From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2198 [new locus tag: SAUSA300_RS12120 ]
  • pan locus tag?: SAUPAN005696000
  • symbol: rpsC
  • pan gene symbol?: rpsC
  • synonym:
  • product: 30S ribosomal protein S3

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2198 [new locus tag: SAUSA300_RS12120 ]
  • symbol: rpsC
  • product: 30S ribosomal protein S3
  • replicon: chromosome
  • strand: -
  • coordinates: 2367160..2367813
  • length: 654
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    GTGGGTCAAAAAATTAATCCAATCGGACTTCGTGTTGGTATTATCCGTGATTGGGAAGCT
    AAATGGTATGCTGAAAAAGACTTCGCTTCACTTTTACACGAAGATTTAAAAATCCGTAAA
    TTTATTGATAATGAATTAAAAGAAGCATCAGTTTCTCACGTAGAGATTGAACGTGCTGCA
    AACCGTATCAACATTGCAATTCATACTGGTAAACCTGGTATGGTAATTGGTAAAGGCGGT
    TCAGAAATCGAAAAATTACGCAACAAATTAAATGCGTTAACTGATAAAAAAGTACACATC
    AACGTAATTGAAATCAAAAAAGTTGATCTTGACGCTCGTTTAGTAGCTGAAAACATCGCA
    CGTCAATTAGAAAACCGTGCTTCATTCCGTCGTGTACAAAAACAAGCAATCACTAGAGCT
    ATGAAACTTGGTGCTAAAGGTATCAAAACTCAAGTATCTGGTCGTTTAGGCGGAGCTGAC
    ATCGCTCGTGCTGAACAATATTCAGAAGGAACTGTTCCACTTCATACGTTACGTGCTGAC
    ATCGATTATGCACACGCTGAAGCTGACACTACTTACGGTAAATTAGGCGTTAAAGTATGG
    ATTTATCGTGGAGAAGTTCTTCCTACTAAGAACACTAGTGGAGGAGGAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    654

Protein[edit | edit source]

Protein Data Bank: 5LI0
Protein Data Bank: 5ND8
Protein Data Bank: 5ND9
Protein Data Bank: 5TCU

General[edit | edit source]

  • locus tag: SAUSA300_2198 [new locus tag: SAUSA300_RS12120 ]
  • symbol: RpsC
  • description: 30S ribosomal protein S3
  • length: 217
  • theoretical pI: 10.4222
  • theoretical MW: 24099.6
  • GRAVY: -0.428111

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein uS3 (TIGR01009; HMM-score: 312.7)
    and 3 more
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein uS3 (TIGR01008; HMM-score: 113.8)
    NusA family KH domain protein, archaeal (TIGR01952; HMM-score: 20.1)
    Genetic information processing Transcription Degradation of RNA polyribonucleotide nucleotidyltransferase (TIGR03591; EC 2.7.7.8; HMM-score: 14.6)
  • TheSEED  :
    • SSU ribosomal protein S3p (S3e)
    Protein Metabolism Protein biosynthesis Ribosome SSU bacterial  SSU ribosomal protein S3p (S3e)
  • PFAM:
    no clan defined Ribosomal_S3_C; Ribosomal protein S3, C-terminal domain (PF00189; HMM-score: 131.2)
    and 5 more
    KH (CL0007) KH_2; KH domain (PF07650; HMM-score: 74.2)
    KH_4; KH domain (PF13083; HMM-score: 20.2)
    KH_5; NusA-like KH domain (PF13184; HMM-score: 19.3)
    KH_1; KH domain (PF00013; HMM-score: 12.9)
    no clan defined SpoOE-like; Spo0E like sporulation regulatory protein (PF09388; HMM-score: 12.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002317
    • TAT(Tat/SPI): 0.000116
    • LIPO(Sec/SPII): 0.000356
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDNELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINVIEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGADIARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK

Experimental data[edit | edit source]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 1.49 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]