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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02921
  • pan locus tag?: SAUPAN006295000
  • symbol: SAOUHSC_02921
  • pan gene symbol?: budA2
  • synonym:
  • product: alpha-acetolactate decarboxylase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02921
  • symbol: SAOUHSC_02921
  • product: alpha-acetolactate decarboxylase
  • replicon: chromosome
  • strand: -
  • coordinates: 2685364..2686068
  • length: 705
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGACGAATGTCTTGTATCAACATGGTACATTAGGTACGTTAATGGCTGGCTTACTAGAA
    GGCACAGCTACAATTAATGAATTGTTAGAACATGGGAATTTAGGGATTGCAACGTTAACA
    GGGTCTGATGGCGAAGTAATATTTTTAGACGGAAAGGCATATCATGCTAACGAGCATAAA
    GAATTTATAGAATTAAAAGGCGATGAGAAAGTACCGTATGCATCGATTACTAATTTTAAA
    GCGAGTAAGACATTTCCATTGCAACAATTATCACAAGATGATGTATTTGCACAAATTAAA
    AATGAAATGTTAAGTGAGAATTTATTTTCGGCTGTTAAAATTTATGGCACATTTAAACAT
    ATGCATGTACGAATGATGCCTGCTCAGCAACCGCCATATACACGTTTGATTGATTCAGCA
    CGCAGACAACCTGAGGAAAAAAGACAAGATATTCGTGGTGCCATTGTTGGATTTTTTACA
    CCAGAATTATTTCATGGCGTAGGGTCTGCTGGTTTTCATATACATTTTGCGGATGATGAA
    AGAGCTTATGGTGGACATGTTCTTGACTTTGAAGTGGATGACGTTGTCGTTGAGATACAA
    AACTTTGAAACATTCCAACAACATTTCCCGGTAAATAACGAGACGTTTGTTAAAGCGAAA
    ATAGACTATAAAGATGTGGCAGAAGAAATTAGAGAAGCTGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    705

Protein[edit source | edit]

Protein Data Bank: 1XV2

General[edit source | edit]

  • locus tag: SAOUHSC_02921
  • symbol: SAOUHSC_02921
  • description: alpha-acetolactate decarboxylase
  • length: 234
  • theoretical pI: 4.90649
  • theoretical MW: 26406.5
  • GRAVY: -0.342735

Function[edit source | edit]

  • reaction:
    EC 4.1.1.5?  ExPASy
    Acetolactate decarboxylase(2S)-2-hydroxy-2-methyl-3-oxobutanoate = (3R)-3-hydroxybutan-2-one + CO2
  • TIGRFAM:
    MetabolismEnergy metabolismFermentationalpha-acetolactate decarboxylase (TIGR01252; EC 4.1.1.5; HMM-score: 245)
  • TheSEED:  
    CarbohydratesFermentationAcetoin, butanediol metabolism Alpha-acetolactate decarboxylase (EC 4.1.1.5) 
    Organic acidsAlpha-acetolactate operon Alpha-acetolactate decarboxylase (EC 4.1.1.5) 
    Regulation and Cell signalingProgrammed Cell Death and Toxin-antitoxin SystemsMurein hydrolase regulation and cell death Alpha-acetolactate decarboxylase (EC 4.1.1.5) 
  • PFAM:
    ALDC (CL0615) AAL_decarboxy; Alpha-acetolactate decarboxylase (PF03306; HMM-score: 249.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.141
    • Ymax_pos: 29
    • Cmax: 0.134
    • Cmax_pos: 57
    • Smax: 0.246
    • Smax_pos: 20
    • Smean: 0.18
    • D: 0.156
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDGEVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNEMLSENLFSAVKIYGTFKHMHVRMMPAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFTPELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVKAKIDYKDVAEEIREAE

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]