NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS00105 [old locus tag: SAUSA300_0020 ]
• pan locus tag^?: SAUPAN000032000
• symbol: SAUSA300_RS00105
• pan gene symbol^?: walR
• synonym:
• product: DNA-binding response regulator

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS00105 [old locus tag: SAUSA300_0020 ]
• symbol: SAUSA300_RS00105
• product: DNA-binding response regulator
• replicon: chromosome
• strand: +
• coordinates: 24952..25653
• length: 702
• essential: unknown

⊟Accession numbers

• Location: NC_007793 (24952..25653) NCBI
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
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  301
  361
  421
  481
  541
  601
  661
  
  ATGGCTAGAAAAGTTGTTGTAGTTGATGATGAAAAACCGATTGCTGATATTTTAGAATTT
  AACTTAAAAAAAGAAGGATACGATGTGTACTGTGCATACGATGGTAATGATGCAGTCGAC
  TTAATTTATGAAGAAGAACCAGACATCGTATTACTAGATATCATGTTACCTGGTCGTGAT
  GGTATGGAAGTATGTCGTGAAGTGCGCAAAAAATACGAAATGCCAATAATAATGCTTACT
  GCTAAAGATTCAGAAATTGATAAAGTGCTTGGTTTAGAACTAGGTGCAGATGACTATGTA
  ACGAAACCGTTTAGTACGCGTGAATTAATCGCACGTGTGAAAGCGAACTTACGTCGTCAT
  TACTCACAACCAGCACAAGACACTGGAAATGTAACGAATGAAATCACAATTAAAGATATT
  GTGATTTATCCAGACGCATATTCTATTAAAAAACGTGGCGAAGATATTGAATTAACACAT
  CGTGAATTTGAATTGTTCCATTATTTATCAAAACATATGGGACAAGTAATGACACGTGAA
  CATTTATTACAAACAGTATGGGGCTATGATTACTTTGGCGATGTACGTACGGTCGATGTA
  ACGATTCGTCGTTTACGTGAAAAGATTGAAGATGATCCGTCACATCCTGAATATATTGTG
  ACGCGTAGAGGCGTTGGATATTTCCTCCAACAACATGAGTAG
  60
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  420
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  702
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS00105 [old locus tag: SAUSA300_0020 ]
• symbol: SAUSA300_RS00105
• description: DNA-binding response regulator
• length: 233
• theoretical pI: 4.81211
• theoretical MW: 27191.8
• GRAVY: -0.475536

⊟Function

• reaction:
• TIGRFAM:
  Signal transductionRegulatory functionsDNA interactionsphosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 235.8)
  Signal transductionSignal transductionTwo-component systemsphosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 235.8)
  Signal transductionRegulatory functionsDNA interactionsheavy metal response regulator (TIGR01387; HMM-score: 191.9)
  and 7 more
  proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 132.5)
  MetabolismCentral intermediary metabolismNitrogen metabolismnitrogen regulation protein NR(I) (TIGR01818; HMM-score: 70.6)
  Signal transductionRegulatory functionsDNA interactionsnitrogen regulation protein NR(I) (TIGR01818; HMM-score: 70.6)
  Signal transductionSignal transductionTwo-component systemsnitrogen regulation protein NR(I) (TIGR01818; HMM-score: 70.6)
  Cellular processesCellular processesSporulation and germinationsporulation transcription factor Spo0A (TIGR02875; HMM-score: 69.4)
  Signal transductionSignal transductionTwo-component systemsTMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 41.1)
  Signal transductionRegulatory functionsDNA interactionsPEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 40.3)
• TheSEED:
• PFAM:
  CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 103.1)
  HTH (CL0123) Trans_reg_C; Transcriptional regulatory protein, C terminal (PF00486; HMM-score: 97)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

  SAUSA300_RS02340		ABC transporter substrate-binding protein  [1] (data from MRSA252)
  SAUSA300_RS02635		cysteine synthase  [1] (data from MRSA252)
  SAUSA300_RS05360		dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex  [1] (data from MRSA252)
  SAUSA300_RS06220		30S ribosomal protein S2  [1] (data from MRSA252)
  SAUSA300_RS07445		30S ribosomal protein S1  [1] (data from MRSA252)
  SAUSA300_RS11380		uracil phosphoribosyltransferase  [1] (data from MRSA252)
  SAUSA300_RS12120		30S ribosomal protein S3  [1] (data from MRSA252)
  SAUSA300_RS14105		malate:quinone oxidoreductase  [1] (data from MRSA252)

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • Ymax: 0.099
  • Ymax_pos: 70
  • Cmax: 0.104
  • Cmax_pos: 33
  • Smax: 0.112
  • Smax_pos: 68
  • Smean: 0.065
  • D: 0.086
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446024121 NCBI
• RefSeq: WP_000101976 NCBI
• UniProt:

⊟Protein sequence

• MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYEEEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTKPFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTHREFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEYIVTRRGVGYFLQQHE

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• SAUSA300_RS00105 no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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