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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1334 [new locus tag: SAUSA300_RS07270 ]
  • pan locus tag?: SAUPAN003897000
  • symbol: SAUSA300_1334
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_1334 [new locus tag: SAUSA300_RS07270 ]
  • symbol: SAUSA300_1334
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1498745..1500085
  • length: 1341
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAAAATACATTTAATCCATTACAAAGATTACATTTCTATGCAGCAATATTTATTGCT
    CCACTGTTAATCACTTTAACCATTTCGGGCATTGGTTACTTGTTCTTTCCAGAAGTTGAA
    AATAATATTTATAAGAATGAGTTTTTTGGTGACAGTGATGTAAAAACGCATCAAACATTA
    AATGATGCAGTACATCAAGTTGAACAACAATATGAAGGATTCTTTGTAAGTAAAGTTAGC
    ATACTTGATGAACCATATAACAAACGAATTACACTGAGTGATATGGCAGGAAATCAACGT
    TACGTCTTTCTAGATCATAACAATCAATTTGTTGCAGATCAAAATGCGAAACATACGTAT
    TCTAATGTGATGCGAAGTATACATAGTTCTTTGTTTACTGAAAATACTATTATTAATTAT
    TTAGTAGAGTTAACCGCATGTTGGATGATATTCATGATTTTATCTGGTACTTATTTACTC
    ATTAAGAAGCATTTAATTTCTAACAAAAGTAAGGCACTTCGTTGGCAAAAGTGGCACGCA
    ATGATTGGAGTTATCATTGCAATTCCAGTATTTGTATTAGTCTTAACTGGATTGCCATGG
    TCTGGTTTTATGGGCAGTAAAATTGCCGGTATGATGGACACAAACGGTGCCCTTGGTCAA
    GGTGAATTAGCGATTAATCCACCTAAATCAGATTTGAACGAATTACCTTGGGCTACACGT
    AAAAATAAACAGCCAGCTTCATCCGAAAAAGGTTCAAGTGGTCATCATGGTAATGCAGCA
    ATGCCTCAAACCAAATTAGATTATCAAATATCTATTGATAAGGTCGTTGAACAGGCGCAA
    AAAGCTGGTATTAAAAAGCCGTTTTCAATCGTATATCCAAGTGATAAAAATGGTACCTTT
    ATTGTATCTAATACTAGTAATTCAGGTGTTACTGGGCTAGATGTATCACCATACAAGGAA
    CAAACACTTTATTTCGATCAATATAGCGGTAAAAAGCTAGGTACGATTAAATATGATGAC
    TACGGTATTATTGCTAAATGGTTTACATGGGGCATTCCGCTTCACGAAGGTCATTTATTC
    GGCATTTTAAATAAAATCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGC
    ATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGC
    GTAAAAAAACCAGCATCTATATCACTCATAATATGTTTAATTGTATTAGGATTATTAATG
    CCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAA
    GATCGTCGTGCTAAACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1341

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_1334 [new locus tag: SAUSA300_RS07270 ]
  • symbol: SAUSA300_1334
  • description: hypothetical protein
  • length: 446
  • theoretical pI: 9.82061
  • theoretical MW: 50171.3
  • GRAVY: 0.0874439

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    TIGR03943 family protein (TIGR03943; HMM-score: 9.9)
  • TheSEED:  
    Iron-uptake factor PiuB 
    Uncharacterized iron-regulated membrane protein 
  • PFAM:
    PepSY_TM-like (CL0490) PepSY_TM; PepSY-associated TM region (PF03929; HMM-score: 177.1)
    no clan definedSIT; SHP2-interacting transmembrane adaptor protein, SIT (PF15330; HMM-score: 12.6)
    Peptidase_A8; Signal peptidase (SPase) II (PF01252; HMM-score: 9.7)
    TctB; Tripartite tricarboxylate transporter TctB family (PF07331; HMM-score: 8.6)
    DUF1980; Domain of unknown function (DUF1980) (PF09323; HMM-score: 7.2)
    Cytochrom_B_C; Cytochrome b(C-terminal)/b6/petD (PF00032; HMM-score: 7.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.171
    • Ymax_pos: 14
    • Cmax: 0.113
    • Cmax_pos: 31
    • Smax: 0.356
    • Smax_pos: 9
    • Smean: 0.283
    • D: 0.215
  • predicted transmembrane helices (TMHMM): 5

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIYKNEFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYVFLDHNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWMIFMILSGTYLLIKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVLTGLPWSGFMGSKIAGMMDTNGALGQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVVEQAQKAGIKKPFSIVYPSDKNGTFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLGTIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVAIGMGFVSWIKRTKNTAIKVPHRVKKPASISLIICLIVLGLLMPLFGLSLILVFIIELILYIKDRRAKQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAUSA300_1334 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]