From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02979
  • pan locus tag?: SAUPAN006375000
  • symbol: SAOUHSC_02979
  • pan gene symbol?: —
  • synonym:
  • product: N-acetylmuramoyl-L-alanine amidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02979
  • symbol: SAOUHSC_02979
  • product: N-acetylmuramoyl-L-alanine amidase
  • replicon: chromosome
  • strand: +
  • coordinates: 2744269..2746128
  • length: 1860
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGCCTAAAAATAAAATTTTAATTTATTTGCTATCAACTACGCTCGTATTACCTACTTTA
    GTTTCACCTACCGCTTATGCTGATACACCTCAAAAAGATACTACAGCTAAGACAACATCT
    CATGATTCAAAAAAATCTAATGACGATGAAACTTCTAAGGATACTACAAGTAAAGATATT
    GATAAAGCAGACAAAAATAATACAAGTAACCAAGACAATAACGACAAAAAATTTAAAACT
    ATAGACGACAGCACTTCAGACTCTAACAATATCATTGATTTTATTTATAAGAATTTACCA
    CAAACCAATATAAACCAATTGCTAACCAAAAATAAATACGATGATAATTACTCATTAACA
    ACTTTAATCCAAAACTTATTCAATTTAAATTCGGATATTTCTGATTACGAACAACCTCGT
    AATGGCGAAAAGTCAACAAATGATTCGAATAAAAACAGTGACAATAGCATCAAAAATGAC
    ACTGATACGCAATCATCTAAACAAGATAAAGCAGACAATCAAAAAGCACCTAAATCAAAC
    AATACAAAACCAAGTACATCTAATAAGCAACCAAATTCGCCAAAGCCAACACAACCTAAT
    CAATCAAATAGTCAACCAGCAAGTGACGATAAAGCAAATCAAAAATCTTCATCGAAAGAT
    AATCAATCAATGTCAGATTCGGCTTTAGACTCTATTTTGGATCAATACAGTGAAGATGCA
    AAGAAAACACAAAAAGATTATGCATCTCAATCTAAAAAAGACAAAAATGAAAAATCTAAT
    ACAAAGAATCCACAGTTACCAACACAAGATGAATTGAAACATAAATCTAAACCTGCTCAA
    TCATTCAATAACGATGTTAATCAAAAGGATACACGTGCAACATCATTATTCGAAACAGAT
    CCTAGTATATCTAACAATGATGATAGCGGACAATTTAACGTTGTTGACTCAAAAGATACA
    CGTCAATTTGTCAAATCAATTGCTAAAGATGCACATCGCATTGGTCAAGATAACGATATT
    TATGCGTCTGTCATGATTGCCCAAGCAATCTTAGAATCTGACTCAGGTCGTAGTGCTTTA
    GCTAAGTCACCAAACCATAATTTATTCGGTATCAAAGGTGCTTTTGAAGGGAATTCTGTT
    CCTTTTAACACATTAGAAGCTGATGGTAATCAATTGTATAGTATTAATGCTGGATTCCGA
    AAATATCCAAGCACGAAAGAATCACTAAAAGATTACTCTGACCTTATTAAAAATGGTATT
    GATGGCAATCGAACAATTTATAAACCAACATGGAAATCGGAAGCCGATTCTTATAAAGAT
    GCAACATCACACTTATCTAAAACATATGCTACAGATCCAAACTATGCTAAGAAATTAAAC
    AGTATTATTAAACACTATCAATTAACTCAGTTTGACGATGAACGCATGCCAGATTTAGAT
    AAATATGAACGTTCTATCAAGGATTATGATGATTCATCAGATGAATTCAAACCTTTCCGT
    GAGGTATCTGATAGTATGCCATATCCACATGGCCAATGTACTTGGTACGTATATAACCGT
    ATGAAACAATTTGGTACATCTATCTCAGGTGATTTAGGTGATGCACATAATTGGAATAAT
    CGAGCTCAATACCGTGATTATCAAGTAAGTCATACACCAAAACGTCATGCTGCTGTTGTA
    TTTGAGGCTGGACAATTTGGTGCAGATCAACATTACGGTCATGTAGCATTTGTTGAAAAA
    GTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATT
    TCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860

⊟Protein[edit | edit source]

Protein Data Bank: 5T1Q

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02979
  • symbol: SAOUHSC_02979
  • description: N-acetylmuramoyl-L-alanine amidase
  • length: 619
  • theoretical pI: 6.34359
  • theoretical MW: 69252.8
  • GRAVY: -1.13279

⊟Function[edit | edit source]

  • TIGRFAM:
    flagellar rod assembly protein/muramidase FlgJ (TIGR02541; HMM-score: 81.9)
    and 1 more
    pentapeptide MXKDX repeat protein (TIGR02953; HMM-score: 15.7)
  • TheSEED  :
    • N-acetylmuramoyl-L-alanine amidase, family 4
  • PFAM:
    Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 75.2)
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 66.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helix: 1
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: PTAYADTP
  • SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.99354
    • TAT(Tat/SPI): 0.004521
    • LIPO(Sec/SPII): 0.001352
    • Cleavage Site: CS pos: 27-28. AYA-DT. Pr: 0.9885
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSKKSNDDETSKDTTSKDIDKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQTNINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKNDTDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSSKDNQSMSDSALDSILDQYSEDAKKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKSKPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRIGQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQLYSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYATDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPYPHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQFGADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Ian R Siboo, Donald O Chaffin, Craig E Rubens, Paul M Sullam
Characterization of the accessory Sec system of Staphylococcus aureus.
J Bacteriol: 2008, 190(18);6188-96
[PubMed:18621893] [WorldCat.org] [DOI] (I p)Mark J J B Sibbald, Theresa Winter, Magdalena M van der Kooi-Pol, G Buist, E Tsompanidou, Tjibbe Bosma, Tina Schäfer, Knut Ohlsen, Michael Hecker, Haike Antelmann, Susanne Engelmann, Jan Maarten van Dijl
Synthetic effects of secG and secY2 mutations on exoproteome biogenesis in Staphylococcus aureus.
J Bacteriol: 2010, 192(14);3788-800
[PubMed:20472795] [WorldCat.org] [DOI] (I p)

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAOUHSC_02979&oldid=101420"