From AureoWiki
Jump to: navigation, search
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02852
  • pan locus tag?: SAUPAN006199000
  • symbol: SAOUHSC_02852
  • pan gene symbol?: cidR
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02852
  • symbol: SAOUHSC_02852
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2626357..2627184
  • length: 828
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACTAATGCTTCCAAATCATTATATATTGCACAACCTACAATTAGTAAAGCAATTAAA
    GATATTGAAAATGAAATGGGCACGCCCTTATTTGATAGAAGTAAAAGACATTTAATTCTT
    ACCGATGCAGGTCAAATTTTTTATGAGAAAAGTAAAGAAATTGTTGCACTGTATGATTAT
    TTACCATCTGAAATGGAACGCTTGAATGGACTGGAAACAGGACATATAAACATGGGCATG
    TCGGCAGTCATGAATATGAAGATTCTTATCAATATTCTTGGTGCATTCCATCAACAATAT
    CCAAATGTTACATATAATTTGATAGAAAATGGCGGTAAAACAATTGAACAGCAAATTATC
    AATGATGAAGTAGATATAGGCGTGACCACTTTGCCAGTCGATCATCATATTTTCGATTAT
    ACTACCCTAGATAAGGAAGATTTGCGACTTATCGTGAGCAGAGAGCATCGACTCGCAAAA
    TATGAAACTGTTAAACTCGAAGATTTAGCAGGTGAAGACTTCATTTTATTTAATAAAGAC
    TTTTACTTGAATGATAAAATTATTGAAAATGCTAAAAACGTTGGCTTTGTTCCGAATACT
    GTAGCGCAAATTTCACAATGGCATGTTATAGAAGATTTAGTTACGAATGAATTAGGTATT
    AGTATTTTACCAACATCAATTTCAGAGCAACTAAATGGAGATGTGAAGCTGCTACGCATT
    GAAGATGCTCATGTACATTGGGAATTAGGTGTTGTTTGGAAGAAGGATAAACAATTAAGT
    CATGCCACAACGAAATGGATAGAATTTTTGAAAGACCGTTTAGGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    828

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02852
  • symbol: SAOUHSC_02852
  • description: hypothetical protein
  • length: 275
  • theoretical pI: 5.07078
  • theoretical MW: 31465.8
  • GRAVY: -0.238182

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Signal transductionRegulatory functionsDNA interactionsaminoethylphosphonate catabolism associated LysR family transcriptional regulator (TIGR03339; HMM-score: 118.7)
    MetabolismEnergy metabolismOtherpca operon transcription factor PcaQ (TIGR02424; HMM-score: 69.9)
    Signal transductionRegulatory functionsDNA interactionspca operon transcription factor PcaQ (TIGR02424; HMM-score: 69.9)
    putative choline sulfate-utilization transcription factor (TIGR03418; HMM-score: 44.9)
    Signal transductionRegulatory functionsDNA interactionsD-serine deaminase transcriptional activator (TIGR02036; HMM-score: 32.9)
    Cellular processesCellular processesToxin production and resistancetranscriptional regulator, ArgP family (TIGR03298; HMM-score: 31)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairtranscriptional regulator, ArgP family (TIGR03298; HMM-score: 31)
    Signal transductionRegulatory functionsDNA interactionstranscriptional regulator, ArgP family (TIGR03298; HMM-score: 31)
    MetabolismFatty acid and phospholipid metabolismBiosynthesispoly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit (TIGR01836; HMM-score: 15.2)
  • TheSEED:  
    Regulation and Cell signalingProgrammed Cell Death and Toxin-antitoxin SystemsMurein hydrolase regulation and cell death LysR family regulatory protein CidR 
  • PFAM:
    PBP (CL0177) LysR_substrate; LysR substrate binding domain (PF03466; HMM-score: 144)
    HTH (CL0123) HTH_1; Bacterial regulatory helix-turn-helix protein, lysR family (PF00126; HMM-score: 49.3)
    no clan definedERp29; Endoplasmic reticulum protein ERp29, C-terminal domain (PF07749; HMM-score: 13.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.169
    • Ymax_pos: 19
    • Cmax: 0.206
    • Cmax_pos: 19
    • Smax: 0.252
    • Smax_pos: 18
    • Smean: 0.149
    • D: 0.161
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTNASKSLYIAQPTISKAIKDIENEMGTPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMNMKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTLDKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTVAQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQLSHATTKWIEFLKDRLG

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]