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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01827
  • pan locus tag?: SAUPAN004370000
  • symbol: SAOUHSC_01827
  • pan gene symbol?: ezrA
  • synonym:
  • product: septation ring formation regulator EzrA

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01827
  • symbol: SAOUHSC_01827
  • product: septation ring formation regulator EzrA
  • replicon: chromosome
  • strand: -
  • coordinates: 1732038..1733732
  • length: 1695
  • essential: yes [1] DEG other strains
  • comment: The sequence of SAOUHSC_01827 was corrected based on the resequencing performed by Berscheid et al., 2012 [2].

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGTGTTATATATCATTTTGGCAATAATTGTGATTATATTGATTGCTGTAGGTGTATTA
    TTCTATTTACGTTCAAATAAACGACAAATTATTGAAAAAGCAATCGAACGTAAAAATGAA
    ATTGAGACGTTACCTTTTGATCAAAACCTTGCACAATTATCTAAGTTGAATTTAAAAGGT
    GAAACAAAAACGAAATACGATGCAATGAAAAAGGACAACGTAGAAAGTACAAATAAGTAT
    CTAGCTCCTGTGGAAGAAAAAATCCATAATGCTGAGGCTTTATTAGATAAATTTAGTTTC
    AACGCATCTCAAAGTGAAATTGATGATGCAAATGAGTTGATGGATAGTTACGAACAAAGC
    TATCAGCAACAATTAGAAGATGTAAATGAAATTATTGCGTTATACAAAGATAATGATGAA
    TTATATGACAAATGTAAGGTTGATTATCGTGAAATGAAACGTGATGTTTTAGCAAATCGT
    CATCAATTTGGTGAGGCAGCAAGTCTACTTGAAACTGAAATTGAAAAATTTGAGCCAAGG
    TTAGAGCAATATGAAGTACTAAAAGCTGATGGTAATTATGTACAAGCGCACAACCATATA
    GCTGCCTTGAATGAACAAATGAAACAGCTAAGATCTTATATGGAAGAAATACCAGAATTA
    ATTAGAGAAACTCAAAAAGAATTACCTGGTCAATTCCAAGATTTAAAATATGGTTGCCGT
    GATCTTAAAGTTGAAGGGTATGATCTGGATCACGTGAAAGTAGACAGTACATTACAAAGC
    TTAAAAACAGAGCTTAGTTTCGTTGAACCATTAATTAGCCGCTTAGAATTAGAAGAAGCT
    AATGATAAACTAGCTAATATCAATGATAAGTTAGATGACATGTATGATTTAATTGAACAT
    GAAGTTAAAGCTAAAAATGATGTCGAAGAAACAAAAGATATCATTACGGATAACTTATTC
    AAAGCTAAAGACATGAATTATACATTGCAAACAGAAATTGAATATGTACGTGAAAACTAC
    TATATAAATGAATCTGATGCTCAGAGTGTTCGTCAATTTGAAAATGAAATTCAAAGTTTA
    ATTTCTGTATATGATGATATTTTAAAAGAAATGTCTAAATCTGCTGTGCGATATAGCGAG
    GTTCAGGATAATTTACAATATTTAGAAGATCATGTCACAGTTATTAATGACAAACAAGAA
    AAGCTACAAAATCATCTGATTCAATTGCGTGAAGATGAAGCAGAAGCAGAAGACAATCTG
    CTACGAGTACAATCGAAGAAAGAAGAAGTGTATCGTCGATTACTTGCTTCTAACTTAACA
    AGCGTTCCTGAAAGGTTTATCATCATGAAAAATGAAATTGATCATGAAGTTCGTGATGTT
    AACGAACAATTTAGTGAACGTCCAATACACGTTAAACAGTTAAAAGATAAAGTGTCTAAA
    ATTGTGATTCAAATGAATACATTTGAAGATGAAGCAAATGATGTTCTTGTTAATGCTGTT
    TATGCAGAGAAATTAATTCAATATGGAAATAGATATCGTAAGGACTATAGCAATGTTGAT
    AAGAGCTTAAATGAAGCTGAACGATTATTTAAAAATAATCGCTATAAGCGTGCGATTGAA
    ATTGCAGAGCAAGCTCTTGAAAGTGTTGAGCCAGGTGTTACTAAACATATTGAAGAAGAA
    GTTATTAAGCAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1695

