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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02632
  • pan locus tag?: SAUPAN005858000
  • symbol: SAOUHSC_02632
  • pan gene symbol?: tcaB
  • synonym:
  • product:

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02632
  • symbol: SAOUHSC_02632
  • product:
  • replicon: chromosome
  • strand: -
  • coordinates: 2420344..2421551
  • length: 1209
  • essential: no DEG other strains
  • comment: The sequence of SAOUHSC_02632 was corrected based on the resequencing performed by Berscheid et al., 2012 [1].

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATGATTTAACGCATTTTAACAAGCGTTCACCGATATTTATTATTATTTTAGGATCT
    TTGACGGCCATTGGAGCATTATCGATAGATATGTTTTTGCCTGGATTACCAGATATTAGA
    CATGATTTTCAAACAACGACGTCTAACGCACAATTAACATTATCGATGTTTATGATTGGT
    TTAGCGTTTGGTAATTTGTTTGCTGGTCCAATTTCAGATTCAACTGGGCGCAGAAAGCCG
    CTTATTATAGCTATGATTATTTTTACATTAGCAAGTTTAGGTATTGTTTTTGTACATAAT
    ATTTGGCTGATGGTTGCATTAAGATTTCTTCAAGGAGTGACAGGTGGCGCAGCAGCAGTT
    ATTTCAAGAGCTATAGCAAGTGATATGTATAGTGGTAATGAACTAACTAAGTTTATGGCA
    TTATTGATGCTTGTTAATGGTATTGCACCAGTTGTTGCGCCAACAATAGGGGGCATAATT
    TTAAATTATTCTGTCTGGCGTATGGTCTTTGTCATTCTGACTATCTTTGGCTTCGTCATG
    GTCATAGGTTCTTTATTAAAAGTACCTGAATCATTAACAGTGACAAATCGTGAGTCAAGT
    AGTGGATTAAAGACAATGTTTAAAAACTTCAAAATATTATTGAAGACGCCACGTTTTGTA
    TTACCAATGCTCATTCAAGGTATGACATTTGTAATATTATTTACTTATATTTCTGCGTCT
    CCGTTTATTATTCAAAAAATTTATGGCATGACTGCAATACAATTTAGTTGGATGTTTGCA
    GGAATTGGTATTACGCTCATTATATCAAGTCAACTTACTGGGTACCTTGTTGATTTTATA
    GACTCACAAAAATTGATGAGAGGCATGACGATGATTCAGATTATCGGTGTAATTTTAGTG
    ACGATTGTATTGCTTAATCATTGGAACTTTTGGATTTTAGCGATAGGATTTATCATTTTA
    ATAGCACCAGTGACAGGTGTTGCGACACTTGGTTTTACAATAGCTATGGATGAAAGTAGT
    AGTGGTAGAGGGAGTTCATCAAGTTTATTAGGTTTAGTACAGTTTTTATTTGGTGGTGTT
    GCATCGCCATTAGTGGGGGTAAAAGGTGAAGATAATCCTATACCTTACATAATTATTATT
    ATAGCTACAGCAGTTATATTAATTATTTTACAGATTTATAATATGAAGGTATTTAAAACA
    AATCGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1209

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02632
  • symbol: SAOUHSC_02632
  • description:
  • length: 402
  • theoretical pI: 10.4555
  • theoretical MW: 43822.5
  • GRAVY: 0.935572

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 289.4)
    and 14 more
    multidrug resistance protein (TIGR00880; HMM-score: 92.5)
    Cellular processesCellular processesToxin production and resistancedrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 69)
    MetabolismTransport and binding proteinsOtherdrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 69)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 68.8)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsH+ Antiporter protein (TIGR00900; HMM-score: 58.6)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsD-galactonate transporter (TIGR00893; HMM-score: 57.5)
    MetabolismTransport and binding proteinsCations and iron carrying compoundscation transport protein (TIGR00898; HMM-score: 45.9)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsphosphoglycerate transporter family protein (TIGR00881; HMM-score: 39.5)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, sugar porter (SP) family (TIGR00879; HMM-score: 30.6)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 30.4)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsoxalate/formate antiporter family transporter (TIGR00890; HMM-score: 25.8)
    MetabolismTransport and binding proteinsUnknown substrateMFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 24.5)
    MetabolismTransport and binding proteinsAnionsnitrite transporter (TIGR00886; HMM-score: 24.4)
    synaptic vesicle protein SV2 (TIGR01299; HMM-score: 23.9)
  • TheSEED:  
    Virulence, Disease and DefenseResistance to antibiotics and toxic compoundsTeicoplanin-resistance in Staphylococcus Teicoplanin resistance associated membrane protein TcaB 
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 149.4)
    and 3 more
    Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 50.6)
    MFS_4; Uncharacterised MFS-type transporter YbfB (PF06779; HMM-score: 35.3)
    MFS_3; Transmembrane secretion effector (PF05977; HMM-score: 34)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.173
    • Ymax_pos: 29
    • Cmax: 0.146
    • Cmax_pos: 29
    • Smax: 0.33
    • Smax_pos: 12
    • Smean: 0.24
    • D: 0.199
  • predicted transmembrane helices (TMHMM): 11

Accession numbers[edit source | edit]

  • GI:
  • RefSeq:
  • UniProt: Q2G2I5 UniProt

Protein sequence[edit source | edit]

  • MNDLTHFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQTTTSNAQLTLSMFMIGLAFGNLFAGPISDSTGRRKPLIIAMIIFTLASLGIVFVHNIWLMVALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGIILNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPRFVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYLVDFIDSQKLMRGMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTIAMDESSSGRGSSSSLLGLVQFLFGGVASPLVGVKGEDNPIPYIIIIIATAVILIILQIYNMKVFKTNR

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Anne Berscheid, Peter Sass, Konstantin Weber-Lassalle, Ambrose L Cheung, Gabriele Bierbaum
    Revisiting the genomes of the Staphylococcus aureus strains NCTC 8325 and RN4220.
    Int. J. Med. Microbiol.: 2012, 302(2);84-7
    [PubMed:22417616] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

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