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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0264 [new locus tag: SAUSA300_RS01415 ]
  • pan locus tag?: SAUPAN001156000
  • symbol: SAUSA300_0264
  • pan gene symbol?: rbsU
  • synonym:
  • product: ribose transporter RbsU

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0264 [new locus tag: SAUSA300_RS01415 ]
  • symbol: SAUSA300_0264
  • product: ribose transporter RbsU
  • replicon: chromosome
  • strand: -
  • coordinates: 315859..316740
  • length: 882
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAGCATTGTTGCATTACTTATCGGGTTAGGCCCCTTAATTGGCTGGGGCTTCTTCCCA
    ACAGTCGCTTCAAAGTTTGGTGGTAAACCTGTACATCAAATTATCGGTGCTACTGTAGGT
    ACGTTAATCTTCGCTATTATATTAGCCGTAGTCACATCAAGTGGCTTACCTACTGGAACC
    AATTTGCTATTCGCCTTATTATCAGGTGCAGGATGGGGATTCGGACAAATCATTACATTT
    AAAGCGTTCGAATTAGTCGGCTCATCTCGTGCCATGCCAGTCACAACAGCATTCCAATTA
    TTAGGCGCATCTTTATGGGGTGTCTTTGCATTAGGAAATTGGCCAGGCATTGGTCATAAA
    ATCATTGGATTTACAGCTTTAGTCGTTATTCTAATTGGAGCGCGTATGACAGTTTGGAGT
    GAACGCAAAGAAGCAAGTAACGCCAAAAATTTACGTCGTGCAGTGGTACTTCTGTTAATT
    GGTGAATTTGGATACTGGTTATATTCAGCTGCACCGCAAGCAACTTCTATTGATGGCCTA
    ACTGCCTTTTTACCTCAAGCAATGGGTATGGTAATTGTTGCAGTCATTTATGGCTTTATG
    AATATGAAAGCAGAGAATCCATTCCGTAATAAAATTACGTGGTTACAAATTATTTCAGGT
    TTCTTCTTTGCATTTGGTGCTTTAACATATCTTATTTCAGCACAACCTAATATGAATGGT
    TTAGCAACTGGATTTATTCTTTCTCAAACATCCGTTGTGCTTGCTACATTAACTGGTATT
    TATTTCTTAAAACAACATAAAACGTCAAAAGAAATGGTTATTACAATCATCGGCTTAGTA
    CTCATTTTAGTAGCCGCTTCTGTTACAGTATTTATAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    882

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0264 [new locus tag: SAUSA300_RS01415 ]
  • symbol: SAUSA300_0264
  • description: ribose transporter RbsU
  • length: 293
  • theoretical pI: 10.7683
  • theoretical MW: 31209
  • GRAVY: 0.902389

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids RhaT L-rhamnose-proton symporter family protein (TIGR00776; HMM-score: 256.8)
  • TheSEED  :
    • Putative ribose uptake protein RbsU, GRP transporter family (TC 2.A.7.5)
    Carbohydrates Monosaccharides D-ribose utilization  Putative ribose uptake protein RbsU, GRP transporter family (TC 2.A.7.5)
  • PFAM:
    DMT (CL0184) Sugar_transport; Sugar transport protein (PF06800; HMM-score: 364.1)
    and 2 more
    TMEM144; Transmembrane family, TMEM144 of transporters (PF07857; HMM-score: 15.8)
    no clan defined HIG_1_N; Hypoxia induced protein conserved region (PF04588; HMM-score: 9.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.048536
    • TAT(Tat/SPI): 0.003463
    • LIPO(Sec/SPII): 0.032429
  • predicted transmembrane helices (TMHMM): 10

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIFAIILAVVTSSGLPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLGASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLIGEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQIISGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITIIGLVLILVAASVTVFIK

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]