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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0887 [new locus tag: SAUSA300_RS04785 ]
  • pan locus tag?: SAUPAN003133000
  • symbol: oppB
  • pan gene symbol?: opp3B
  • synonym:
  • product: oligopeptide ABC transporter permease

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0887 [new locus tag: SAUSA300_RS04785 ]
  • symbol: oppB
  • product: oligopeptide ABC transporter permease
  • replicon: chromosome
  • strand: +
  • coordinates: 974149..975075
  • length: 927
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGGGAAATATATTTTCAAACGATTTATTTATATGCTTATTTCTTTATTTATTATTATT
    ACAATTACATTTTTCTTAATGAAATTAATGCCAGGTTCGCCATTTAACGATGCTAAATTA
    AATGCTGAACAAAAAGAAATTTTAAATGAAAAATATGGATTAAATGATCCTGTAGCTACG
    CAGTATTTACATTATTTAAAAAATGTTGTTACAGGCGATTTTGGTAATTCATTCCAGTAT
    CATAATCAACCTGTGTGGGATTTGATTAAACCGAGACTACTACCTTCTTTTGAAATGGGT
    CTTACAGCAATGTTCATCGGTGTGATACTGGGACTTATTTTAGGTGTTGCAGCAGCTACT
    AAACAAAATTCTTGGGTTGACTATACAACTACAGTTATTTCAGTTATTGCAGTATCTGTA
    CCATCTTTTGTACTTGCTGTACTTTTACAATATGTATTTGCAGTTAAATTAAGATGGTTC
    CCAGTAGCTGGATGGGAAGGTTTTTCGACCGCGGTATTACCGTCACTTGCATTATCTGCA
    GCTGTTTTAGCAACTGTCGCCAGATACATAAGAGCAGAGATGATAGAGGTATTAAGTTCA
    GACTATATTTTATTAGCGAGAGCTAAAGGTAATTCGACAATGCGTGTACTTTTTGGACAT
    GCACTTAGAAATGCTTTAATTCCAATTATTACAATTATCGTTCCCATGTTAGCAAGTATT
    TTAACAGGCACTTTAACAATTGAAAATATTTTTGGAGTTCCTGGATTAGGGGATCAATTC
    GTACGTTCAATTACAACAAATGATTTCTCAGTAATCATGGCAATCACACTATTATTTAGC
    ACACTGTTTATCGTTTCTATTTTTATTGTAGATATTTTGTACGGTGTGATAGATCCACGA
    ATTCGTGTTCAAGGAGGTAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    927

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0887 [new locus tag: SAUSA300_RS04785 ]
  • symbol: OppB
  • description: oligopeptide ABC transporter permease
  • length: 308
  • theoretical pI: 9.822
  • theoretical MW: 34142.4
  • GRAVY: 0.719156

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 228.4)
    and 2 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikC (TIGR02790; EC 3.6.3.24; HMM-score: 19.6)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 15.3)
  • TheSEED  :
    • Oligopeptide ABC transporter (EC 7.4.2.6), permease protein OppB
    Membrane Transport ABC transporters ABC transporter oligopeptide (TC 3.A.1.5.1)  Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1)
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 126.6)
    and 2 more
    no clan defined NPCC; Nuclear pore complex component (PF08058; HMM-score: 11.3)
    Renin_r; Renin receptor-like protein (PF07850; HMM-score: 9)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.294557
    • TAT(Tat/SPI): 0.003898
    • LIPO(Sec/SPII): 0.007787
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQKEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLTAMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWFPVAGWEGFSTAVLPSLALSAAVLATVARYIRAEMIEVLSSDYILLARAKGNSTMRVLFGHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAITLLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]

Aurelia Hiron, Elise Borezée-Durant, Jean-Christophe Piard, Vincent Juillard
Only one of four oligopeptide transport systems mediates nitrogen nutrition in Staphylococcus aureus.
J Bacteriol: 2007, 189(14);5119-29
[PubMed:17496096] [WorldCat.org] [DOI] (P p)