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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40

NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02472
  • pan locus tag?: SAUPAN005646000
  • symbol: SAOUHSC_02472
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02472
  • symbol: SAOUHSC_02472
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2295453..2296589
  • length: 1137
  • essential: no DEG

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGGAAATTCATGTTTATACGGGACCGCTTTGTGGATTTAATGAATATATTAATTCTGAA
    GTTAAAGAATATCACCGCTTCATGGAATTAATCAGAGAATACAATATCATAGTGCGAGCA
    AGTGATACATTTGGAACTCATGATGTTGACGGTATTTTTGATACTGAGATAGAAAATCTT
    GTGATATTTTCAAACGACTTTGCTTCTGTAACAACTCATGTTATTACTAATTTTATAAAT
    ATCGTTACTTTAGGTAGAAATATTAAGAAAATCTATATTCAGAATCCTCCAAAAAGAGTA
    TTTCAATCACTAAATAGTTTTCACGATTCTGAAATAATAGAAATAAACTATGAATATACA
    ACATTAAAAAAAGATGATGTAATAAATTTTTATAAAGAGCAGATGGCTGGTTCTAAAATA
    ATAGGTCAAACAGACGCTAAAAAACAAATGAGCATAGATTTATATAAACATACTTCCTTA
    GAAAATAATAAACCGACCGTAATGCTTTACTATGGTCCATCAGGTGTTGGAAAAACAGAA
    TTAGCCAAAGAAATAGCAAAAAAATATAAAGGTAATATCACTAGAATACAATTTTCTATG
    ATGCAGAATGATGAATCTTTTAATTATATCTTTGGAGATGAACATGCCAAAGTATCATTG
    TCAAAAGATTTAGTAGATCGTGAAACTAATGTTGTTTTAATTGATGAATTTGATAAGGTA
    TTACCAATCTATTACAATGTTTTCTATCAAATGTTTGATGAAGGTATTATTGAAGATGCC
    AATTATAAAGTAGACGTTTCAAATTGTATTTTCATTCTAACATCTAATTTTAATAGTATT
    GAAGAAGCAATAGTTAATATTGGAGAACCGGTATTTTCTAGAATAGATTCCTGTATTAAA
    TTTTTATATTTAACACAGGAAGAAAAGCAGAAAGTAATAGAAAATAAATTAAATGAAATA
    CTATCATCTCTAAATGAAAAAGAAAAAAGAATAGTTACCGCATATAACTTGTTAGAGAAA
    TATAAAGAGACAGAAATAGATATCGATAACATTAGGTATTTAAAGAAAATAATAACAAAT
    GATATTTATACAATAATTTTTGAAGATGAATTATTTAATACGGATATTATAGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1137

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02472
  • symbol: SAOUHSC_02472
  • description: hypothetical protein
  • length: 378
  • theoretical pI: 4.58881
  • theoretical MW: 44099.8
  • GRAVY: -0.289947

Function[edit source | edit]

  • TIGRFAM:
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesATP-dependent Clp protease ATP-binding subunit ClpA (TIGR02639; HMM-score: 67.3)
    Cellular processesCellular processesPathogenesistype VI secretion ATPase, ClpV1 family (TIGR03345; HMM-score: 61.1)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype VI secretion ATPase, ClpV1 family (TIGR03345; HMM-score: 61.1)
    Genetic information processingProtein fateProtein folding and stabilizationATP-dependent chaperone protein ClpB (TIGR03346; HMM-score: 57)
    and 12 more
    Genetic information processingDNA metabolismDNA replication, recombination, and repairHolliday junction DNA helicase RuvB (TIGR00635; EC 3.6.4.12; HMM-score: 33.1)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesendopeptidase La (TIGR00763; EC 3.4.21.53; HMM-score: 24.8)
    Genetic information processingProtein fateProtein folding and stabilizationATP-dependent protease HslVU, ATPase subunit (TIGR00390; HMM-score: 23.6)
    Cellular processesCellular processesOthergas vesicle protein GvpN (TIGR02640; HMM-score: 19.1)
    26S proteasome subunit P45 family (TIGR01242; HMM-score: 18.3)
    AAA family ATPase, CDC48 subfamily (TIGR01243; HMM-score: 17.2)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairorc1/cdc6 family replication initiation protein (TIGR02928; HMM-score: 15.6)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesproteasome ATPase (TIGR03689; EC 3.6.4.8; HMM-score: 15)
    Cellular processesCellular processesChemotaxis and motilityflagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 13.4)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingsignal recognition particle-docking protein FtsY (TIGR00064; HMM-score: 12.8)
    phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 12.5)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype VII secretion AAA-ATPase EccA (TIGR03922; HMM-score: 11)
  • TheSEED:  
    Protein MetabolismProtein degradationProteolysis in bacteria, ATP-dependent ClpB protein 
    and 1 more
    Protein foldingProtein chaperones ClpB protein 
  • PFAM:
    P-loop_NTPase (CL0023) AAA_2; AAA domain (Cdc48 subfamily) (PF07724; HMM-score: 56.2)
    and 22 more
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 44.5)
    AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 27.6)
    RuvB_N; Holliday junction DNA helicase ruvB N-terminus (PF05496; HMM-score: 25.7)
    AAA_3; ATPase family associated with various cellular activities (AAA) (PF07726; HMM-score: 24.4)
    AAA_14; AAA domain (PF13173; HMM-score: 22.2)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 19.8)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 19.5)
    AAA_22; AAA domain (PF13401; HMM-score: 19.2)
    AAA_18; AAA domain (PF13238; HMM-score: 19.1)
    NB-ARC; NB-ARC domain (PF00931; HMM-score: 15.7)
    AAA_24; AAA domain (PF13479; HMM-score: 15)
    ABC_tran; ABC transporter (PF00005; HMM-score: 14.9)
    IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 14.6)
    AAA_17; AAA domain (PF13207; HMM-score: 14.2)
    TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 14)
    Zeta_toxin; Zeta toxin (PF06414; HMM-score: 13.5)
    AAA_33; AAA domain (PF13671; HMM-score: 13.1)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 13)
    no clan definedDUF4795; Domain of unknown function (DUF4795) (PF16043; HMM-score: 12.8)
    P-loop_NTPase (CL0023) AAA_19; AAA domain (PF13245; HMM-score: 12.5)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 12.2)
    AAA_25; AAA domain (PF13481; HMM-score: 12.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.093
    • Ymax_pos: 22
    • Cmax: 0.128
    • Cmax_pos: 22
    • Smax: 0.114
    • Smax_pos: 19
    • Smean: 0.061
    • D: 0.08
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MEIHVYTGPLCGFNEYINSEVKEYHRFMELIREYNIIVRASDTFGTHDVDGIFDTEIENLVIFSNDFASVTTHVITNFINIVTLGRNIKKIYIQNPPKRVFQSLNSFHDSEIIEINYEYTTLKKDDVINFYKEQMAGSKIIGQTDAKKQMSIDLYKHTSLENNKPTVMLYYGPSGVGKTELAKEIAKKYKGNITRIQFSMMQNDESFNYIFGDEHAKVSLSKDLVDRETNVVLIDEFDKVLPIYYNVFYQMFDEGIIEDANYKVDVSNCIFILTSNFNSIEEAIVNIGEPVFSRIDSCIKFLYLTQEEKQKVIENKLNEILSSLNEKEKRIVTAYNLLEKYKETEIDIDNIRYLKKIITNDIYTIIFEDELFNTDIID

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]