From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS07545 [old locus tag: SAUSA300_1382 ]
  • pan locus tag?: SAUPAN000661000
  • symbol: SAUSA300_RS07545
  • pan gene symbol?: lukS-PV
  • synonym:
  • product: gamma-hemolysin subunit A

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS07545 [old locus tag: SAUSA300_1382 ]
  • symbol: SAUSA300_RS07545
  • product: gamma-hemolysin subunit A
  • replicon: chromosome
  • strand: -
  • coordinates: 1547149..1548087
  • length: 939
  • essential: unknown

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGTCAAAAAAAGACTATTAGCTGCAACATTGTCGTTAGGAATAATCACTCCTATTGCT
    ACTTCGTTTCATGAATCTAAAGCTGATAACAATATTGAGAATATTGGTGATGGCGCTGAG
    GTAGTCAAAAGAACAGAAGATACAAGTAGCGATAAGTGGGGGGTCACACAAAATATTCAG
    TTTGATTTTGTTAAAGATAAAAAGTATAACAAAGACGCTTTGATTTTAAAAATGCAAGGT
    TTTATCAATTCAAAGACTACTTATTACAATTACAAAAACACAGATCATATAAAAGCAATG
    AGGTGGCCTTTCCAATACAATATTGGTCTCAAAACAAATGACCCCAATGTAGATTTAATA
    AATTATCTACCTAAAAATAAAATAGATTCAGTAAATGTTAGTCAAACATTAGGTTATAAC
    ATAGGTGGTAATTTTAATAGTGGTCCATCAACAGGAGGTAATGGTTCATTTAATTATTCA
    AAAACAATTAGTTATAATCAACAAAACTATATCAGTGAAGTAGAACGTCAAAATTCAAAA
    AGTGTTCAATGGGGAATAAAAGCTAATTCATTTATCACATCATTAGGTAAAATGTCTGGA
    CATGATCCAAATTTATTTGTTGGATATAAACCATATAGTCAAAATCCGAGAGACTATTTT
    GTTCCAGACAATGAATTACCCCCATTAGTACACAGTGGTTTCAATCCTTCATTTATTGCA
    ACTGTTTCTCATGAAAAAGGCTCAGGAGATACAAGTGAATTTGAAATAACGTATGGCAGA
    AATATGGATGTTACTCATGCTACTAGAAGAACAACACACTATGGCAATAGTTATTTAGAA
    GGATCTAGAATACACAACGCATTTGTAAACAGAAATTACACAGTTAAATATGAAGTGAAC
    TGGAAAACTCATGAAATTAAAGTGAAAGGACATAATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    939

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS07545 [old locus tag: SAUSA300_1382 ]
  • symbol: SAUSA300_RS07545
  • description: gamma-hemolysin subunit A
  • length: 312
  • theoretical pI: 9.61932
  • theoretical MW: 35313.1
  • GRAVY: -0.729487

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance beta-channel forming cytolysin (TIGR01002; HMM-score: 286.9)
  • TheSEED: see SAUSA300_1382
  • PFAM:
    Leukocidin (CL0636) Leukocidin; Leukocidin/Hemolysin toxin family (PF07968; HMM-score: 275.1)
    and 1 more
    MBB (CL0193) YadA_anchor; YadA-like membrane anchor domain (PF03895; HMM-score: 8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 28 aa
    • SP(Sec/SPI): 0.979556
    • TAT(Tat/SPI): 0.004869
    • LIPO(Sec/SPII): 0.006074
    • Cleavage Site: CS pos: 28-29. SKA-DN. Pr: 0.9414
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MVKKRLLAATLSLGIITPIATSFHESKADNNIENIGDGAEVVKRTEDTSSDKWGVTQNIQFDFVKDKKYNKDALILKMQGFINSKTTYYNYKNTDHIKAMRWPFQYNIGLKTNDPNVDLINYLPKNKIDSVNVSQTLGYNIGGNFNSGPSTGGNGSFNYSKTISYNQQNYISEVERQNSKSVQWGIKANSFITSLGKMSGHDPNLFVGYKPYSQNPRDYFVPDNELPPLVHSGFNPSFIATVSHEKGSGDTSEFEITYGRNMDVTHATRRTTHYGNSYLEGSRIHNAFVNRNYTVKYEVNWKTHEIKVKGHN

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]