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COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 27-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SAP017 [new locus tag: SA_RS00075 ]
  • pan locus tag?:
  • symbol: arsB
  • pan gene symbol?:
  • synonym:
  • product: arsenic efflux pump protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAP017 [new locus tag: SA_RS00075 ]
  • symbol: arsB
  • product: arsenic efflux pump protein
  • replicon: pN315
  • strand: +
  • coordinates: 14421..15710
  • length: 1290
  • essential: unknown

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACTATTTTAGCAATTGTAATTTTTCTTTTAACTTTAACCTTTGTGATTTGGCAACCA
    AAAGGTTTAGATATTGGTATTACAGCTTTAATTGGAGCGGTTGTTGCTATCATTACAGGA
    GTCGTAAGTCTTTCTGATGTATTAGAAGTAACAGGTATTGTTTGGAATGCAACTTTAACT
    TTTGTGGCTGTTATTCTTATTTCATTAATATTAGATGAAATTGGTTTTTTTGAATGGTCT
    GCGATACATATGGTCAAGGCTTCAAACGGTAATGGCTTAAAAATGTTTGTTTTTATTATG
    TTACTTGGGGCAATTGTAGCAGCATTTTTCGCAAATGATGGTGCAGCTTTAATCTTAACG
    CCTATTGTATTAGCGATGGTAAGGAATCTAGGATTTAATCAAAAAGTGATTTTCCCCTTT
    ATTATTGCCAGTGGTTTTATTGCTGATACTACATCACTTCCCTTAATTGTAAGTAACTTA
    GTTAATATCGTTTCTGCAGATTACTTCGATATTGGATTTATTGAATATTTTAGCCGAATG
    ATCATTCCTAATATATTCTCTCTGATTGCTAGTATTCTCGTTTTATGGTTATATTTCAGA
    AAATCCATACCTAAAACGTTCGATACAGAAAATCTATCAGATCCTAAAAGTGCAATTAAA
    GATTCTAAGTTATTTAAGCTTTCATGGATAGTATTAGCAGTACTACTCGTTGGATATCTT
    GTTAGCGAGTTTATCCAAATTCCTGTATCAATTATTGCTGGTATTATTGCTTTTATCTTT
    GTAATATTAGCTCGTAAATCTAAAGCAGTTCATACAAAACAAGTGATTAAAGGTGCACCA
    TGGAATATCGTTGTATTCTCTATTGGTATGTATCTTGTTGTGTTTGGACTAAAAAATGTA
    GGTATTACAACGGTCCTTGGGGATGTTTTAACAAACATTTCAAATTATGGGTTATTTAGC
    AGCATCATGGGTATGGGCTTTATAGCTGCTTTCTTATCTTCGATTATGAACAACATGCCA
    ACTGTTTTAATAGATGCAATAGCGATTGGCCAATCCAGTGCTACAGGAATATTAAAAGAA
    GGCATGGTTTATGCGAATGTCATAGGTTCTGATTTAGGACCTAAAATTACGCCAATTGGT
    TCTTTAGCAACATTATTGTGGCTACATGTCTTAACACAAAAAGGTGTGAAGATTTCGTGG
    GGAACATACTTTAAAACTGGAATTATCATCACTATTCCAGTCCTATTTGTAACACTCTTA
    GGTTTATACCTTACACTAATCATATTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1290

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAP017 [new locus tag: SA_RS00075 ]
  • symbol: ArsB
  • description: arsenic efflux pump protein
  • length: 429
  • theoretical pI: 9.55902
  • theoretical MW: 46469.7
  • GRAVY: 1.15291

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Detoxification arsenite/antimonite efflux pump membrane protein (TIGR00935; HMM-score: 754.7)
    Metabolism Transport and binding proteins Other arsenite/antimonite efflux pump membrane protein (TIGR00935; HMM-score: 754.7)
    and 2 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds transporter, divalent anion:Na+ symporter (DASS) family (TIGR00785; HMM-score: 52.7)
    Metabolism Transport and binding proteins Anions transporter, divalent anion:Na+ symporter (DASS) family (TIGR00785; HMM-score: 52.7)
  • TheSEED  :
    • Arsenite/antimonite:H+ antiporter ArsB (EC 7.3.2.7)
    Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Arsenic resistance  Arsenic efflux pump protein
  • PFAM:
    IT (CL0182) ArsB; Arsenical pump membrane protein (PF02040; HMM-score: 651.9)
    and 2 more
    CitMHS; Citrate transporter (PF03600; HMM-score: 96)
    Na_sulph_symp; Sodium:sulfate symporter transmembrane region (PF00939; HMM-score: 40.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016996
    • TAT(Tat/SPI): 0.00013
    • LIPO(Sec/SPII): 0.011195
  • predicted transmembrane helices (TMHMM): 11

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTILAIVIFLLTLTFVIWQPKGLDIGITALIGAVVAIITGVVSLSDVLEVTGIVWNATLTFVAVILISLILDEIGFFEWSAIHMVKASNGNGLKMFVFIMLLGAIVAAFFANDGAALILTPIVLAMVRNLGFNQKVIFPFIIASGFIADTTSLPLIVSNLVNIVSADYFDIGFIEYFSRMIIPNIFSLIASILVLWLYFRKSIPKTFDTENLSDPKSAIKDSKLFKLSWIVLAVLLVGYLVSEFIQIPVSIIAGIIAFIFVILARKSKAVHTKQVIKGAPWNIVVFSIGMYLVVFGLKNVGITTVLGDVLTNISNYGLFSSIMGMGFIAAFLSSIMNNMPTVLIDAIAIGQSSATGILKEGMVYANVIGSDLGPKITPIGSLATLLWLHVLTQKGVKISWGTYFKTGIIITIPVLFVTLLGLYLTLIIF

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: ArsR (repression) regulon
    ArsR(TF)important in Arsenic resistance; RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]