NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS09600 [old locus tag: SAUSA300_1754 ]
• pan locus tag^?: SAUPAN004561000
• symbol: SAUSA300_RS09600
• pan gene symbol^?: splE
• synonym:
• product: serine protease SplE

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS09600 [old locus tag: SAUSA300_1754 ]
• symbol: SAUSA300_RS09600
• product: serine protease SplE
• replicon: chromosome
• strand: -
• coordinates: 1939625..1940341
• length: 717
• essential: unknown

⊟Accession numbers

• Location: NC_007793 (1939625..1940341) NCBI
• MicrobesOnline:

⊟Phenotype

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⊟DNA sequence

• 1
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  661
  
  ATGAATAAAAATATAATCATCAAAAGTATTGCAGCATTGACGATTTTAACATCAGTGACT
  GGCGTCGGCACAACAGTGGTTGAGGGTATTCAACAAACGGCTAAAGCTGAACATAATGTG
  AAACTAATCAAAAATACTAATGTAGCACCATACAATGGTGTCGTTTCGATAGGATCTGGA
  ACAGGTTTCATTGTCGGTAAAAATACAATTGTTACCAACAAGCATGTCGTTGCAGGTATG
  GAAATTGGTGCACATATTATAGCGCATCCCAATGGTGAATATAATAATGGCGGATTTTAT
  AAAGTTAAAAAAATTGTCCGTTATTCAGGTCAAGAAGATATTGCCATTCTACATGTGGAA
  GATAAAGCTGTTCATCCAAAAAACAGGAATTTTAAAGATTACACAGGCATTTTAAAAATA
  GCATCAGAAGCTAAAGAAAATGAACGCATTTCAATTGTTGGCTATCCAGAACCATATATA
  AATAAATTTCAAATGTATGAGTCAACAGGAAAAGTGCTGTCAGTTAAAGGCAACATGATT
  ATTACTGATGCTTTCGTAGAACCAGGCAACTCAGGTTCAGCTGTATTTAACAGTAAATAC
  GAAGTTGTAGGTGTTCACTTTGGTGGAAACGGCCCTGGAAATAAAAGTACAAAAGGATAT
  GGTGTTTATTTCTCTCCTGAAATTAAGAAATTCATTGCAGATAACACAGATAAATAA
  60
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  717
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS09600 [old locus tag: SAUSA300_1754 ]
• symbol: SAUSA300_RS09600
• description: serine protease SplE
• length: 238
• theoretical pI: 9.81932
• theoretical MW: 25679.1
• GRAVY: -0.195378

⊟Function

• reaction:
  EC 3.4.21.19?  ExPASy
  Glutamyl endopeptidasePreferential cleavage: Glu-|-Xaa, Asp-|-Xaa
• TIGRFAM:
  Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidespeptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 35)
  Genetic information processingProtein fateProtein folding and stabilizationpeptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 35)
  Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesperiplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 24.8)
  Signal transductionRegulatory functionsProtein interactionsperiplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 24.8)
• TheSEED:
• PFAM:
  Peptidase_PA (CL0124) Trypsin; Trypsin (PF00089; HMM-score: 71.6)
  Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 70.6)
  Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 24.6)
  Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 24.6)
  Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 18.5)
  DUF31; Putative peptidase (DUF31) (PF01732; HMM-score: 15.9)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.09
  • Cellwall Score: 0.18
  • Extracellular Score: 9.73
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide length 37 aa
  • Ymax: 0.561
  • Ymax_pos: 37
  • Cmax: 0.536
  • Cmax_pos: 37
  • Smax: 0.864
  • Smax_pos: 11
  • Smean: 0.706
  • D: 0.617
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 446961503 NCBI
• RefSeq: WP_001038759 NCBI
• UniProt:

⊟Protein sequence

• MNKNIIIKSIAALTILTSVTGVGTTVVEGIQQTAKAEHNVKLIKNTNVAPYNGVVSIGSGTGFIVGKNTIVTNKHVVAGMEIGAHIIAHPNGEYNNGGFYKVKKIVRYSGQEDIAILHVEDKAVHPKNRNFKDYTGILKIASEAKENERISIVGYPEPYINKFQMYESTGKVLSVKGNMIITDAFVEPGNSGSAVFNSKYEVVGVHFGGNGPGNKSTKGYGVYFSPEIKKFIADNTDK

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• SAUSA300_RS09600 no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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