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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS06475 [old locus tag: SAUSA300_1199 ]
  • pan locus tag?: SAUPAN003624000
  • symbol: SAUSA300_RS06475
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS06475 [old locus tag: SAUSA300_1199 ]
  • symbol: SAUSA300_RS06475
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1320910..1322148
  • length: 1239
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAAAGATATAAGTAAGATAGTAGCTGACGTCGAATCAACGTTAGCACCATATTTTAAA
    GAAATTGAAGAAACAGCATATATTAATCAAGAAAAAGTATTAAATGCATTTCATCATGTC
    AAAGCAACCGAAAGTGATCTACAAGGATCAACAGGATACGGGTATGATGACTTTGGACGT
    GATCATTTAGAAGAAATATATGCGCAGGCATTTAAAGCAGAAGATGCAATTGTTCGTCCG
    CAAATTATTTCAGGTACGCATGCGATTACTATTGCATTACAAAGTTTATTAAAACATGGT
    GATGAATTAATTTATATAACGGGTAGTCCATATGACACTTTACTTGAAGTCATTGGCGTA
    AACGGAAATGGTATTGAAAGTTTAATGGAGCATGGCGTATCGTATAAAGATATTGCACTT
    AAAGAAGGTAAGATCGATATTGAAAGTGTGTTAGATGGGGTTTCTGAGCGCACCAAAGTA
    ATAGCGATTCAACGTTCGAAAGGCTATGATCAAAGACCTTCAATTCCGCTAGATGAAATT
    GAAAAGGTAATTACTAGGTTGAAAAACGTGCATCCTAATATTTTAATATTTGTGGATAAC
    TGTTATGGGGAATTTGTTGAAAGACGTGAACCTATAGAATGTGGTGCCGATTTAATAGCA
    GGATCATTAATTAAAAACCCTGGCGGTGGTTTAGCTAAGATTGGTGGATACATTGCTGGT
    AGAAAAGATTTAATTGAACGATGTGGTTATAGATTGACAGCACCTGGTATTGGTAAAGAA
    GCGGGTGCATCATTAAATGCATTGCTTGAAATGTATCAAGGTTTCTTTTTAGCACCACAC
    GTTGTCAGTCAGAGTCTTAAAGGTGCATTGTTTACTAGTTTATTTTTAGAAAAAATGAAT
    ATGAACACAACGCCGAAGTACTACGAAAAACGAACTGATTTAATTCAAACAGTTAAATTT
    GAAACGAAAGAACAAATGATTTCATTTTGTCAAAGTATTCAACACGCATCCCCAATTAAT
    GCACATTTTAGTCCAGAACCTAGTTATATGCCTGGTTACGAAGATGATGTTATTATGGCA
    GCTGGTACGTTTATTCAAGGTTCATCGATTGAATTATCTGCAGATGGACCTATTCGTCCT
    CCTTATGAAGCATATGTTCAAGGAGGATTAACATATGAACACGTTAAAATTGCTGTGACA
    AGAGCTGTTAATCAGTTGAAAGAACAAGGACTTATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1239

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS06475 [old locus tag: SAUSA300_1199 ]
  • symbol: SAUSA300_RS06475
  • description: hypothetical protein
  • length: 412
  • theoretical pI: 5.01316
  • theoretical MW: 45487.5
  • GRAVY: -0.179854

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines methionine gamma-lyase (TIGR01328; EC 4.4.1.11; HMM-score: 29.8)
    and 4 more
    Metabolism Amino acid biosynthesis Aspartate family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 22.8)
    Metabolism Amino acid biosynthesis Serine family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 22.8)
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine sulfhydrylase (TIGR01325; EC 4.2.99.-; HMM-score: 18)
    Metabolism Amino acid biosynthesis Aspartate family cystathionine beta-lyase (TIGR01324; EC 4.4.1.8; HMM-score: 12.9)
  • TheSEED: see SAUSA300_1199
  • PFAM:
    PLP_aminotran (CL0061) Met_gamma_lyase; Methionine gamma-lyase (PF06838; HMM-score: 653.8)
    and 3 more
    Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 25)
    Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 23.4)
    Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 12.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007992
    • TAT(Tat/SPI): 0.000317
    • LIPO(Sec/SPII): 0.000522
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATESDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDELIYITGSPYDTLLEVIGVNGNGIESLMEHGVSYKDIALKEGKIDIESVLDGVSERTKVIAIQRSKGYDQRPSIPLDEIEKVITRLKNVHPNILIFVDNCYGEFVERREPIECGADLIAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFFLAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFETKEQMISFCQSIQHASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTYEHVKIAVTRAVNQLKEQGLI

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]