NCBI: 10-JUN-2013
Contents
⊟Summary[edit source | edit]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_0777 [new locus tag: SAUSA300_RS04190 ]
- pan locus tag?: SAUPAN002802000
- symbol: SAUSA300_0777
- pan gene symbol?: cspC
- synonym:
- product: cold shock protein
⊟Genome View[edit source | edit]
⊟Gene[edit source | edit]
⊟General[edit source | edit]
- type: CDS
- locus tag: SAUSA300_0777 [new locus tag: SAUSA300_RS04190 ]
- symbol: SAUSA300_0777
- product: cold shock protein
- replicon: chromosome
- strand: +
- coordinates: 867258..867458
- length: 201
- essential: unknown other strains
⊟Accession numbers[edit source | edit]
- Gene ID: 3914809 NCBI
- RefSeq: YP_493477 NCBI
- MicrobesOnline: 1292292 MicrobesOnline
⊟Phenotype[edit source | edit]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit source | edit]
- 1
61
121
181ATGAATAACGGTACAGTTAAATGGTTTAATGCAGAAAAAGGTTTTGGTTTCATCGAAAGA
GAAGATGGTAGCGACGTATTCGTACACTTCTCAGCAATCGCTGAAGATGGATACAAATCA
TTAGAAGAAGGCCAAAAAGTTGAATTCGACATCGTTGAAGGCGACCGTGGCGAGCAAGCT
GCAAACGTAGTTAAAATGTAA60
120
180
201
⊟Protein[edit source | edit]
⊟General[edit source | edit]
- locus tag: SAUSA300_0777 [new locus tag: SAUSA300_RS04190 ]
- symbol: SAUSA300_0777
- description: cold shock protein
- length: 66
- theoretical pI: 4.18132
- theoretical MW: 7374.06
- GRAVY: -0.513636
⊟Function[edit source | edit]
- reaction:
- TIGRFAM: Cellular processesAdaptations to atypical conditionscold shock domain protein CspD (TIGR02381; HMM-score: 91.9)DNA metabolismDNA replication, recombination, and repaircold shock domain protein CspD (TIGR02381; HMM-score: 91.9)TranscriptionDegradation of RNAVacB and RNase II family 3'-5' exoribonucleases (TIGR00358; EC 3.1.13.1; HMM-score: 12.1)
- TheSEED:
- PFAM: OB (CL0021) CSD; 'Cold-shock' DNA-binding domain (PF00313; HMM-score: 108.8)S1; S1 RNA binding domain (PF00575; HMM-score: 18)OB_RNB; Ribonuclease B OB domain (PF08206; HMM-score: 15.8)
⊟Structure, modifications & interactions[edit source | edit]
- domains:
- modifications:
- cofactors:
- effectors:
- protein partners:
⊟Localization[edit source | edit]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular Possibility: 1
- Signal Peptide Possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- Ymax: 0.099
- Ymax_pos: 61
- Cmax: 0.116
- Cmax_pos: 35
- Smax: 0.127
- Smax_pos: 2
- Smean: 0.073
- D: 0.089
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit source | edit]
⊟Protein sequence[edit source | edit]
- MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEGQKVEFDIVEGDRGEQAANVVKM
⊟Experimental data[edit source | edit]
- experimentally validated: no data available
⊟Expression & Regulation[edit source | edit]
⊟Operon[edit source | edit]
- MicrobesOnline: SAUSA300_0777 no polycistronic organisation predicted
⊟Regulation[edit source | edit]
- sigma factor:
- regulator:
⊟Transcription pattern[edit source | edit]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit source | edit]
- Aureolib: no data available
⊟Protein stability[edit source | edit]
- half-life: no data available
⊟Biological Material[edit source | edit]
⊟Mutants[edit source | edit]
⊟Expression vector[edit source | edit]
⊟lacZ fusion[edit source | edit]
⊟GFP fusion[edit source | edit]
⊟two-hybrid system[edit source | edit]
⊟FLAG-tag construct[edit source | edit]
⊟Antibody[edit source | edit]
⊟Other Information[edit source | edit]
You are kindly invited to share additional interesting facts.
⊟Literature[edit source | edit]
⊟References[edit source | edit]
⊟Relevant publications[edit source | edit]