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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02089
  • pan locus tag?: SAUPAN004876000
  • symbol: SAOUHSC_02089
  • pan gene symbol?: int2
  • synonym:
  • product: phage family integrase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02089
  • symbol: SAOUHSC_02089
  • product: phage family integrase
  • replicon: chromosome
  • strand: +
  • coordinates: 1965883..1966929
  • length: 1047
  • essential: no DEG

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGCCAGTATATAAGGATGATAATACAGGTAAATGGTATTTTTCCATTAGATATAAAGAT
    GTATACGGTAATAACAAACGAAAAATGAAGCGTGGGTTTGAACGTAAGAAAGATGCCAAA
    CTAGCTGAAAGCGAATTTATACAAAATGTTAAATATGGATACTCGGACAATCAACCCTTT
    GAATATATATTTTTTGATCGTTTAAAAAATGAAAATCTTTCTGCACGCTCAATAGAAAAG
    CGAACTACAGAATATAATACTCACATAAAAGAAAGGTTCGGAAATATCCCTATTGGCAAA
    ATCACTACTACGCAATGTACTGCTTTCAGGAATTATTTGTTAAACGATGCAGGTCTTTCT
    GTTGACTATGCACGATCTGTGTGGGCAGGTTTTAAAGCAGTTATCAATTACGCCAAAAAG
    CATTACAAGCTCTTATACGACCCCACATTATCGGTAACTCCTATTCCCAGAACAAAACCA
    CAAGCTAAATTTATCACTCGTGAAGAATTTGATGAAAAAGTAGAACAAATCACAAATGAT
    ACTTCTCGTCAGCTAACTAGACTGTTATTTTATTCTGGTCTTAGAATAGGAGAAGCTTTA
    GCTTTGCAGTGGAAAGATTACGATAAAATAAAAGGCGAAATTGACGTAAATAAGAAAATC
    AATTTAAGTAATAGAAAAATTGAATATAATCTAAAAAAAGAAAGCTCTAAAGGGATAATA
    CCTGTACCAAATTTAATTAGAGAGATGCTTAAAAACATGTATAATGAATCTTCTAAAAGA
    TATAAATATTTTGACGAAAACTATTTTATATTCGGGGGTTTAGAACCTATTAGATACGTT
    ACTTATTCGTATCATTTTAAATCTGTATTCCCGAATCTAAAAATACACCATTTAAGACAC
    TCGTACGCTAGCTATTTAATTAATAATGGTGTAGATATGTATTTATTAATGGAATTAATG
    AGGCATTCTAACATTACAGAAACAATTCAAACGTACTCTCATTTATATACTGATAAAAAA
    CATCAAGCTATGAGCATATTTGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1047

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02089
  • symbol: SAOUHSC_02089
  • description: phage family integrase
  • length: 348
  • theoretical pI: 9.94345
  • theoretical MW: 41378
  • GRAVY: -0.71523

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairtyrosine recombinase XerC (TIGR02224; HMM-score: 51.9)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairtyrosine recombinase XerD (TIGR02225; HMM-score: 45.4)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairintegron integrase (TIGR02249; HMM-score: 44.4)
    Genetic information processingMobile and extrachromosomal element functionsOtherintegron integrase (TIGR02249; HMM-score: 44.4)
  • TheSEED:  
    Phage integrase 
  • PFAM:
    DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 68)
    MBD-like (CL0081) Arm-DNA-bind_4; Arm DNA-binding domain (PF14657; HMM-score: 45.8)
    l-integrase_N (CL0469) Phage_int_SAM_3; Phage integrase, N-terminal SAM-like domain (PF14659; HMM-score: 30.5)
    Phage_int_SAM_5; Phage integrase SAM-like domain (PF13102; HMM-score: 26.9)
    no clan definedRib_recp_KP_reg; Ribosome receptor lysine/proline rich region (PF05104; HMM-score: 11.6)
    DUF5126; Domain of unknown function (DUF5126) (PF17166; HMM-score: 10.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.114
    • Ymax_pos: 41
    • Cmax: 0.106
    • Cmax_pos: 6
    • Smax: 0.175
    • Smax_pos: 29
    • Smean: 0.098
    • D: 0.107
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MPVYKDDNTGKWYFSIRYKDVYGNNKRKMKRGFERKKDAKLAESEFIQNVKYGYSDNQPFEYIFFDRLKNENLSARSIEKRTTEYNTHIKERFGNIPIGKITTTQCTAFRNYLLNDAGLSVDYARSVWAGFKAVINYAKKHYKLLYDPTLSVTPIPRTKPQAKFITREEFDEKVEQITNDTSRQLTRLLFYSGLRIGEALALQWKDYDKIKGEIDVNKKINLSNRKIEYNLKKESSKGIIPVPNLIREMLKNMYNESSKRYKYFDENYFIFGGLEPIRYVTYSYHFKSVFPNLKIHHLRHSYASYLINNGVDMYLLMELMRHSNITETIQTYSHLYTDKKHQAMSIFD

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SAOUHSC_00515 (SigH*) [3]
    SigH* (sigma factor) controls competence and phage integrase genes
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Liang Tao, Xiaoqian Wu, Baolin Sun
    Alternative sigma factor sigmaH modulates prophage integration and excision in Staphylococcus aureus.
    PLoS Pathog.: 2010, 6(5);e1000888
    [PubMed:20485515] [WorldCat.org] [DOI] (I e)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]