NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1370 [new locus tag: SAUSA300_RS07485 ]
• pan locus tag^?: SAUPAN003946000
• symbol: ebpS
• pan gene symbol^?: ebpS
• synonym:
• product: cell surface elastin binding protein

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1370 [new locus tag: SAUSA300_RS07485 ]
• symbol: ebpS
• product: cell surface elastin binding protein
• replicon: chromosome
• strand: -
• coordinates: 1537066..1538526
• length: 1461
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913412 NCBI
• RefSeq: YP_494067 NCBI
• MicrobesOnline: 1292885 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
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  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  
  ATGTCTAATAATTTTAAAGATGACTTTGAAAAAAATCGTCAATCGATAGACACAAATTCA
  CATCAAGACCATACGGAAGATGTTGAAAAAGACCAATCAGAATTAGAACATCAGGATACA
  ATAGAGAATACGGAGCAACAGTTTCCGCCAAGAAATGCCCAAAGAAGAAAAAGACGCCGT
  GATTTAGCAACGAATCATAATAAACAAGTTCACAATGAATCACAAACATCTGAAGACAAT
  GTTCAAAATGAGGCTGGCACAATAGATGATCGTCAAGTCGAATCATCACACAGTACTGAA
  AGTCAAGAACCTAGCCATCAAGACAGTACACCTCAACATGAAGAGGAATATTATAATAAG
  AATGCTTTTGCAATGGATAAATCACATCCAGAACCAATCGAAGACAATGATAAACACGAT
  ACTATTAAAAATGCAGAAAATAACACTGAGCATTCAACAGTTTCTGATAAGAGTGAAGCT
  GAACAATCTCAGCAACCTAAACCATATTTTACAACAGGTGCTAACCAATCAGAAACATCA
  AAAAATGAACATGATAATGATTCTGTAAAACAAGATCAAGATGAACCTAAAGAACATCAT
  AATGGTAAAAAAGCAGCAGCTATTGGTGCTGGAACAGCAGGTGTTGCAGGTGCAGCTGGT
  GCAATGGCTGCTTCTAAAGCTAAGAAACATTCAAATGACGCTCAAAACAAAAGTAATTCT
  GGCAAGGCGAATAACTCGACTGAGGATAAAGCGTCTCAAGATAAGTCTAAAGATCATCAT
  AATGGCAAAAAAGGTGCAGCGATCGGTGCTGGAACAGCAGGTTTGGCTGGAGGCGCAGCA
  AGTAAAAGTGCTTCTGCCGCTTCAAAACCACATGCCTCTAATAATGCAAGCCAAAACCAT
  GATGAACATGACAATCATGACAGAGATAAAGAACGTAAAAAAGGTGGCATGGCCAAAGTA
  TTGTTACCATTAATTGCAGCTGTACTAATTATCGGTGCATTAGCGATATTTGGAGGCATG
  GCATTAAACAATCATAATAATGGTACAAAAGAAAATAAAATCGCGAATACAAATAAAAAT
  AATGCTGATGAAAGTAAAGACAAAGACACATCTAAAGACGCTTCTAAAGATAAATCAAAA
  TCTACAGACAGTGATAAATCAAAAGAGGATCAAGACAAAGCGACTAAAGATGAATCTGAT
  AATGATCAAAACAACGCTAATCAAGCGAACAATCAAGCACAAAATAATCAAAATCAACAA
  CAAGCTAATCAAAATCAACAACAGCAACAACAACGTCAAGGTGGTGGCCAAAGACATACA
  GTGAATGGTCAAGAAAACTTATACCGTATCGCAATTCAATACTACGGTTCAGGTTCACCG
  GAAAATGTTGAAAAAATTAGACGTGCCAATGGTTTAAGTGGTAACAATATTAGAAACGGT
  CAACAAATCGTTATTCCATAA
  60
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  1260
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  1440
  1461
  

⊟Protein

⊟General

• locus tag: SAUSA300_1370 [new locus tag: SAUSA300_RS07485 ]
• symbol: EbpS
• description: cell surface elastin binding protein
• length: 486
• theoretical pI: 6.26146
• theoretical MW: 53221
• GRAVY: -1.557

⊟Function

• TIGRFAM:
• TheSEED:  
  Virulence, Disease and DefenseAdhesionAdhesins in Staphylococcus Elastin binding protein EbpS 
• PFAM:
  LysM (CL0187) LysM; LysM domain (PF01476; HMM-score: 25.1)
  and 2 more
  no clan definedDUF1510; Protein of unknown function (DUF1510) (PF07423; HMM-score: 19.5)
  Tetraspannin (CL0347) DUF4064; Protein of unknown function (DUF4064) (PF13273; HMM-score: 13.7)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.01
  • Cytoplasmic Membrane Score: 8.28
  • Cellwall Score: 1.71
  • Extracellular Score: 0.01
  • Internal Helix: 1
• LocateP: Intracellular /TMH start AFTER 60
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: Possibly Sec-
  • Intracellular Possibility: 0.17
  • Signal Peptide Possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.126
  • Ymax_pos: 11
  • Cmax: 0.108
  • Cmax_pos: 23
  • Smax: 0.221
  • Smax_pos: 1
  • Smean: 0.143
  • D: 0.133
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 87160749 NCBI
• RefSeq: YP_494067 NCBI
• UniProt: Q2FGW1 UniProt

⊟Protein sequence

• MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENTEQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQEPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHDTIKNAENNTEHSTVSDKSEAEQSQQPKPYFTTGANQSETSKNEHDNDSVKQDQDEPKEHHNGKKAAAIGAGTAGVAGAAGAMAASKAKKHSNDAQNKSNSGKANNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLAGGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGALAIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQDKATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRIAIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: ebpS no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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