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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS08035 [old locus tag: SAUSA300_1472 ]
  • pan locus tag?: SAUPAN004078000
  • symbol: SAUSA300_RS08035
  • pan gene symbol?: xseA
  • synonym:
  • product: exodeoxyribonuclease 7 large subunit

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS08035 [old locus tag: SAUSA300_1472 ]
  • symbol: SAUSA300_RS08035
  • product: exodeoxyribonuclease 7 large subunit
  • replicon: chromosome
  • strand: -
  • coordinates: 1626588..1627925
  • length: 1338
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTCAGATTATTTAAGTGTTTCAGCTTTAACGAAATATATTAAATATAAATTTGATCAA
    GATCCACATCTCCAATCTGTATTGATTAAAGGTGAGCTTTCAAATTTCAAAAAGCATTCA
    AGTGGTCATTTATACTTTAATGTGAAAGATAAAGAAAGCGTTATAAGTGCCATGATGTTC
    AAAGGTAGTGCTTCTAAATTAAACTTCGAACCTAAAGAAGGAGATGAAGTCTTATTAGAA
    GCACGTGTTTCTGTTTTTGAACGTCGTGGAAACTATCAAATTTATGTAAATAAAATGCAA
    TTAGATGGTATAGGGAACTTATATCAAAAATTAGAAGCATTAAAGAAAAAATTAACTGAA
    GAAGGTTGTTTTGATAAAGCGAATAAGAAATCAATACCTAAGTTTCCTAAAAAAATTGCA
    GTTTTAACAGCGAGTACAGGTGCCGCAATTCGAGATATCCATTCAACGATAAATAGTCGT
    TTTCCATTAGCTGAACAAATACAAATTAGTACTTTGGTTCAAGGTGAAAAAGCAAAAGAC
    GACATTATTGAAAAAATTGAATATGCAGATAGTTTAGGTGTAGATACCATTATTGTAGGT
    CGAGGCGGTGGATCCATAGAAGATTTATGGAATTTCAACGAAGAAGCTGTCGTCCGTGCA
    ATATATAATTGTAAGACACCGATTATATCAGCAGTTGGTCATGAAACAGACTTTACATTA
    AGTGATTTTGCTGCAGACATCAGAGCTGCGACTCCAACTCAAGCTGCTGTTATTGCAACA
    CCAGACCAATATGAATTGCTGCAACAAATACAGCAATATCAGTTTACATTGACTCGTTTC
    ATCAAGAAACATTTAGAACAACAACGTAAACATGTTGAACATTTGTCATCGTATTACAAG
    TTTAAACAACCAACTTTATTGTATGATCAACAGATACAACGTCGAGATGATTTAGAAAAG
    AGACTGAAACAACAAATTCAGGCAACTTTTGAACAACAGAGACATCGATTAATGCTGTTG
    CAACAACGTTATAATTTAAAAGCATTACTAAGTAGTGTTAATCAAGAACAACAAAATAAT
    CTTCAATTGACAAACCAACTTGTTAAATTATTGAATTCTAAGATATTAAGTTATAAAAAT
    GATTTGAAAAATAAAGTAGAAAACTTAAACAATTTAAGTCCAACTAATACAATGTTGCGT
    GGATATGCAATTGTTAATAAAAAAGACGAAGTCATTACGAGTACTAAAGATTTAACAGAA
    AATGATCAATTGACATTAACAATGAAAGATGGCTTAGTAGATGCAAAAGTTACGAAAGTA
    AGGTGTAATAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1338

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS08035 [old locus tag: SAUSA300_1472 ]
  • symbol: SAUSA300_RS08035
  • description: exodeoxyribonuclease 7 large subunit
  • length: 445
  • theoretical pI: 9.48419
  • theoretical MW: 50893.8
  • GRAVY: -0.52809

Function[edit | edit source]

  • reaction:
    EC 3.1.11.6?  ExPASy
    Exodeoxyribonuclease VII Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates
  • TIGRFAM:
    Genetic information processing DNA metabolism Degradation of DNA exodeoxyribonuclease VII, large subunit (TIGR00237; EC 3.1.11.6; HMM-score: 489)
  • TheSEED: see SAUSA300_1472
  • PFAM:
    no clan defined Exonuc_VII_L; Exonuclease VII, large subunit (PF02601; HMM-score: 311.8)
    and 7 more
    OB (CL0021) tRNA_anti_2; OB-fold nucleic acid binding domain (PF13742; HMM-score: 96.8)
    tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 34.4)
    Cache (CL0165) dCache_1; Cache domain (PF02743; HMM-score: 16.6)
    TIM_barrel (CL0036) AP_endonuc_2; Xylose isomerase-like TIM barrel (PF01261; HMM-score: 12.3)
    PepSY_TM-like (CL0490) PepSY_TM_like_2; Putative PepSY_TM-like (PF16357; HMM-score: 11.8)
    no clan defined Transglut_i_TM; Inactive transglutaminase fused to 7 transmembrane helices (PF14400; HMM-score: 11.3)
    DUF4279; Domain of unknown function (DUF4279) (PF14106; HMM-score: 10.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002793
    • TAT(Tat/SPI): 0.000164
    • LIPO(Sec/SPII): 0.000268
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHLYFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLDGIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSRFPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVVRAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFTLTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQRHRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNLSPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]