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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40

NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1382 [new locus tag: SAUSA300_RS07545 ]
  • pan locus tag?: SAUPAN000661000
  • symbol: lukS-PV
  • pan gene symbol?: lukS-PV
  • synonym:
  • product: Panton-Valentine leukocidin, LukS-PV

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_1382 [new locus tag: SAUSA300_RS07545 ]
  • symbol: lukS-PV
  • product: Panton-Valentine leukocidin, LukS-PV
  • replicon: chromosome
  • strand: -
  • coordinates: 1547149..1548087
  • length: 939
  • essential: unknown

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGTCAAAAAAAGACTATTAGCTGCAACATTGTCGTTAGGAATAATCACTCCTATTGCT
    ACTTCGTTTCATGAATCTAAAGCTGATAACAATATTGAGAATATTGGTGATGGCGCTGAG
    GTAGTCAAAAGAACAGAAGATACAAGTAGCGATAAGTGGGGGGTCACACAAAATATTCAG
    TTTGATTTTGTTAAAGATAAAAAGTATAACAAAGACGCTTTGATTTTAAAAATGCAAGGT
    TTTATCAATTCAAAGACTACTTATTACAATTACAAAAACACAGATCATATAAAAGCAATG
    AGGTGGCCTTTCCAATACAATATTGGTCTCAAAACAAATGACCCCAATGTAGATTTAATA
    AATTATCTACCTAAAAATAAAATAGATTCAGTAAATGTTAGTCAAACATTAGGTTATAAC
    ATAGGTGGTAATTTTAATAGTGGTCCATCAACAGGAGGTAATGGTTCATTTAATTATTCA
    AAAACAATTAGTTATAATCAACAAAACTATATCAGTGAAGTAGAACGTCAAAATTCAAAA
    AGTGTTCAATGGGGAATAAAAGCTAATTCATTTATCACATCATTAGGTAAAATGTCTGGA
    CATGATCCAAATTTATTTGTTGGATATAAACCATATAGTCAAAATCCGAGAGACTATTTT
    GTTCCAGACAATGAATTACCCCCATTAGTACACAGTGGTTTCAATCCTTCATTTATTGCA
    ACTGTTTCTCATGAAAAAGGCTCAGGAGATACAAGTGAATTTGAAATAACGTATGGCAGA
    AATATGGATGTTACTCATGCTACTAGAAGAACAACACACTATGGCAATAGTTATTTAGAA
    GGATCTAGAATACACAACGCATTTGTAAACAGAAATTACACAGTTAAATATGAAGTGAAC
    TGGAAAACTCATGAAATTAAAGTGAAAGGACATAATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    939

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_1382 [new locus tag: SAUSA300_RS07545 ]
  • symbol: LukS-PV
  • description: Panton-Valentine leukocidin, LukS-PV
  • length: 312
  • theoretical pI: 9.61932
  • theoretical MW: 35313.1
  • GRAVY: -0.729487

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesToxin production and resistancebeta-channel forming cytolysin (TIGR01002; HMM-score: 286.9)
  • TheSEED:  
    Virulence, Disease and DefenseToxins and superantigensStaphylococcus Two-component and Pore-forming Cytolysins Panton-Valentine leukocidin chain S precursor 
  • PFAM:
    Leukocidin (CL0636) Leukocidin; Leukocidin/Hemolysin toxin family (PF07968; HMM-score: 275.1)
    MBB (CL0193) YadA_anchor; YadA-like membrane anchor domain (PF03895; HMM-score: 8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: -0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide length 29 aa
    • Ymax: 0.561
    • Ymax_pos: 29
    • Cmax: 0.445
    • Cmax_pos: 29
    • Smax: 0.953
    • Smax_pos: 19
    • Smean: 0.779
    • D: 0.646
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MVKKRLLAATLSLGIITPIATSFHESKADNNIENIGDGAEVVKRTEDTSSDKWGVTQNIQFDFVKDKKYNKDALILKMQGFINSKTTYYNYKNTDHIKAMRWPFQYNIGLKTNDPNVDLINYLPKNKIDSVNVSQTLGYNIGGNFNSGPSTGGNGSFNYSKTISYNQQNYISEVERQNSKSVQWGIKANSFITSLGKMSGHDPNLFVGYKPYSQNPRDYFVPDNELPPLVHSGFNPSFIATVSHEKGSGDTSEFEITYGRNMDVTHATRRTTHYGNSYLEGSRIHNAFVNRNYTVKYEVNWKTHEIKVKGHN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SaeR
    SaeR (TF) important in Virulence  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]