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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02327
  • pan locus tag?: SAUPAN005371000
  • symbol: SAOUHSC_02327
  • pan gene symbol?: yidC
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02327
  • symbol: SAOUHSC_02327
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2158348..2159220
  • length: 873
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAAGAAAAAAGCGTTACTACCATTATTTTTAGGTATTATGGTCTTTTTGGCTGGTTGT
    GACTATTCTAAACCTGAAAAACGTAGTGGGTTTTTCTACAATACATTCGTAGATCCAATG
    AAAAATGTATTGGATTGGTTGGGAAATAACTTATTAAACGACAATTATGGTTTAGCTATT
    ATTATCCTTGTATTGGTAATTCGTATTATTTTATTACCATTCATGTTGTCAAACTATAAA
    AATAGTCATATGATGCGTCAAAAAATGAAAGTTGCAAAGCCAGAAGTTGAAAAAATTCAA
    GAAAAAGTGAAACGTGCGCGTACACAAGAAGAAAAAATGGCTGCAAACCAAGAATTAATG
    CAAGTATATAAAAAGTATGACATGAACCCGATTAAGAGTATGTTGGGTTGTTTACCAATG
    CTAATCCAATTACCAATCATCATGGGATTATACTTTGTACTTAAAGACCAACTTGTAGAT
    GGTTTGTTTAAATATCCACACTTCTTATGGTTCGATTTAGGACGTCCTGATATTTGGATT
    ACAATTATTGCCGGTGTTTTATACTTTATCCAAGCATATGTATCAAGTAAAACGATGCCA
    GACGAACAACGTCAAATGGGTTACATGATGATGGTCATTTCACCAATTATGATTATCTGG
    ATTTCATTAAGCTCAGCATCAGCACTTGGTTTGTACTGGTCAGTCAGTGCGGCGTTCCTT
    GTAGTTCAAACACACTTTGCGAACATTTATTATGAAAAAGTCGCTAAAAAAGAAGTACAA
    CCTTTCATTGAAGCGTATGAAAGAGAGCACAACGGCGGCAGCAATAAAAAAGGCAAAAAC
    ACACAAGTTGTGTCTAAAAAGAAAAAGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    873

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02327
  • symbol: SAOUHSC_02327
  • description: hypothetical protein
  • length: 290
  • theoretical pI: 10.1207
  • theoretical MW: 33580.9
  • GRAVY: 0.0365517

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein fateProtein and peptide secretion and traffickingmembrane protein insertase, YidC/Oxa1 family (TIGR03592; HMM-score: 160.7)
  • TheSEED:  
    Cell Division Subsystem including YidCD Inner membrane protein translocase and chaperone YidC, short form OxaI-like 
  • PFAM:
    Oxa1 (CL0376) 60KD_IMP; 60Kd inner membrane protein (PF02096; HMM-score: 151.5)
    no clan definedEI24; Etoposide-induced protein 2.4 (EI24) (PF07264; HMM-score: 20.2)
    DUF4181; Domain of unknown function (DUF4181) (PF13789; HMM-score: 13.9)
    Succ_DH_flav_C; Fumarate reductase flavoprotein C-term (PF02910; HMM-score: 13.8)
    MLANA; Protein melan-A (PF14991; HMM-score: 13.8)
    MFS (CL0015) OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 11.6)
    no clan definedDUF4446; Protein of unknown function (DUF4446) (PF14584; HMM-score: 11.5)
    Trigger_C (CL0262) SurA_N_2; SurA N-terminal domain (PF13623; HMM-score: 10.7)
    Hypoth_1 (CL0447) DUF3784; Domain of unknown function (DUF3784) (PF12650; HMM-score: 8.9)
    no clan definedDUF5325; Family of unknown function (DUF5325) (PF17259; HMM-score: 8.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane(Lipid modified N-termini)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPII)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: VFLAGCD
  • SignalP: no predicted signal peptide
    • Ymax: 0.237
    • Ymax_pos: 11
    • Cmax: 0.126
    • Cmax_pos: 29
    • Smax: 0.661
    • Smax_pos: 1
    • Smean: 0.548
    • D: 0.358
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVLDWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEKVKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVDGLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMIIWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNKKGKNTQVVSKKKKK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]