
    GCTGAAAATCATCGCAAGTTATTTATTGCGATTGCCAAAGATGTACGCGTAATTTTGGTG
    AAATTAGCAGACAGATTACATAATATGCGTACCTTGAAAGCCATGCCGCGCGAAAAACAA
    ATTAGAATTTCTCGAGAAACATTAGAAATTTATGCACCATTAGCACATCGTCTTGGTATT
    AATACAATCAAATGGGAACTAGAAGATACGGCTCTTCGTTATATTGATAATGTGCAATAT
    TTTAGAATAGTCAATTTAATGAAGAAGAAACGTAGTGAACGTGAAGCGTATATCGAAACG
    GCTATTGATAGAATACGTACTGAAATGGACCGAATGAATATCGAAGGCGATATAAATGGT
    AGACCTAAACATATTTACAGTATTTATCGGAAAATGATGAAGCAGAAAAAACAATTTGAT
    CAAATTTTTGATTTGTTGGCGATACGTGTTATTGTCAATTCTATTAATGATTGTTATGCG
    ATACTTGGGTTGGTGCATACGTTATGGAAACCGATGCCAGGACGTTTTAAAGATTATATT
    GCAATGCCTAAACAAAATTTGTATCAGTCATTGCATACTACAGTAGTAGGCCCAAATGGA
    GACCCGCTCGAAATCCAAATACGAACGTTTGATATGCACGAAATTGCTGAGCATGGTGTT
    GCAGCACACTGGGCTTACAAAGAAGGTAAAAAAGTAAGTGAAAAAGATCAAACTTATCAA
    AATAAGTTAAATTGGTTAAAAGAATTAGCTGAAGCGGATCATACATCGTCTGACGCTCAA
    GAATTTATGGAAACCTTAAAATATGACTTACAGAGTGACAAAGTATACGCATTTACCCCA
    GCGAGTGATGTTATTGAGTTGCCATATGGTGCTGTGCCGATTGATTTTGCTTATGCGATT
    CACAGTGAAGTAGGTAATAAGATGATTGGTGCCAAGGTGAATGGCAAAATTGTACCAATT
    GACTATATTTTACAAACAGGCGATATTGTTGAAATACGTACTAGTAAACATTCATATGGA
    CCAAGTCGTGATTGGTTGAAAATTGTTAAATCGTCTAGTGCCAAAGGTAAAATTAAAAGT
    TTCTTCAAAAAACAAGATCGTTCATCTAATATTGAAAAAGGCCGAATGATGGTTGAAGCT
    GAAATAAAAGAGCAAGGATTTAGAGTCGAAGATATTTTGACAGAGAAAAATATTCAGGTT
    GTTAATGAAAAATATAACTTTGCAAATGAAGATGATTTATTCGCAGCTGTAGGATTTGGC
    GGCGTGACATCCTTACAGATTGTTAATAAATTAACTGAAAGACAACGTATTTTAGATAAA
    CAACGTGCTTTAAATGAAGCACAAGAAGTTACGAAATCATTGCCTATTAAAGACAACATC
    ATTACTGATAGTGGTGTCTATGTAGAAGGTTTAGAAAATGTACTTATCAAGTTGTCAAAA
    TGTTGTAATCCTATACCAGGTGATGATATTGTAGGTTATATCACCAAAGGTCACGGTATT
    AAAGTACATCGCACTGATTGCCCAAATATTAAGAACGAAACTGAACGACTAATTAATGTT
    GAATGGGTAAAATCAAAAGACGCAACTCAAAAATATCAGGTTGATTTAGAGGTAACTGCG
    TATGACCGAAATGGCTTGTTGAATGAAGTACTACAAGCTGTTAGCTCGACAGCCGGCAAT
    TTAATTAAAGTTTCAGGACGTTCAGATATTGATAAAAATGCAATAATAAATATTAGTGTC
    ATGGTGAAAAACGTGAATGATGTTTATCGTGTGGTAGAAAAGATCAAACAACTTGGTGAT
    GTTTATACAGTAACAAGAGTTTGGAACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2190

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01742
  • symbol: SAOUHSC_01742
  • description: GTP pyrophosphokinase
  • length: 729
  • theoretical pI: 8.59224
  • theoretical MW: 83693.5
  • GRAVY: -0.438683

Function[edit source | edit]

  • reaction:
    EC 2.7.6.5?  ExPASy
    GTP diphosphokinaseATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate
  • TIGRFAM:
    Cellular processesCellular processesAdaptations to atypical conditionsRelA/SpoT family protein (TIGR00691; HMM-score: 802.9)
    TIGR04552 family protein (TIGR04552; HMM-score: 15.8)
    Unknown functionGeneralTIGR04562 family protein (TIGR04562; HMM-score: 14.9)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairnon-canonical purine NTP pyrophosphatase, RdgB/HAM1 family (TIGR00042; EC 3.6.1.-; HMM-score: 13.1)
  • TheSEED:  
    CBSS-176299.4.peg.1292 Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) / GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II 
    Plastidial (p)ppGpp-mediated response in plants Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) / GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II 
    Stringent Response, (p)ppGpp metabolism Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) / GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II 
  • PFAM:
    HD_PDEase (CL0237) HD_4; HD domain (PF13328; HMM-score: 160.3)
    NTP_transf (CL0260) RelA_SpoT; Region found in RelA / SpoT proteins (PF04607; HMM-score: 136.5)
    Ubiquitin (CL0072) TGS; TGS domain (PF02824; HMM-score: 72.1)
    ACT (CL0070) ACT_4; ACT domain (PF13291; HMM-score: 67.3)
    HD_PDEase (CL0237) HD; HD domain (PF01966; HMM-score: 46.1)
    ACT (CL0070) ACT; ACT domain (PF01842; HMM-score: 30.2)
    Ubiquitin (CL0072) Ub-Mut7C; Mut7-C ubiquitin (PF14451; HMM-score: 14.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SAOUHSC_02492(rplO)50S ribosomal protein L15  [2] (data from MRSA252)
    SAOUHSC_02477(rpsI)30S ribosomal protein S9  [2] (data from MRSA252)
    SAOUHSC_00529elongation factor G  [2] (data from MRSA252)
    SAOUHSC_00871D-alanine--poly(phosphoribitol) ligase subunit 2  [2] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.099
    • Ymax_pos: 38
    • Cmax: 0.122
    • Cmax_pos: 23
    • Smax: 0.138
    • Smax_pos: 3
    • Smean: 0.077
    • D: 0.09
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFRKNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIVDGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQIRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYIETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSINDCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHEIAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQSDKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIVEIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFRVEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNEAQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHRTDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLIKVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. 2.0 2.1 2.2 2.3 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  3. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  5. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]