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04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40

NCBI: 27-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SAP028 [new locus tag: SA_RS00130 ]
  • pan locus tag?:
  • symbol: SAP028
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAP028 [new locus tag: SA_RS00130 ]
  • symbol: SAP028
  • product: hypothetical protein
  • replicon: pN315
  • strand: +
  • coordinates: 22517..22756
  • length: 240
  • essential: unknown

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    ATGAAATTAGCTGAAGCTATAAAAGAACAGAGAGAATTAAAAAGATGGTCTCAAGAGGAA
    TTAGCCAATATATTAAAGGTTTCCAGACAAAGTGTTTCTAAATGGGAAAGTGCCAAAAAC
    TATCCTTCCTTAGATATTTTGATTGCTATGAGCGATTTATTTGGAATCTCTTTAGAGCAT
    TTAATTAAAGGAGATGCACGTTTTAAAAAAGTTATTTTAGAGGGAAATTATAGAGAGTAA
    60
    120
    180
    240

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAP028 [new locus tag: SA_RS00130 ]
  • symbol: SAP028
  • description: hypothetical protein
  • length: 79
  • theoretical pI: 9.63142
  • theoretical MW: 9191.6
  • GRAVY: -0.444304

Function[edit source | edit]

  • TIGRFAM:
    Signal transductionRegulatory functionsDNA interactionstranscriptional regulator, y4mF family (TIGR03070; HMM-score: 37)
    and 4 more
    putative zinc finger/helix-turn-helix protein, YgiT family (TIGR03830; HMM-score: 27.4)
    Hypothetical proteinsConservedTIGR00270 family protein (TIGR00270; HMM-score: 24.6)
    RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 16.6)
    Signal transductionRegulatory functionsDNA interactionsDNA-binding protein, Tfx family (TIGR00721; HMM-score: 15.1)
  • TheSEED:  
    Transcriptional regulator, XRE family 
  • PFAM:
    HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 57.8)
    HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 46.6)
    and 16 more
    HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 34.8)
    Phage_CI_repr; Bacteriophage CI repressor helix-turn-helix domain (PF07022; HMM-score: 28)
    HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 22.7)
    HTH_37; Helix-turn-helix domain (PF13744; HMM-score: 20.9)
    HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 18.8)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 18.1)
    P22_Cro; DNA-binding transcriptional regulator Cro (PF14549; HMM-score: 17.4)
    YdaS_antitoxin; Putative antitoxin of bacterial toxin-antitoxin system, YdaS/YdaT (PF15943; HMM-score: 17.1)
    Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 16.9)
    HTH_25; Helix-turn-helix domain (PF13413; HMM-score: 16.4)
    HTH_17; Helix-turn-helix domain (PF12728; HMM-score: 15.5)
    HTH_11; HTH domain (PF08279; HMM-score: 14.5)
    HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 14.1)
    HTH_Tnp_1_2; Helix-turn-helix of insertion element transposase (PF13022; HMM-score: 13.8)
    Phage_terminase; Phage terminase small subunit (PF10668; HMM-score: 13.6)
    DUF1323; Putative transcription regulator (DUF1323) (PF07037; HMM-score: 13)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.11
    • Ymax_pos: 39
    • Cmax: 0.14
    • Cmax_pos: 39
    • Smax: 0.126
    • Smax_pos: 2
    • Smean: 0.085
    • D: 0.1
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKLAEAIKEQRELKRWSQEELANILKVSRQSVSKWESAKNYPSLDILIAMSDLFGISLEHLIKGDARFKKVILEGNYRE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]