NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0930 [new locus tag: SAUSA300_RS04995 ]
• pan locus tag^?: SAUPAN003213000
• symbol: SAUSA300_0930
• pan gene symbol^?: lplA1
• synonym:
• product: lipoate-protein ligase A family protein

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0930 [new locus tag: SAUSA300_RS04995 ]
• symbol: SAUSA300_0930
• product: lipoate-protein ligase A family protein
• replicon: chromosome
• strand: -
• coordinates: 1020145..1021131
• length: 987
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915077 NCBI
• RefSeq: YP_493628 NCBI
• MicrobesOnline: 1292445 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  901
  961
  
  ATGAAATTCATTAGTAATAATAATATTACAGATCCAACTTTAAACTTAGCAATGGAAGAA
  TATGTTTTAAAAAATTTACCAGCAGAAGAAAGTTACTTTTTATTTTACATAAATAGACCA
  TCTATCATTGTTGGAAAGAATCAAAATACGATAGAGGAAGTAAATCAGACATATATCGAT
  GCTCACAACATCGATGTAGTAAGAAGAATTTCTGGTGGTGGCGCTGTTTATCATGATACT
  GGCAATTTAAACTTTAGTTTTATAACAGATGATGATGGTAACAGTTTCCACAATTTCCAA
  AAATTCACTGAACCAATTGTTCAAGCATTACAATCTTTAGGCGTAAATGCTGAATTAACT
  GGTCGTAACGATATTCAAGTTGGACAAGCGAAAATCTCAGGGAATGCTATGGTTAAAGTT
  AAAAATAGAATGTTTAGTCATGGTACGTTGATGTTAAATAGTGATTTAGACGAAGTTCAG
  AATGCACTAAAAGTTAATCCAGCTAAGATTAAATCAAAAGGTATTAAATCTGTTCGTAAA
  CGGGTAGCAAACATTCAAGAATTTTTAAATGACCCTTTAGAAATTGAAGAATTTAAAAAA
  ATCATCTTGAAAACTATCTTTGGAGAAACTGAAGTAGAAGAATATAAATTAACGGATGAA
  GATTGGGAAAATATTGAAAAGTTAAGTAACGACAAATATAGAACTTGGGAATGGAATTAT
  GGTAGAAACCCTAAATATAACTTTGAACGCGAGGAAAAATTTGAAAAAGGATTTGTACAA
  ATTAAGTTTGATGTTAAACGAGGTAAAATCGAACATGCAAAAATATTCGGTGATTTCTTT
  GGTGTCGGAGATGTCACTGATCTTGAAAATGCATTAGTTGGCTGTCTACACGACTTTGAG
  CACATAGAAGAAGCACTATCAGAATATGATTTATATCATTATTTCGGTGACATAGATAGA
  CATGAATTAATTAGATTAATGTCATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0930 [new locus tag: SAUSA300_RS04995 ]
• symbol: SAUSA300_0930
• description: lipoate-protein ligase A family protein
• length: 328
• theoretical pI: 4.91531
• theoretical MW: 37927.4
• GRAVY: -0.522256

⊟Function

• reaction:
  EC 6.3.4.-?  ExPASy
  EC 6.3.1.20?  ExPASy
  Lipoate--protein ligaseATP + (R)-lipoate + a [lipoyl-carrier protein]-L-lysine = a [lipoyl-carrier protein]-N⁶-(lipoyl)lysine + AMP + diphosphate
• TIGRFAM:
  Genetic information processingProtein fateProtein modification and repairlipoyltransferase and lipoate-protein ligase (TIGR00545; HMM-score: 385.6)
• TheSEED:  
  Amino Acids and DerivativesAlanine, serine, and glycineGlycine cleavage system Lipoate-protein ligase A 
  and 2 more
  CarbohydratesCentral carbohydrate metabolismDehydrogenase complexes Lipoate-protein ligase A 
  Cofactors, Vitamins, Prosthetic Groups, PigmentsLipoic acidLipoic acid metabolism Lipoate-protein ligase A 
• PFAM:
  SufE_NifU (CL0233) Lip_prot_lig_C; Bacterial lipoate protein ligase C-terminus (PF10437; HMM-score: 94.4)
  and 2 more
  tRNA_synt_II (CL0040) BPL_LplA_LipB; Biotin/lipoate A/B protein ligase family (PF03099; HMM-score: 36)
  AB_hydrolase (CL0028) DUF726; Protein of unknown function (DUF726) (PF05277; HMM-score: 13.5)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular Possibility: 1
  • Signal Peptide Possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.142
  • Ymax_pos: 18
  • Cmax: 0.137
  • Cmax_pos: 18
  • Smax: 0.189
  • Smax_pos: 15
  • Smean: 0.138
  • D: 0.141
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160642 NCBI
• RefSeq: YP_493628 NCBI
• UniProt: A0A0H2XFJ1 UniProt

⊟Protein sequence

• MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIVGKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKFTEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQNALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLTDEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIFGDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIDRHELIRLMS

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• SAUSA300_0930 no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Other Information

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