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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1725 [new locus tag: SAUSA300_RS09425 ]
  • pan locus tag?: SAUPAN004479000
  • symbol: SAUSA300_1725
  • pan gene symbol?: tal
  • synonym:
  • product: putative translaldolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_1725 [new locus tag: SAUSA300_RS09425 ]
  • symbol: SAUSA300_1725
  • product: putative translaldolase
  • replicon: chromosome
  • strand: -
  • coordinates: 1907471..1908184
  • length: 714
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGCTAAACTAAATATAGAAGTATTTGCGGACGGTGCAGATATTGAAGAAATGAAAGCA
    GCTTATAAAAACAAACAAGTGGATGGTTTTACAACAAATCCTAGTTTAATGGCTAAAGCG
    GGCGTAACAGATTACAAAGCTTTTGCTGAAGAAGCTGTGAAAGAAATTCCAGATGCTTCA
    ATTTCATTTGAAGTATTTGCAGACGATTTAGAAACTATGGAAAAAGAAGCAGCAATTTTA
    AAACAATATGGCGAAAATGTATTTGTTAAAATTCCTATTGTAAATACAAAAGGTGAATCA
    ACGATTCCTTTAATTAAAAAACTTTCAGCTGACAATGTGAGATTAAACGTTACGGCTGTT
    TACACAATTGAACAAGTTAAAGAAATAACTGAAGCAGTAACTGAAGGTGTGCCAACATAT
    GTTTCAGTATTTGCAGGACGTATTGCAGATACAGGCGTAGATCCATTACCATTAATGAAA
    GAGGCTGTAAAAGTTACGCATAGTAAAGACGGCGTTAAATTATTATGGGCAAGTTGCCGC
    GAATTATTCAATGTGATTCAAGCTGATGAAATTGGTGCAGATATTATTACATGCCCAGCA
    GATGTTGTGAAAAAAGTGAATACAAACTTAGGTCGCGATATTAACGAATTATCAGTAGAT
    ACAGTTAAAGGCTTTGCGAAAGATATTCAATCTTCAGGTCTTTCTATTCTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    714

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_1725 [new locus tag: SAUSA300_RS09425 ]
  • symbol: SAUSA300_1725
  • description: putative translaldolase
  • length: 237
  • theoretical pI: 4.45164
  • theoretical MW: 25719.3
  • GRAVY: 0.00632911

Function[edit source | edit]

  • reaction:
    EC 2.2.1.2?  ExPASy
    TransaldolaseSedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + D-fructose 6-phosphate
  • TIGRFAM:
    MetabolismEnergy metabolismPentose phosphate pathwaytransaldolase (TIGR02134; EC 2.2.1.2; HMM-score: 442)
    and 3 more
    MetabolismEnergy metabolismPentose phosphate pathwayfructose-6-phosphate aldolase (TIGR00875; EC 4.1.2.-; HMM-score: 102.1)
    MetabolismEnergy metabolismPentose phosphate pathwaytransaldolase (TIGR00874; EC 2.2.1.2; HMM-score: 24.1)
    MetabolismEnergy metabolismPentose phosphate pathwaytransaldolase (TIGR00876; EC 2.2.1.2; HMM-score: 22.1)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismPentose phosphate pathway Transaldolase (EC 2.2.1.2) 
    and 1 more
    MonosaccharidesFructose utilization Transaldolase (EC 2.2.1.2) 
  • PFAM:
    TIM_barrel (CL0036) TAL_FSA; Transaldolase/Fructose-6-phosphate aldolase (PF00923; HMM-score: 130)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SAUSA300_0996(lpdA)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    SAUSA300_0993(pdhA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    SAUSA300_0524(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    SAUSA300_1134(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    SAUSA300_1603(rplU)50S ribosomal protein L21  [1] (data from MRSA252)
    SAUSA300_2198(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    SAUSA300_2179(rpsK)30S ribosomal protein S11  [1] (data from MRSA252)
    SAUSA300_0995branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.145
    • Ymax_pos: 50
    • Cmax: 0.182
    • Cmax_pos: 50
    • Smax: 0.191
    • Smax_pos: 37
    • Smean: 0.089
    • D: 0.124
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAKLNIEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTDYKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTIPLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMKEAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELSVDTVKGFAKDIQSSGLSIL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAUSA300_1725 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]