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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02312
  • pan locus tag?: SAUPAN005349000
  • symbol: SAOUHSC_02312
  • pan gene symbol?: kdpA
  • synonym:
  • product: potassium-transporting ATPase subunit A

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02312
  • symbol: SAOUHSC_02312
  • product: potassium-transporting ATPase subunit A
  • replicon: chromosome
  • strand: -
  • coordinates: 2142498..2144174
  • length: 1677
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGAAATAATTTTGTTTCTAACAATGATGGTTATGATTACGTATGTTTTTAGTGGATAT
    CTATATCGCGTAGCATTGGTGCAATCGTCAAGAGTAGATTTAATATTTACTAGATTTGAA
    AATATGTGTTTTAAAATTATCGGCACAGATTTAGAACACATGTCAGCTAAAACATATGTC
    AAACATTTTCTGGCATTTAATGGATTTATGGGGTTTATAACATTTGTATTGCTAATAGTT
    CAGCAATGGCTTTTTTTAAATCCAAATCATAATTTAAACCAATCGATAGATTTAGCGTTT
    AATACAGCAATATCTTTTTTAACAAATAGTAATTTACAACACTATAACGGTGAATCAGAT
    GTGACATATTTAACGCAAATGATTGTAATGACATATTTAATGTTTACATCTAGTGCATCA
    GGTTACGCCGTTTGTATAGCGATGTTGAGACGTTTAACTGGATTAACTAATATCATTGGT
    AATTTTTATCAAGATATTGTTCGGTTTATTGTCCGAGTACTTTTACCATTATCATGTTTA
    ATTAGTATTTTATTGATGACTCAAGGTGTACCACAAACGTTGCATGCTAATTTAATGATT
    CGGACTTTAAGCGGACATATTCAACATATTGCATTTGGACCTATTGCATCACTTGAATCA
    ATAAAACATCTTGGTACGAATGGTGGAGGATTTTTAGCAGGAAATTCTGCAACACCTTTT
    GAAAATCCAAATATTTGGAGCAATTTTATAGAAATGGGCAGTATGATGTTACTTCCTATG
    TCAATGTTGTTCTTATTTGGTCGCATGTTAAGTAGACATGGTAAACGAGTACATCGTCAT
    GCGTTGATATTATTTGTCGCAATGTTTTTCATTTTTATAGCAATTCTTACATTAACTATG
    TGGAGTGAGTATCGTGGTAATCCAATACTAGCGAATTTAGGCATTTATGGACCGAATATG
    GAAGGTAAAGAGGTACGGTTTGGAGCAGGTTTGTCAGCACTATTTACAGTTATTACGACG
    GCATTTACAACGGGTTCTGTTAATAACATGCATGATAGCTTAACGCCTATAGGTGGATTA
    GGACCAATGGTATTAATGATGCTAAATGTTGTATTTGGTGGCGAAGGCGTAGGACTCATG
    AATTTATTGATATTTGTCTTACTGACGGTGTTTATATGCAGTTTGATGGTTGGTAAAACA
    CCAGAATATTTAAATATGCCAATTGGCGCCCGTGAAATGAAATGTATTGTCTTAGTCTTT
    CTCATACACCCAATTTTAATTTTAGTATTTTCAGCACTTGCTTTTATGATTCCTGGAGCA
    AGTGAAAGTATAACGAATCCGTCTTTTCATGGTATTTCACAAGTTATGTATGAAATGACA
    TCAGCTGCTGCGAACAATGGATCAGGGTTTGAAGGACTGAAAGATGATACAACATTCTGG
    AATATCTCTACAGGAATCATTATGTTGCTTTCTCGTTATATACCAATTATTTTGCAATTA
    ATGATTGCATCAAGTTTAGTGAATAAAAAATCATACCATCAAGATAAATATACAATAGCG
    ATTGATAAACCTTATTTTGGCGTATCACTAATCGTATTTATCGTTTTACTAAGTGGTTTG
    ACATTTATTCCAGTACTATTACTTGGTCCTATTGGTGAATTTTTAACTTTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1677

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02312
  • symbol: SAOUHSC_02312
  • description: potassium-transporting ATPase subunit A
  • length: 558
  • theoretical pI: 8.44178
  • theoretical MW: 62025.5
  • GRAVY: 0.688351

Function[edit source | edit]

  • reaction:
    EC 3.6.3.12?  ExPASy
    Potassium-transporting ATPaseATP + H2O + K+(Out) = ADP + phosphate + K+(In)
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsK+-transporting ATPase, A subunit (TIGR00680; EC 3.6.3.12; HMM-score: 580.7)
  • TheSEED:  
    Potassium metabolismPotassium metabolism - no subcategoryPotassium homeostasis Potassium-transporting ATPase A chain (EC 3.6.3.12) (TC 3.A.3.7.1) 
  • PFAM:
    Ion_channel (CL0030) KdpA; Potassium-transporting ATPase A subunit (PF03814; HMM-score: 706.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.118
    • Ymax_pos: 11
    • Cmax: 0.12
    • Cmax_pos: 57
    • Smax: 0.218
    • Smax_pos: 1
    • Smean: 0.122
    • D: 0.12
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCFKIIGTDLEHMSAKTYVKHFLAFNGFMGFITFVLLIVQQWLFLNPNHNLNQSIDLAFNTAISFLTNSNLQHYNGESDVTYLTQMIVMTYLMFTSSASGYAVCIAMLRRLTGLTNIIGNFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASLESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKRVHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSALFTVITTAFTTGSVNNMHDSLTPIGGLGPMVLMMLNVVFGGEGVGLMNLLIFVLLTVFICSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILILVFSALAFMIPGASESITNPSFHGISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVNKKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]