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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02663
  • pan locus tag?: SAUPAN005899000
  • symbol: SAOUHSC_02663
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02663
  • symbol: SAOUHSC_02663
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2446555..2446938
  • length: 384
  • essential: no DEG other strains
  • comment: The sequence of SAOUHSC_02663 was corrected based on the resequencing performed by Berscheid et al., 2012 [1].

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    ATGAAAGAACGTGGCTATATCGAACAGCTTTGGCGTGAGGAAAAATATCATGTATTGTTA
    CACAGTCAACAGTCTTACCAAATGATAAGGAATGCTTTAAAAACGGATTTATCACTTCAT
    CAAGTTCAACAAATGATTGATGTTGCTTTGTTAATTGAACCGAGTATAGGAAGTGTATGT
    AATGCTTTTGATCATATGTGGGGATATTTAAAAAAATGTGCGAATGAAGAAGAAAGACAA
    CAATCAAAACTACTTAAAGCTGATTTTATTAATGGTAAAATCGACACCCAAACTTTATTA
    GATTTTTTAGCAGAACTTGCTAATAAATATGATGTTCAATATTTACTACAAAGTCGTGTT
    TTAAACACAAAAAGAAAGAGATAG
    60
    120
    180
    240
    300
    360
    384

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02663
  • symbol: SAOUHSC_02663
  • description: hypothetical protein
  • length: 127
  • theoretical pI: 8.66866
  • theoretical MW: 15044.2
  • GRAVY: -0.540945

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Probable type II DNA modification enzyme 
  • PFAM:
    no clan definedDUF1722; Protein of unknown function (DUF1722) (PF08349; HMM-score: 77.6)
    RecG_N; RecG N-terminal helical domain (PF17190; HMM-score: 14.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.106
    • Ymax_pos: 49
    • Cmax: 0.116
    • Cmax_pos: 63
    • Smax: 0.14
    • Smax_pos: 44
    • Smean: 0.074
    • D: 0.094
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKERGYIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQMIDVALLIEPSIGSVCNAFDHMWGYLKKCANEEERQQSKLLKADFINGKIDTQTLLDFLAELANKYDVQYLLQSRVLNTKRKR

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Anne Berscheid, Peter Sass, Konstantin Weber-Lassalle, Ambrose L Cheung, Gabriele Bierbaum
    Revisiting the genomes of the Staphylococcus aureus strains NCTC 8325 and RN4220.
    Int. J. Med. Microbiol.: 2012, 302(2);84-7
    [PubMed:22417616] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]