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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS07615 [old locus tag: SAUSA300_1396 ]
  • pan locus tag?: SAUPAN001825000
  • symbol: SAUSA300_RS07615
  • pan gene symbol?:
  • synonym:
  • product: tail protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS07615 [old locus tag: SAUSA300_1396 ]
  • symbol: SAUSA300_RS07615
  • product: tail protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1564191..1564646
  • length: 456
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGACTAAAACTTTAAAGGTTTATAAAGGAGACGACGTCGTAGCTTCTGAACAAGGTGAA
    GGCAAAGTGTCAGTAACTTTATCTAATTTAGAAGCGGATACAACTTATCCAAAAGGTACT
    TACCAAGTGGCATGGGAAGAAAATGGTAAAGAATCTAGTAAAGTTGATGTACCTCAATTC
    AAAACCAATCCAATTCTAGTCTCAGGCGTATCATTTACACCAGAAACTAAATCAATTATG
    GTAAATACCGATGACAATGTTGAGCCAAACATTGCACCAAGCACAGCAACGAATAAAATA
    TTGAAATATACAAGTGAACATCCAGAATTTGTTACTGTAGATGAAAATACAGGAGCAATT
    CACGGTGTAGCTGAAGGTACTTCAGTAATCACTGCTATGTCTACTGATGGAAGCGATAAG
    TCAGGACAAATTTCAGTGACAGTAACAAACGGATAG
    60
    120
    180
    240
    300
    360
    420
    456

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS07615 [old locus tag: SAUSA300_1396 ]
  • symbol: SAUSA300_RS07615
  • description: tail protein
  • length: 151
  • theoretical pI: 4.33262
  • theoretical MW: 16032.6
  • GRAVY: -0.435099

Function[edit | edit source]

  • TIGRFAM:
    isopropylmalate/isohomocitrate dehydrogenases (TIGR02088; HMM-score: 12.6)
    streptococcal pilin isopeptide linkage domain (TIGR03786; HMM-score: 11)
  • TheSEED: see SAUSA300_1396
  • PFAM:
    E-set (CL0159) Big_2; Bacterial Ig-like domain (group 2) (PF02368; HMM-score: 36.2)
    and 2 more
    Pur_ac_phosph_N; Purple acid Phosphatase, N-terminal domain (PF16656; HMM-score: 12.9)
    SprB; SprB repeat (PF13573; HMM-score: 8.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.025925
    • TAT(Tat/SPI): 0.00154
    • LIPO(Sec/SPII): 0.005576
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTKTLKVYKGDDVVASEQGEGKVSVTLSNLEADTTYPKGTYQVAWEENGKESSKVDVPQFKTNPILVSGVSFTPETKSIMVNTDDNVEPNIAPSTATNKILKYTSEHPEFVTVDENTGAIHGVAEGTSVITAMSTDGSDKSGQISVTVTNG

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAUSA300_RS05350pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    SAUSA300_RS05360dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex  [1] (data from MRSA252)

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. 1.0 1.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]