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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0834 [new locus tag: SAUSA300_RS04500 ]
  • pan locus tag?: SAUPAN003028000
  • symbol: SAUSA300_0834
  • pan gene symbol?:
  • synonym:
  • product: D-isomer specific 2-hydroxyacid dehydrogenase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0834 [new locus tag: SAUSA300_RS04500 ]
  • symbol: SAUSA300_0834
  • product: D-isomer specific 2-hydroxyacid dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 912873..913832
  • length: 960
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    TTGGTAAAAATAGTTGTTTCGAGGAAAATTCCAGATAAATTTTATCAACAATTAAGTAAA
    CTTGGTGACGTTGTTATGTGGCAAAAATCATTAGTGCCTATGCCTAAAGATCAATTTGTG
    ACAGCACTTCGTGACGCAGATGCTTGTTTTATTACATTAAGTGAACAGATCGATGCAGAA
    ATTTTAGCGCAATCACCAAATTTAAAAGTAATTGCGAATATGGCTGTAGGATATGACAAC
    ATCGATGTTGAAAGTGCAACAGCGAATAACGTGGTTGTCACGAATACACCAAATGTACTT
    ACTGAAACAACTGCAGAATTAGGATTTACATTAATGCTTGCTATAGCACGCCGTATTGTA
    GAAGCTGAAAAATATGTAGAAGCAGATGCATGGCAAAGCTGGGGTCCTTATTTATTGTCA
    GGTAAAGATGTCTTCAATTCAACTATTGGAATATATGGTATGGGAGATATTGGTAAAGCT
    TTTGCAAGAAGGTTGCAAGGGTTTAATACTAATATTCTTTATCATAATCGATCAAGACAT
    AAAGATGCAGAGGCGGACTTTAATGCAACATATGTTTCTTTTGAAACGTTGTTAGCAGAA
    AGTGATTTTATCATCTGTACAGCGCCACTTACAAAAGAAACACATCATAAATTTAATGCT
    GAAGCATTTGAACAAATGAAAAATGATGCAATTTTTATTAATATCGGTAGAGGACAAATT
    GTAGATGAAACAGCATTAATCGATGCACTAGACAATAAAGAAATTTTAGCATGTGGTTTA
    GATGTATTAGCAAATGAACCGATTGATCATACACATCCATTAATGGGACGTGATAATGTT
    CTGATTACACCACACATTGGTAGCGCATCAGTAACAACACGGGACAATATGATTCAATTA
    TGTATTAATAATATAGAAGCGGTTATGACAAATCAGGTACCACATACTCCAGTAAATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0834 [new locus tag: SAUSA300_RS04500 ]
  • symbol: SAUSA300_0834
  • description: D-isomer specific 2-hydroxyacid dehydrogenase
  • length: 319
  • theoretical pI: 4.74512
  • theoretical MW: 35357.1
  • GRAVY: -0.0435737

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Amino acid biosynthesis Serine family phosphoglycerate dehydrogenase (TIGR01327; EC 1.1.1.95; HMM-score: 213.3)
    and 2 more
    Metabolism Energy metabolism Amino acids and amines adenosylhomocysteinase (TIGR00936; EC 3.3.1.1; HMM-score: 21.5)
    Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 17.5)
  • TheSEED  :
    • Glyoxylate reductase (EC 1.1.1.26)
    • Glyoxylate reductase (NADP+) (EC 1.1.1.79)
    • Hydroxypyruvate reductase (EC 1.1.1.81)
    Carbohydrates Central carbohydrate metabolism Glycolate, glyoxylate interconversions  Glyoxylate reductase (EC 1.1.1.26)
    and 3 more
    Carbohydrates Central carbohydrate metabolism Glycolate, glyoxylate interconversions  Glyoxylate reductase (EC 1.1.1.79)
    Carbohydrates Central carbohydrate metabolism Glycolate, glyoxylate interconversions  Hydroxypyruvate reductase (EC 1.1.1.81)
    Carbohydrates Organic acids Glycerate metabolism  Hydroxypyruvate reductase (EC 1.1.1.81)
  • PFAM:
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 208.8)
    and 5 more
    Form_Glyc_dh (CL0325) 2-Hacid_dh; D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain (PF00389; HMM-score: 105.6)
    NADP_Rossmann (CL0063) NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 27.2)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 24)
    AdoHcyase_NAD; S-adenosyl-L-homocysteine hydrolase, NAD binding domain (PF00670; HMM-score: 22.2)
    IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 14.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002252
    • TAT(Tat/SPI): 0.000129
    • LIPO(Sec/SPII): 0.000229
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFVTALRDADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTETTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKAFARRLQGFNTNILYHNRSRHKDAEADFNATYVSFETLLAESDFIICTAPLTKETHHKFNAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMGRDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]