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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02573
  • pan locus tag?: SAUPAN005769000
  • symbol: SAOUHSC_02573
  • pan gene symbol?: nhaC
  • synonym:
  • product: Na+/H+ antiporter NhaC

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02573
  • symbol: SAOUHSC_02573
  • product: Na+/H+ antiporter NhaC
  • replicon: chromosome
  • strand: -
  • coordinates: 2363234..2364634
  • length: 1401
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGATGAAAAGAAAACCCACCTTTTTAGAATCAATTTCGACAATGATTGTAATGGTTATT
    GTTGTTGTAACAGGCTTTGTGTTTTTTGATATTCCAATTCAAGTATTATTAATTATTGCC
    TCAGCATATGCCACATGGATTGCAAAACGTGTAGGCTTAACATGGCAAGATTTAGAAAAA
    GGCATTGCAGAACGTTTAAATACTGCAATGCCTGCAATTTTAATTATACTAGCGGTAGGA
    ATTATAGTAGGCAGTTGGATGTTTTCTGGCACAGTGCCAGCCTTGATTTATTATGGCTTA
    GATTTATTGAATCCAAGCTATTTTTTAATATCAGCCTTTTTTATAAGTGCTGTTACATCT
    GTAGCAACTGGTACAGCATGGGGCTCTGCATCAACTGCAGGGATTGCACTTATTTCTATT
    GGTAATCAATTGGGGATTCCTCCAGGGATGGCAGCGGGTGCTATTATAGCAGGGGCTGTG
    TTTGGCGATAAAATGTCACCATTATCAGATACAACTAATTTAGCGGCGCTTGTTACTAAA
    GTTAATATATTTAAACATATACATTCGATGATGTGGACGACGATACCTGCATCAATCATA
    GGTTTATTAGTATGGTTTATTGCTGGATTTCAATTTAAAGGGCATTCAAATGATAAACAG
    ATTCAAACTTTGTTATCAGAGCTTGCACAGATTTATCAAATTAACATATGGGTCTGGGTT
    CCCTTAATTGTGATCATTGTTTGTTTGCTATTTAAAATGGCTACAGTGCCAGCTATGCTA
    ATATCAAGCTTTTCTGCCATTATAGTGGGGACTTTTAATCATCATTTCAAAATGACAGAT
    GGTTTCAAAGCAACATTTAGTGGTTTTAACGAATCAATGATACATCAGTCTCATATTTCA
    TCCAGTGTGAAAAGCTTGTTAGAACAGGGTGGTATGATGAGTATGACCCAAATATTAGTA
    ACGATATTTTGCGGATATGCATTTGCAGGTATTGTAGAAAAAGCAGGATGTTTAGAAGTC
    TTATTAACTACTATTTCTAAAGGCATCCATTCTGTAGGAAGTTTAATATGTATTACTGTT
    ATTTGTTGTATTGCGCTTGTATTCGCTGCAGGTGTTGCTTCGATTGTAATTATTATGGTC
    GGTGTGTTAATGAAAGATTTGTTCGAAAAATACCAAGTCTCTCGTTCCGTTTTATCAAGA
    ACACTTGAAGACTCAAGTACGATGGTCTTACCTTTAATACCATGGGGAACATCAGGTATT
    TACTATACGAATCAACTTCATGTCTCTGTTGAAGAATTTTTCATATGGACAGTACCATGT
    TATTTATGCGCAATTATAGCAATTATCTATGGTTTTACAGGGATAGGTATTAAAAAGTCA
    TCGAATTCACGTTTAACTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1401

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02573
  • symbol: SAOUHSC_02573
  • description: Na+/H+ antiporter NhaC
  • length: 466
  • theoretical pI: 8.38218
  • theoretical MW: 50436.9
  • GRAVY: 0.886266

Function[edit source | edit]

  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsNa+/H+ antiporter NhaC (TIGR00931; HMM-score: 434.4)
  • TheSEED:  
    Na+/H+ antiporter NhaC 
  • PFAM:
    IT (CL0182) Na_H_antiporter; Na+/H+ antiporter family (PF03553; HMM-score: 242.1)
    and 1 more
    no clan definedVpu; Vpu protein (PF00558; HMM-score: 8.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.238
    • Ymax_pos: 13
    • Cmax: 0.144
    • Cmax_pos: 30
    • Smax: 0.64
    • Smax_pos: 6
    • Smean: 0.563
    • D: 0.365
  • predicted transmembrane helices (TMHMM): 10

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MMKRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYATWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDLLNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISIGNQLGIPPGMAAGAIIAGAVFGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSNDKQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHFKMTDGFKATFSGFNESMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEKAGCLEVLLTTISKGIHSVGSLICITVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQVSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVEEFFIWTVPCYLCAIIAIIYGFTGIGIKKSSNSRLT

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SAOUHSC_02298 regulon (SigB*) [1] other strains
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]