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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01744
  • pan locus tag?: SAUPAN004241000
  • symbol: SAOUHSC_01744
  • pan gene symbol?: recJ
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01744
  • symbol: SAOUHSC_01744
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1647306..1649579
  • length: 2274
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATTAAACCGAAGTATAAGTGGAAATTAACTAAACCAGCTGAATACATAAGTGATGAA
    TTAACATCAAAATTAAAGTTAACGCCAATCGTAAAAAAAATATTAGAAAGTAAATCGATT
    ATTGATGAACAAGCAATAGAATCCATCATTTCAGATACAGATATCAATCATGATGCACTG
    CAATTGAGCGATATGACTAAGACTATTGAAAGAATTAAAAGAGCGATAGCGAATGATGAG
    AAAATCCTAGTTTATGGTGATTACGATGCCGACGGTGTTACCGCAACTACAATTTTAGTG
    ATAACATTACAACTTTTAGGTGCACAAGTAGGATGGCATATTCCTAACCGCTTTACCGAA
    GGATATGGACCAAACGAACTAGCATTTAGAAATGCTCATGATGAAGGCATAACATTAATC
    ATAACTGTTGATAATGGCATACAAGGTCATAATGAAATTAAAATGGTTCAAGATTTAGGA
    GTTGATGTCATTGTAACTGATCATCATGAAATTGGTAGTACATTACCAGAAGCATATGCA
    ATCGTACATCCAATGCATCCATCGTTTAATTATCCGTTCCAACAATTGTGTGGTGCAGGT
    GTTGCATACAAATTAGCACAAGCATTGATTGAAAATGTACCTGATTATTTTAAAGCATTA
    GTTGCGATAGGTACCATTGCGGATTTAGTATCCTTAACTGATGAAAATCGATCATTAGTG
    AAACAAGGATTAAAAGTCTTAAATGATCAATGTCCGACCTCTGTTAAAGCATTGCTAAAA
    GAAGCGGGATACAATGACAATATTGATGAAGAAACAATTGGTTTTATTATTGGACCTAGA
    CTTAATGCTGTAGGTCGTTTAGATGACGCATCATTAGCTTGCGAATTGTTGATGACTGAC
    GTCGAAGAAGAAGCTGCATTTTTAGCAGAACAAGTTGAACATTTTAACCGTGAAAGAAAA
    GATATTGTAGCAACTATCACAGAAGAAGCAATGGCTATGGCAGAAACGAAAGTTAAAAAG
    GGAGATTTATTTTTACTTTTAGCAAAAGAAAATTGGCATGAAGGTGTCTTAGGTATTGTT
    GCTTCGAAAATTGTCGAAACATTTGCTTTGCCAACATTAATTTTAAATATTGATAGGGAG
    CAAAATCATGCAAAAGGCTCCGCCAGATCAATTGATCAAGTGTCGATGTTTGAAATACTA
    TCAGCACATCAAGAGTTAATCGCTAAATTTGGCGGCCATCATATGGCTGCAGGTATGACG
    ATGGATATTGAAAATATAGAATCGTTAGCTGAAGGTTTAAATAAATGGATGAAAGAATTA
    TCTAAAACAACTTCGTTAGATCCCGTAAAGCAAGTAGATGTATTATTAACAGAAAATGAT
    ATAACGATTAAAAATATTCGTGATATGAATCGTTTAAGACCCTTCGGAACAGATTTTTCT
    AGACCTATTTTTGAAATGGATGATTTATCAGTTTCTTCAGTTAAGGCGATAGGTCAACAA
    AAAAATCATCTAAAATTGACACTTGGTGAAAGTAATATCGCCGCGCTATTTTGGCAAAAT
    GGACACTTAGAACCTGAGTTACAAGATGAACAGCCAATTAATATATTAGGATCTGTTCAA
    ATCAACGAATGGAATGGTAATCAATCACCGCAAATAATTATTCAAGATATTGCGATGAAT
    GAACAGCAAATATTAGATTATAGAAGTAAGCGAAAAAGTTTACCTTTTACAGAAAATGAT
    GAAAATATTGTCGTGCTTATTCATCCTAAAAGTGATAAAGTAAATGCGAATGAATATTAT
    TATGGTGAAGAAATTAAACAACAAACTGATAAAGTAGTATTAAGAGATTTACCAACGTCA
    ATGGAAGACTTGTCTAATTCCTTGCAACAACTGCAATTTTCTCAACTTTATATAGTTTTG
    CAACATAATCATTCGATTTACTTCGATGGTATACCTAATATGGATATTTTTAAAAAGTGT
    TATAAAGCATTAATAACTAAACAAGAAACAAATATCCAGAAAGAGGGTATGTTATTGTGT
    CAACATTTAAGTGTGAAACCAGATACACTTAAATTCATGTTGAAAGTTTTCTTAGACTTA
    AAATTTGTAACACAAGAAGATGGTTTAATTCGAATCAATCAACAACCTGATAAAAGATCG
    ATTGATTCCAGCAAAGTATATCAATTAAGACAACAACGTATGGATGTTGAAAAGCAATTA
    TTATATCAAGATTTTTCAGAAATAAAAAATTGGATAAAGTCACAATTGTCGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2274

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01744
  • symbol: SAOUHSC_01744
  • description: hypothetical protein
  • length: 757
  • theoretical pI: 4.90644
  • theoretical MW: 85610.4
  • GRAVY: -0.269485

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairsingle-stranded-DNA-specific exonuclease RecJ (TIGR00644; EC 3.1.-.-; HMM-score: 635.6)
  • TheSEED:  
    DNA MetabolismDNA repairDNA Repair Base Excision Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-) 
    DNA repairDNA repair, bacterial RecFOR pathway Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-) 
    DNA replicationDNA-replication Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-) 
  • PFAM:
    no clan definedssDNA-exonuc_C; Single-strand DNA-specific exonuclease, C terminal domain (PF10141; HMM-score: 195.9)
    DHHA1; DHHA1 domain (PF02272; HMM-score: 76.8)
    HAD (CL0137) DHH; DHH family (PF01368; HMM-score: 46.4)
    Rep (CL0169) DUF1424; Putative rep protein (DUF1424) (PF07232; HMM-score: 10.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.129
    • Ymax_pos: 46
    • Cmax: 0.114
    • Cmax_pos: 46
    • Smax: 0.229
    • Smax_pos: 1
    • Smean: 0.111
    • D: 0.122
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQAIESIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTATTILVITLQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLGVDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFKALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFIIGPRLNAVGRLDDASLACELLMTDVEEEAAFLAEQVEHFNRERKDIVATITEEAMAMAETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSIDQVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVKQVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTLGESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMNEQQILDYRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDLSNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQHLSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQLLYQDFSEIKNWIKSQLS

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]