From AureoWiki
Jump to: navigation, search
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2489 [new locus tag: SAUSA300_RS13825 ]
  • pan locus tag?: SAUPAN006214000
  • symbol: SAUSA300_2489
  • pan gene symbol?:
  • synonym:
  • product: antibiotic transport-associated protein-like protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_2489 [new locus tag: SAUSA300_RS13825 ]
  • symbol: SAUSA300_2489
  • product: antibiotic transport-associated protein-like protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2690001..2692469
  • length: 2469
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGGGACATTCATCGCAAAACATAAATGGTCCGCGGTCATTGCATGGATAGTGATTGTA
    GCTGCAATATTGATACCACTTGCTACAAATGCACCGAAATTTGACAATGATATTAAAATG
    ACAGGATTAGAGTCGTTAGATACGAATAAAAAGATTGAAAAACATTTTAATCAAGATAGT
    GAAAAGGCACAAATACGTGTTGTCTTTAAGACGACTAAAGATGATGGTATTGTGCAACCG
    AACATTACTAAAGATATTAAGAAAACGTTAGATGATATTAAAAAAGATGACAAGCACATT
    GATAAAATATCAGATCCTTATGAAAATAAGCAAATTAGTAAAGATAAAACAACTGCATTT
    GCAGATATCACATATGACGTTAGTCAAACATCTTTAAAAGATGGCTCTAGAGATAATGTT
    AAAAGTCATTTGAAAGACTTACGTGACAATCATAATGTACAAACAGAGTTAACTGGTACA
    GGAATGACATCTACAGAAGTAGGCGGTAATTCTGAACTAGTTGGTATTATTGTTGCATTT
    GTTGTACTGTTAATCACATTCGGTTCAGTCATTGCTGCAGGATTACCAATTATAAGTGCA
    TTGATTGGTCTAGCCTCTGGTGTAGGTATTATCAGTTTGTTAACTTATGCATTCGATATA
    CCAAATGTCACACTGACATTAGCCGTAATGATTGGACTAGCAGTTGGTATCGATTATGCA
    TTATTTATTTTATTCAGATATCGACAAGTTATGAAAACTGAAACAGATTACATTAAAGGC
    ATTGGTCTTGCAATAGGAACAGCAGGTAGTGCAGTTGTTTTTGCTGGTGTAACAGTTGTA
    ATTGCAGTATGTGGTTTATCATTAGTAGGCATTGACTTCTTAGCGGTAATGGGATTTGCT
    TCGGCAATTAGTGTTGTTTTTGCAGTACTTAGCGCTTTAACTTTATTACCTGCATTAATA
    AGCATTTTCCATAAACGCATTAAAGTGAATAAATTACAGTCTAAATTCAAAAAAGATATT
    GATACGCCATGGTCTAAATTTATTACAGGAAATGCACTTGCGGCAGTATTACTAGGTTTA
    ATCATATTAGTAGCAGCTGCAATACCAGTTAGTCATATGCGACTTGGTATTCCAGATGAC
    GGTGTTAAACCAGCAGATTCTACACAGAAAAAAGCTTACGATATTATCTCGGATAAATTT
    GGTGAAGGATTTAATGGCCAAATACCGATGTTGATAAATGTGAAAGATAAAAAGGATGAT
    CCACAAGGATTACAACAAGATTTACAGTCTGTATATAAAGATATTAAAGATAAGAAAAAT
    GTAGATATCGTTACGCCACCACAAATGAGTAAAGATAATGATTACGCTTTGATGGTCGTG
    ATACCTAAGCAAGGGCCTAATGCAGAGAGTACAAATGATTTAGTGCATGATTTACGAGAT
    TATCATAAAGATGCACAAGATAAATATGGGTTCAAAACGGAGATTTCGGGACAAAGTGTC
    ATTAATATTGATATGTCTAAAAAGTTAAATGAGGCGATACCTTTATTTGCCTCAGTCATT
    GTTGTATTAGCATTTTTCTTATTAATGATTGTCTTCCGTTCAATTCTAATACCATTAAAA
    GCAGTTTTAGGATTCGTCTTGTCATTAATGGCAACATTAGGATTTACAACATTAGTAATG
    CAAGATGGTTTTATGAAAGGTCTGTTTGGTATTGAAACGACAGGACCAATGCTAGCATTT
    TTACCGGTTATTACTATAGGTATCTTATTTGGATTAGCGATGGATTATGAAGTCTTCCTT
    ATGTCGCGAATTCATGAAGAATATAGTAAAACAGGGGATAATGATTATTCTATCAAAGTA
    GGATTGAAAGAAAGCGGTCCTGTTATTGTAGCAGCTGCATTAATTATGTTTAGTGTCTTC
    TTTGCGTTTGTATTCCAAGAAGATGTCATGATTAAATCAATGGGTATGGCATTAGCGTTT
    GGTGTACTCTTTGATGCCTTCGTAGTCAGAATGATGTTAATTCCTGCATTAACGAAATTG
    TTTGGAAAAGGTTCGTGGTATTTACCAGCATGGTTGAATCGCATTATTCCACGAGTTGAT
