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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01840
  • pan locus tag?: SAUPAN004390000
  • symbol: SAOUHSC_01840
  • pan gene symbol?: sgtA
  • synonym:
  • product: transglycosylase domain-containing protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01840
  • symbol: SAOUHSC_01840
  • product: transglycosylase domain-containing protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1746311..1747216
  • length: 906
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGACGAATCAAGACAACAATCATCAATTGAATCATCGTATATATCATTTTGAAAAGATA
    TATAAAGCTATCAAACATGTCATTGTTTTTATATTTATGATTTTCATTGCCATCGTTGCT
    ATCGCTGTGATTGCGATGTCTTTATATTTTCATCATTTAACTAAAACGTCCGACTCATTA
    TCAGATGATGCTTTAATAAAAAAAGTTCGACAAATACCTGGCGATGAATTATTAGATCAT
    AATAACAAAAATTTATTATATGAGTATAACCATTCTCAAAACTCACTCATTATAGGCCCT
    AAAACATCAAGTCCAAATGTCATTAAAGCATTAACGTCATCTGAAGACACTTTATTTTAT
    AAACATGATGGCATCTTACCAAAGGCGATTTTAAGAGCAATGATACAAGATATTTTTAAT
    ACTGATCAAAGTTCAGGTGGTAGCACAATTACACAACAACTTGTTAAAAATCAAGTTCTT
    ACCAACGAAAAAACATATAGTAGAAAAGCAAATGAACTTCGCCTAGCAATTAGATTAGAA
    CACCTACTCTCAAAAGATGAAATTATATATACATATTTAAATATAGTTCCCTTCGGTAGA
    GATTATAATGGCGCTAATATTTCCGGAATTGCATCCGCTTCATATAGTCTATTTGGTATT
    CCACCAAAAGATTTATCAATTGCACAATCTGCATACCTTATCGGTTTGTTGCAAAGCCCA
    TATGGCTATACACCCTACGAAAAAGATGGAACGTTAAAATCGGATAAAGATTTGAAATAT
    AGTATTCAAAGACAACATTATGTATTAAAGCGTATGTTAATCGAAGATCAAATCACTGAA
    AAAGAATACAACGACGCATTAAAATATGATATTAAATCACATTTGTTAAATCGAAAAAAG
    CGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    906

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01840
  • symbol: SAOUHSC_01840
  • description: transglycosylase domain-containing protein
  • length: 301
  • theoretical pI: 9.50571
  • theoretical MW: 34526.3
  • GRAVY: -0.341196

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanpenicillin-binding protein, 1A family (TIGR02074; HMM-score: 172.3)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanpenicillin-binding protein 1C (TIGR02073; HMM-score: 136.6)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanpenicillin-binding protein 1B (TIGR02071; HMM-score: 126.7)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanmonofunctional biosynthetic peptidoglycan transglycosylase (TIGR02070; EC 2.4.2.-; HMM-score: 96)
  • TheSEED:  
    Cell Wall and CapsuleCell Wall and Capsule - no subcategoryPeptidoglycan Biosynthesis Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) 
  • PFAM:
    Lysozyme (CL0037) Transgly; Transglycosylase (PF00912; HMM-score: 184.6)
    no clan definedPspB; Phage shock protein B (PF06667; HMM-score: 11.6)
    E-set (CL0159) Arch_flagellin; Archaebacterial flagellin (PF01917; HMM-score: 10.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 9.99
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 5
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.144
    • Ymax_pos: 31
    • Cmax: 0.162
    • Cmax_pos: 46
    • Smax: 0.295
    • Smax_pos: 24
    • Smean: 0.165
    • D: 0.152
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVIAMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKTSSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVLTNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLFGIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIEDQITEKEYNDALKYDIKSHLLNRKKR

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAOUHSC_01840 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]