NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0033 [new locus tag: SAUSA300_RS00170 ]
• pan locus tag^?: SAUPAN000102000
• symbol: SAUSA300_0033
• pan gene symbol^?: mecR1
• synonym:
• product: methicillin-resistance MecR1 regulatory protein

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0033 [new locus tag: SAUSA300_RS00170 ]
• symbol: SAUSA300_0033
• product: methicillin-resistance MecR1 regulatory protein
• replicon: chromosome
• strand: +
• coordinates: 41233..42219
• length: 987
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913613 NCBI
• RefSeq: YP_492753 NCBI
• MicrobesOnline: 1291548 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  
  GTGTTATCATCTTTTTTAATGTTAAGTATAATCAGTTCATTGCTCACGATATGTGTAATT
  TTTTTAGTGAGAATGCTCTATATAAAATATACTCAAAATATTATGTCACATAAGATTTGG
  TTATTAGTGCTCGTCTCCACGTTAATTCCATTAATACCATTTTACAAAATATCGAATTTT
  ACATTTTCAAAAGATATGATGAATCGAAATGTATCTGACACGACTTCTTCGGTTAGTCAT
  ATGTTAGATGGTCAACAATCATCTGTTACGAAAGACTTAGCAATTAATGTTAATCAGTTT
  GAGACCTCAAATATAACGTATATGATTCTTTTGATATGGGTATTTGGTAGTTTGTTGTGC
  TTATTTTATATGATTAAGGCATTCCGACAAATTGATGTTATTAAAAGTTCGTCATTGGAA
  TCGTCATATCTTAATGAACGACTTAAAGTATGTCAAAGTAAGATGCAGTTCTACAAAAAG
  CATATAACAATTAGTTATAGTTCAAACATTGATAATCCGATGGTATTTGGTTTAGTGAAA
  TCCCAAATTGTACTACCAACTGTCGTAGTCGAAACCATGAATGACAAAGAAATTGAATAT
  ATTATTCTACATGAACTATCACATGTGAAAAGTCATGACTTAATATTCAACCAGCTTTAT
  GTTGTTTTTAAAATGATATTCTGGTTTAATCCTGCACTATATATAAGTAAAACAATGATG
  GACAATGACTGTGAAAAAGTATGTGATAGAAACGTTTTAAAAATTTTGAATCGCCATGAA
  CATATACGTTATGGTGAATCGATATTAAAATGCTCTATTTTAAAATCTCAGCACATAAAT
  AATGTGGCAGCACAATATTTACTAGGTTTTAATTCAAATATTAAAGAACGTGTTAAGTAT
  ATTGCACTTTATGATTCAATGCCTAAACCTAATCGAAACAAGCGTATTGTTGCGTATATT
  GTATGTAGTATATCGAGCTTCACATGA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  987
  

⊟Protein

⊟General

• locus tag: SAUSA300_0033 [new locus tag: SAUSA300_RS00170 ]
• symbol: SAUSA300_0033
• description: methicillin-resistance MecR1 regulatory protein
• length: 328
• theoretical pI: 9.43713
• theoretical MW: 38128.9
• GRAVY: 0.244817

⊟Function

• reaction:
• TIGRFAM:
• TheSEED:  
  Methicillin resistance regulatory sensor-transducer MecR1, truncated 
• PFAM:
  Peptidase_MA (CL0126) Peptidase_M56; BlaR1 peptidase M56 (PF05569; HMM-score: 338.5)
  Peptidase_M48; Peptidase family M48 (PF01435; HMM-score: 27.6)
  no clan definedCaiF_GrlA; CaiF/GrlA transcriptional regulator (PF07180; HMM-score: 13.9)
  Peptidase_MA (CL0126) DUF45; Protein of unknown function DUF45 (PF01863; HMM-score: 11.6)
  no clan definedDUF1516; Protein of unknown function (DUF1516) (PF07457; HMM-score: 10.3)
  O-anti_assembly (CL0499) Wzy_C; O-Antigen ligase (PF04932; HMM-score: 10.2)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 3
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular Possibility: 0.17
  • Signal Peptide Possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.223
  • Ymax_pos: 11
  • Cmax: 0.118
  • Cmax_pos: 37
  • Smax: 0.623
  • Smax_pos: 3
  • Smean: 0.494
  • D: 0.329
• predicted transmembrane helices (TMHMM): 3

⊟Accession numbers

• GI: 87162304 NCBI
• RefSeq: YP_492753 NCBI
• UniProt: A0A0H2XIW9 UniProt

⊟Protein sequence

• MLSSFLMLSIISSLLTICVIFLVRMLYIKYTQNIMSHKIWLLVLVSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFETSNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKKHITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQLYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKSQHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0033 > SAUSA300_0035

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

You are kindly invited to share additional interesting facts.