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04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40

NCBI: 27-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SAP017 [new locus tag: SA_RS00075 ]
  • pan locus tag?:
  • symbol: arsB
  • pan gene symbol?:
  • synonym:
  • product: arsenic efflux pump protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAP017 [new locus tag: SA_RS00075 ]
  • symbol: arsB
  • product: arsenic efflux pump protein
  • replicon: pN315
  • strand: +
  • coordinates: 14421..15710
  • length: 1290
  • essential: unknown

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACTATTTTAGCAATTGTAATTTTTCTTTTAACTTTAACCTTTGTGATTTGGCAACCA
    AAAGGTTTAGATATTGGTATTACAGCTTTAATTGGAGCGGTTGTTGCTATCATTACAGGA
    GTCGTAAGTCTTTCTGATGTATTAGAAGTAACAGGTATTGTTTGGAATGCAACTTTAACT
    TTTGTGGCTGTTATTCTTATTTCATTAATATTAGATGAAATTGGTTTTTTTGAATGGTCT
    GCGATACATATGGTCAAGGCTTCAAACGGTAATGGCTTAAAAATGTTTGTTTTTATTATG
    TTACTTGGGGCAATTGTAGCAGCATTTTTCGCAAATGATGGTGCAGCTTTAATCTTAACG
    CCTATTGTATTAGCGATGGTAAGGAATCTAGGATTTAATCAAAAAGTGATTTTCCCCTTT
    ATTATTGCCAGTGGTTTTATTGCTGATACTACATCACTTCCCTTAATTGTAAGTAACTTA
    GTTAATATCGTTTCTGCAGATTACTTCGATATTGGATTTATTGAATATTTTAGCCGAATG
    ATCATTCCTAATATATTCTCTCTGATTGCTAGTATTCTCGTTTTATGGTTATATTTCAGA
    AAATCCATACCTAAAACGTTCGATACAGAAAATCTATCAGATCCTAAAAGTGCAATTAAA
    GATTCTAAGTTATTTAAGCTTTCATGGATAGTATTAGCAGTACTACTCGTTGGATATCTT
    GTTAGCGAGTTTATCCAAATTCCTGTATCAATTATTGCTGGTATTATTGCTTTTATCTTT
    GTAATATTAGCTCGTAAATCTAAAGCAGTTCATACAAAACAAGTGATTAAAGGTGCACCA
    TGGAATATCGTTGTATTCTCTATTGGTATGTATCTTGTTGTGTTTGGACTAAAAAATGTA
    GGTATTACAACGGTCCTTGGGGATGTTTTAACAAACATTTCAAATTATGGGTTATTTAGC
    AGCATCATGGGTATGGGCTTTATAGCTGCTTTCTTATCTTCGATTATGAACAACATGCCA
    ACTGTTTTAATAGATGCAATAGCGATTGGCCAATCCAGTGCTACAGGAATATTAAAAGAA
    GGCATGGTTTATGCGAATGTCATAGGTTCTGATTTAGGACCTAAAATTACGCCAATTGGT
    TCTTTAGCAACATTATTGTGGCTACATGTCTTAACACAAAAAGGTGTGAAGATTTCGTGG
    GGAACATACTTTAAAACTGGAATTATCATCACTATTCCAGTCCTATTTGTAACACTCTTA
    GGTTTATACCTTACACTAATCATATTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1290

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAP017 [new locus tag: SA_RS00075 ]
  • symbol: ArsB
  • description: arsenic efflux pump protein
  • length: 429
  • theoretical pI: 9.55902
  • theoretical MW: 46469.7
  • GRAVY: 1.15291

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesDetoxificationarsenite/antimonite efflux pump membrane protein (TIGR00935; HMM-score: 754.7)
    MetabolismTransport and binding proteinsOtherarsenite/antimonite efflux pump membrane protein (TIGR00935; HMM-score: 754.7)
    MetabolismTransport and binding proteinsCations and iron carrying compoundstransporter, divalent anion:Na+ symporter (DASS) family (TIGR00785; HMM-score: 52.7)
    MetabolismTransport and binding proteinsAnionstransporter, divalent anion:Na+ symporter (DASS) family (TIGR00785; HMM-score: 52.7)
  • TheSEED:  
    Virulence, Disease and DefenseResistance to antibiotics and toxic compoundsArsenic resistance Arsenic efflux pump protein 
  • PFAM:
    IT (CL0182) ArsB; Arsenical pump membrane protein (PF02040; HMM-score: 651.9)
    CitMHS; Citrate transporter (PF03600; HMM-score: 96)
    Na_sulph_symp; Sodium:sulfate symporter transmembrane region (PF00939; HMM-score: 40.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.155
    • Ymax_pos: 11
    • Cmax: 0.146
    • Cmax_pos: 23
    • Smax: 0.34
    • Smax_pos: 2
    • Smean: 0.223
    • D: 0.181
  • predicted transmembrane helices (TMHMM): 11

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTILAIVIFLLTLTFVIWQPKGLDIGITALIGAVVAIITGVVSLSDVLEVTGIVWNATLTFVAVILISLILDEIGFFEWSAIHMVKASNGNGLKMFVFIMLLGAIVAAFFANDGAALILTPIVLAMVRNLGFNQKVIFPFIIASGFIADTTSLPLIVSNLVNIVSADYFDIGFIEYFSRMIIPNIFSLIASILVLWLYFRKSIPKTFDTENLSDPKSAIKDSKLFKLSWIVLAVLLVGYLVSEFIQIPVSIIAGIIAFIFVILARKSKAVHTKQVIKGAPWNIVVFSIGMYLVVFGLKNVGITTVLGDVLTNISNYGLFSSIMGMGFIAAFLSSIMNNMPTVLIDAIAIGQSSATGILKEGMVYANVIGSDLGPKITPIGSLATLLWLHVLTQKGVKISWGTYFKTGIIITIPVLFVTLLGLYLTLIIF

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: ArsR
    ArsR (TF) important in Arsenic resistance  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]