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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02958
  • pan locus tag?: SAUPAN006349000
  • symbol: SAOUHSC_02958
  • pan gene symbol?: phoB
  • synonym:
  • product: alkaline phosphatase III

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02958
  • symbol: SAOUHSC_02958
  • product: alkaline phosphatase III
  • replicon: chromosome
  • strand: +
  • coordinates: 2719684..2721108
  • length: 1425
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTTATCAGTTAAAAAAGCCACAAAATTTGCAGTTGCTAACCTTATTATCGTGTCAACG
    GTTTTTAGTTCTGTCACACCTGCAATTGCGCAATCCGATAAAAGTTCTAAAGATGAAAGT
    TTCGCGGTTGGTAATACTAAAAATCCAAAAAATGTTATCTTTTTAGTTGGCGATGGCATG
    GGTCCATCATTTAACACAGCTTATCGTTATTATAAAAATGATCCGCATGCTAAAGAAATG
    ACCCCTACTGCTTTTGATTCATATTTAAAAGGCACAAATCGTACATATTCAAATGACCCA
    AAACAGAATATTACTGATTCTGCAGCTGGCGGAACAGCCTTTAGTTCTGGTCATAAGACA
    TATAATGGTGCCATCGGTGTTGACAGTAACAAGCAGAAAGTAAAGACGGTGCTTGAACGT
    GCAAAAGAAAAAGGTAAGTCGACTGGTCTTGTTTCAACAGCTGAATTAACAGATGCAACA
    CCAGCAGCATATGCTGCACACGTGACTTCTAGAGATGATAAAAACGAAATCGCTAAACAA
    TTTTATAAAGATAAAATCAACGGAAAGCATAAAGTTGATGTTTTATTAGGCGGCGGCGCT
    AAATATTTCGGTAAAAGTAATGGCAATTTAGATAAAAAATTCAAAAAAGATGGTTACGAC
    CTTGCCACGAACAGTAAGGAGTTATCAAAATCTGATAAAGATAAAGTTCTTGGTTTGTTT
    GCAGATAAAAATATGCCACTTGCAATAGATGCTTCTAAAGACGAACCATCATTAGCTGAT
    ATGCAACAAAGTGCACTTAGTAAGCTTGAGCGCAATAAAAAAGGATTCTTTTTAATGGTC
    GAAGGTGCTTCTATAGATAAATCAGCGCATTCCAACGATATTACAGGCGTTATGTCTGAA
    ATGGAAGGTTTTGAAAAAGCATTCGATGACGCCATTCAATATGCTAAGAAACATAAAGAT
    ACACTTGTCGTTGCAACAGCAGATCACTCTACTGGTGGTCTAACAATTGGTAAAGATAAA
    GGATACGAATGGAATCCTCAACCGATTAAATCTATGAAACACTCTGGATCATATATGACA
    GAAAAAATGGCTAAAGGTGAAGACCCTGAAAAAGTTATTAATGAAGGTTATGGATTTGAA
    TTTTCACAAGATGATATGAAAAAGGTTAAAAAAGAGAATAAAAAGCTAAAAAGACTACTT
    AAAAAAGAAAAAGACGAAAAATCCGCAGAAGTAGAAACGCAAACAAAAGCCCTACAACAC
    GCTATTCAGAAGCCAGTAAATGATAAATCTTATACAGGGTGGACTTCCGATGGACATACA
    GGCGAGGATGTTAATACCTACGCTTACGGACCTAGATCAGAATCATTTAGTGGTAATATC
    GAAAATACAGAAAGCGCAAAAATAATATTTGATATATTCAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1425

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02958
  • symbol: SAOUHSC_02958
  • description: alkaline phosphatase III
  • length: 474
  • theoretical pI: 9.60182
  • theoretical MW: 51960
  • GRAVY: -0.759072

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Phosphorus MetabolismPhosphorus Metabolism - no subcategoryPhosphate metabolism Alkaline phosphatase (EC 3.1.3.1) 
  • PFAM:
    Alk_phosphatase (CL0088) Alk_phosphatase; Alkaline phosphatase (PF00245; HMM-score: 388.4)
    Metalloenzyme; Metalloenzyme superfamily (PF01676; HMM-score: 24.6)
    no clan definedPox_H7; Late protein H7 (PF04787; HMM-score: 15.9)
    DUF1889; Domain of unknown function (DUF1889) (PF08986; HMM-score: 14.7)
    TipAS; TipAS antibiotic-recognition domain (PF07739; HMM-score: 13.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: -0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: TPAIAQSD
  • SignalP: Signal peptide length 31 aa
    • Ymax: 0.832
    • Ymax_pos: 31
    • Cmax: 0.769
    • Cmax_pos: 31
    • Smax: 0.962
    • Smax_pos: 17
    • Smean: 0.871
    • D: 0.847
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLSVKKATKFAVANLIIVSTVFSSVTPAIAQSDKSSKDESFAVGNTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQNITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDATPAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDGYDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGFFLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGLTIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKENKKLKRLLKKEKDEKSAEVETQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYGPRSESFSGNIENTESAKIIFDIFK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]