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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1452 [new locus tag: SAUSA300_RS07930 ]
  • pan locus tag?: SAUPAN004036000
  • symbol: proC
  • pan gene symbol?: proC
  • synonym:
  • product: pyrroline-5-carboxylate reductase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1452 [new locus tag: SAUSA300_RS07930 ]
  • symbol: proC
  • product: pyrroline-5-carboxylate reductase
  • replicon: chromosome
  • strand: +
  • coordinates: 1604865..1605650
  • length: 786
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCACAAGCTATATTTACAGGAATTATTAACTCAAGCAACTTAGATGCCAATGATATA
    TATTTAACAAATAAATCTAATGAACAAGCTTTAAAAGCATTCGCTGAAAAACTAGGTGTT
    AACTATAGTTATGATGATGCGACATTATTAAAAGATGCAGATTATGTATTTTTAGGTACC
    AAACCACATGATTTTGATGCTCTAGCAACACGCATCAAACCACATATTACAAAAGACAAT
    TGCTTCATTTCAATTATGGCAGGTATTCCGATTGATTATATTAAACAACAATTAGAATGC
    CAAAATCCAGTTGCTAGAATTATGCCAAACACAAATGCGCAAGTTGGACACTCTGTTACT
    GGCATTAGTTTTTCAAACAACTTTGACCCTAAATCTAAAGATGAAATTAACGATTTAGTT
    AAAGCATTTGGTTCTGTAATTGAAGTATCAGAAGATCATTTACATCAAGTAACAGCTATC
    ACCGGAAGCGGCCCAGCATTTTTATATCATGTATTCGAGCAATATGTTAAAGCTGGTACG
    AAACTTGGTCTAGAAAAAGAACAAGTTGAAGAATCTATACGCAACCTTATTATAGGTACA
    AGTAAGATGATTGAACGTTCAGATTTGAGCATGGCTCAATTAAGAAAAAATATTACCTCT
    AAAGGTGGTACGACACAAGCTGGCCTTGATACATTGTCACAATATGATTTAGTATCTATT
    TTCGAAGATTGTCTAAACGCTGCCGTCGACCGTAGTATTGAACTTTCTAATATAGAAGAC
    CAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    786

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1452 [new locus tag: SAUSA300_RS07930 ]
  • symbol: ProC
  • description: pyrroline-5-carboxylate reductase
  • length: 261
  • theoretical pI: 4.67633
  • theoretical MW: 28758.2
  • GRAVY: -0.228352

Function[edit | edit source]

  • reaction:
    EC 1.5.1.2?  ExPASy
    Pyrroline-5-carboxylate reductase L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family pyrroline-5-carboxylate reductase (TIGR00112; EC 1.5.1.2; HMM-score: 272.2)
  • TheSEED  :
    • Pyrroline-5-carboxylate reductase (EC 1.5.1.2)
    Amino Acids and Derivatives Proline and 4-hydroxyproline A Hypothetical Protein Related to Proline Metabolism  Pyrroline-5-carboxylate reductase (EC 1.5.1.2)
    and 2 more
    Amino Acids and Derivatives Proline and 4-hydroxyproline Proline Synthesis  Pyrroline-5-carboxylate reductase (EC 1.5.1.2)
    Amino Acids and Derivatives Proline and 4-hydroxyproline Proline, 4-hydroxyproline uptake and utilization  Pyrroline-5-carboxylate reductase (EC 1.5.1.2)
  • PFAM:
    6PGD_C (CL0106) P5CR_dimer; Pyrroline-5-carboxylate reductase dimerisation (PF14748; HMM-score: 94.8)
    and 5 more
    NADP_Rossmann (CL0063) F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 47.7)
    PDH; Prephenate dehydrogenase (PF02153; HMM-score: 16.9)
    no clan defined DUF4918; Domain of unknown function (DUF4918) (PF16265; HMM-score: 15)
    NADP_Rossmann (CL0063) NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 14.6)
    ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 12.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009616
    • TAT(Tat/SPI): 0.000268
    • LIPO(Sec/SPII): 0.000925
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MAQAIFTGIINSSNLDANDIYLTNKSNEQALKAFAEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPIDYIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVSEDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSDLSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]