    ATTGAAGGACATGCACTTGAAAAATATAAAACGGTTGAATCACAGGAATCTGAAGCTAAA
    GATAGTAAGGAAACATACGACACAACCTTTAAAGTATATCCACAAGGTGCAACAAATGTA
    TCGAAACATCAAGATGCACATGGGCAAGATGATGCACATTCAATAGTACTAGATGATAAA
    ACGATGGCATTGTACCAAGAAGTGAAACAGCAATCAGCAAGTTCATTGTTCTTATATGAT
    GCTTTAATAGATTATCAAAACAAGCATCAACTAAATTCAAAAAAACAAGCAACGAATATA
    GAACAGTTGAATAAAAATATTGAAAAGCTCAATCAATTGTTAGAAAAAAATCTACGAAAT
    AAATCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2469

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_2489 [new locus tag: SAUSA300_RS13825 ]
  • symbol: SAUSA300_2489
  • description: antibiotic transport-associated protein-like protein
  • length: 822
  • theoretical pI: 7.09563
  • theoretical MW: 90408.7
  • GRAVY: 0.158273

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsUnknown substratetransport protein (TIGR00833; HMM-score: 225.2)
    MetabolismTransport and binding proteinsUnknown substrateefflux transporter, putative, hydrophobe/amphiphile efflux-3 (HAE3) family (TIGR00921; HMM-score: 121.3)
    hopanoid biosynthesis associated RND transporter like protein HpnN (TIGR03480; HMM-score: 60.9)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingprotein-export membrane protein, SecD/SecF family (TIGR00916; HMM-score: 8.8)
    transmembrane receptor Patched (TIGR00918; HMM-score: 5)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingprotein-export membrane protein SecD (TIGR01129; HMM-score: 4.3)
  • TheSEED:  
    CBSS-196620.1.peg.2477 Putative antibiotic transport-associated protein 
  • PFAM:
    RND_permease (CL0322) MMPL; MMPL family (PF03176; HMM-score: 358.2)
    no clan definedPAP_fibrillin; PAP_fibrillin (PF04755; HMM-score: 11.8)
    Fez1; Fez1 (PF06818; HMM-score: 8.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.322
    • Ymax_pos: 31
    • Cmax: 0.2
    • Cmax_pos: 31
    • Smax: 0.638
    • Smax_pos: 21
    • Smean: 0.42
    • D: 0.36
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDNDIKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKKDDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNVQTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGIISLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYIKGIGLAIGTAGSAVVFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVVFAVLSALTLLPALISIFHKRIKVNKLQSKFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPADSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDIVTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHKDAQDKYGFKTEISGQSVINIDMSKKLNEAIPLFASVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLVMQDGFMKGLFGIETTGPMLAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYSIKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIPALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVYPQGATNVSKHQDAHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQLNSKKQATNIEQLNKNIEKLNQLLEKNLRNKS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]