From AureoWiki
Jump to: navigation, search

NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02800
  • pan locus tag?: SAUPAN006110000
  • symbol: SAOUHSC_02800
  • pan gene symbol?: sarU
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02800
  • symbol: SAOUHSC_02800
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2575879..2576622
  • length: 744
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    GTGGACTATCAAACTTTCGAAAAGGTCAATAAATTCATAAATGTAGAAGCGTACATATTT
    TTTCTCACTCAAGAGTTAAAGCAACAATATAAATTATCATTAAAAGAATTGTTGATATTA
    GCATATTTTTATTACAAAAATGAACACAGTATTTCACTAAAAGAAATCATTGGTGACATA
    CTTTACAAACAATCTGATGTTGTAAAGAACATTAAGTCACTATCTAAAAAAGGATTTATA
    AATAAGTCTAGAAACGAAGCAGATGAACGCCGTATTTTTGTTTCAGTTACTCCAATACAA
    CGTAAAAAGATTGCTTGTGTTATTAATGAGTTAGATAAAATAATTAAAGGATTTAATAAG
    GAAAGAGACTACATAAAATATCAATGGGCTCCAAAATATAGCAAAGAATTTTTTATACTT
    TTTATGAACATTATGTACTCAAAAGATTTTTTAAAATATCGATTTAATTTAACATTTCTT
    GATTTATCTATCTTATATGTAATATCATCTCGAAAAAATGAGATACTAAATTTAAAAGAT
    TTGTTTGAAAGTATTAGATTTATGTATCCTCAAATTGTTAGGTCAGTTAATAGATTAAAT
    AATAAAGGTATGCTAATCAAAGAACGATCCCTTGCAGATGAAAGGATTGTGTTAATCAAA
    ATAAATAAAATACAATATAACACTATTAAAAGCATATTCACAGATACTTCCAAGATTCTC
    AAACCAAGAAAATTTTTCTTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    744

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02800
  • symbol: SAOUHSC_02800
  • description: hypothetical protein
  • length: 247
  • theoretical pI: 10.3367
  • theoretical MW: 29792.9
  • GRAVY: -0.245344

Function[edit source | edit]

  • TIGRFAM:
    Signal transductionRegulatory functionsDNA interactionsstaphylococcal accessory regulator family (TIGR01889; HMM-score: 210.6)
    and 1 more
    homoprotocatechuate degradation operon regulator, HpaR (TIGR02337; HMM-score: 35.4)
  • TheSEED:  
    Transcriptional regulator SarU (Staphylococcal accessory regulator U) 
  • PFAM:
    HTH (CL0123) MarR; MarR family (PF01047; HMM-score: 36.3)
    HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 34.9)
    MarR_2; MarR family (PF12802; HMM-score: 29.8)
    and 4 more
    TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 19.3)
    Rrf2; Transcriptional regulator (PF02082; HMM-score: 14.2)
    HTH_Tnp_4; Helix-turn-helix of DDE superfamily endonuclease (PF13613; HMM-score: 14.1)
    OB (CL0021) CSD; 'Cold-shock' DNA-binding domain (PF00313; HMM-score: 12.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.094
    • Ymax_pos: 70
    • Cmax: 0.109
    • Cmax_pos: 29
    • Smax: 0.113
    • Smax_pos: 69
    • Smean: 0.06
    • D: 0.081
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFYYKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRKKIACVINELDKIIKGFNKERDYIKYQWAPKYSKEFFILFMNIMYSKDFLKYRFNLTFLDLSILYVISSRKNEILNLKDLFESIRFMYPQIVRSVNRLNNKGMLIKERSLADERIVLIKINKIQYNTIKSIFTDTSKILKPRKFFF

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAOUHSC_02800 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Adhar C Manna, Ambrose L Cheung
sarU, a sarA homolog, is repressed by SarT and regulates virulence genes in Staphylococcus aureus.
Infect. Immun.: 2003, 71(1);343-53
[PubMed:12496184] [WorldCat.org] (P p)