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01827
  • symbol: SAOUHSC_01827
  • description: septation ring formation regulator EzrA
  • length: 564
  • theoretical pI: 4.50272
  • theoretical MW: 66199
  • GRAVY: -0.686348

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesCell divisionchromosome segregation protein SMC (TIGR02168; HMM-score: 22.7)
    Genetic information processingDNA metabolismChromosome-associated proteinschromosome segregation protein SMC (TIGR02168; HMM-score: 22.7)
    Cellular processesCellular processesCell divisionchromosome segregation protein SMC (TIGR02169; HMM-score: 22.7)
    Genetic information processingDNA metabolismChromosome-associated proteinschromosome segregation protein SMC (TIGR02169; HMM-score: 22.7)
    Genetic information processingProtein fateProtein folding and stabilizationcytochrome c-type biogenesis protein CcmI (TIGR03142; HMM-score: 5.9)
    MetabolismEnergy metabolismElectron transportcytochrome c-type biogenesis protein CcmI (TIGR03142; HMM-score: 5.9)
  • TheSEED:  
    Cell Division and Cell CycleCell Division and Cell Cycle - no subcategoryControl of cell elongation - division cycle in Bacilli Septation ring formation regulator EzrA 
    CBSS-393130.3.peg.794 Septation ring formation regulator EzrA 
  • PFAM:
    no clan definedEzrA; Septation ring formation regulator, EzrA (PF06160; HMM-score: 529.1)
    PI3K_P85_iSH2; Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain (PF16454; HMM-score: 22.7)
    GPW_gp25; Gene 25-like lysozyme (PF04965; HMM-score: 18.1)
    Ac110_PIF; Per os infectivity factor AC110 (PF07280; HMM-score: 15.6)
    TPR (CL0020) TPR_2; Tetratricopeptide repeat (PF07719; HMM-score: 12.5)
    no clan definedASFV_J13L; African swine fever virus J13L protein (PF05568; HMM-score: 12.4)
    DUF2106; Predicted membrane protein (DUF2106) (PF09879; HMM-score: 12)
    TPR (CL0020) ANAPC3; Anaphase-promoting complex, cyclosome, subunit 3 (PF12895; HMM-score: 12)
    TPR_16; Tetratricopeptide repeat (PF13432; HMM-score: 11.1)
    no clan definedMed9; RNA polymerase II transcription mediator complex subunit 9 (PF07544; HMM-score: 10.8)
    IncA; IncA protein (PF04156; HMM-score: 9.3)
    SIT; SHP2-interacting transmembrane adaptor protein, SIT (PF15330; HMM-score: 9.2)
    HTH (CL0123) MerR-DNA-bind; MerR, DNA binding (PF09278; HMM-score: 7.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.158
    • Ymax_pos: 35
    • Cmax: 0.145
    • Cmax_pos: 35
    • Smax: 0.258
    • Smax_pos: 2
    • Smean: 0.137
    • D: 0.15
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETLPFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNASQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANRHQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIPELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLELEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIEYVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHVTVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMKNEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQYGNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAOUHSC_01827 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Anne Berscheid, Peter Sass, Konstantin Weber-Lassalle, Ambrose L Cheung, Gabriele Bierbaum
    Revisiting the genomes of the Staphylococcus aureus strains NCTC 8325 and RN4220.
    Int. J. Med. Microbiol.: 2012, 302(2);84-7
    [PubMed:22417616] [WorldCat.org] [DOI] (I p)
  3. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  5. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

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