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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS14175 [old locus tag: SAUSA300_2551 ]
  • pan locus tag?: SAUPAN006322000
  • symbol: SAUSA300_RS14175
  • pan gene symbol?: nrdD
  • synonym:
  • product: anaerobic ribonucleoside-triphosphate reductase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_RS14175 [old locus tag: SAUSA300_2551 ]
  • symbol: SAUSA300_RS14175
  • product: anaerobic ribonucleoside-triphosphate reductase
  • replicon: chromosome
  • strand: -
  • coordinates: 2757385..2759235
  • length: 1851
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_007793 (2757385..2759235) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAATCAAATCGAAGAAGCATTAACGGGTTTGATTTCTAAAGATCCTGCTATTGTTAAC
    GAAAATGCTAACAAAGATAGTGATACATTTTCAACAATGAGAGATTTAACAGCAGGTATC
    GTTTCTAAATCTTACGCATTAAATCATTTATTACCAAAGCACGTTGCAGATGCACATCAA
    AGAGGGGACATACATTTTCACGACTTAGATTATCATCCATTCCAACCGTTAACTAACTGT
    TGTTTAATAGATGCTAAAAATATGCTACATAATGGATTTGAAATAGGCAACGCGAATGTA
    ACTTCACCAAAATCAATACAAACTGCATCAGCGCAGCTTGTACAAATTATAGCCAATGTT
    TCTAGCAGTCAATATGGTGGCTGTACGGTTGACCGCGTTGACGAATTACTTAGTACATAT
    GCACGACATAATGAAGAACAACATAGGAATATCGCAAAGCAATTTGTCAAAGAATCTGAA
    ATTGATCGTTATGTTGATCAACAAGTCACTAAAGACATCAATGATGCGATTGAAAGTTTA
    GAATATGAAATTAATACCTTATATACATCTAATGGACAGACACCTTTTGTAACATTAGGA
    TTCGGCTTAGGTACAGATCATTTAAGTCGCAAAATTCAACAAGCTATCTTAAATACTCGT
    ATCAAAGGCTTAGGAAAAGACCGCACGACAGCGATTTTCCCGAAACTTGTATTTTCAATT
    AAAAAAGGAACCAACTTTAGTCCGCAAGATCCGAACTATGACATTAAACAACTAGCATTA
    AAGTGTTCAACGAAACGTATGTATCCAGATATTTTAAATTATGACAAACTCGTAGAAATA
    TTAGGTGATTTCAAAGCGCCAATGGGTTGTCGTTCATTTTTACCAAGTTGGAAAGATGCG
    GAAGGTCATTTTGAAAATAATGGTCGTTGTAATCTTGGTGTTGTTACACTTAATTTACCT
    AGAATGGCATTAGAATCTGCCGGTAATATGACGAAATTCTGGGAAATCTTTTATGAACGT
    ATCGATGTGTTACATGATGCATTACTTTATCGTATAAATCGTTTGAAAGATGCTGTACCG
    AATAACGCACCGATTTTATATAAAAGTGGCGCATTTAACTATAAATTAAAAGAAACAGAT
    GATGTTGCTGAGTTATTTAAAAATAAACGTGCAACGATTTCAATGGGCTATATAGGGTTG
    TATGAAACAGCTACTGTTTTCTATGGTCCAGACTGGGAAACATCTCAAGAAGCAAAAGCA
    TTTACGCTTGAAATTCTTAAAGAAATGAAACGTTATCAAACGAAATGGACAGAATTATAT
    GACATTTGGTTCAGTATTTACAGTACGCCGAGTGAATCGCTAACGGATCGTTTTTGTCGT
    TTAGACCAAGAGAGATTTGGAGATATTAAAGACATTACAGATAAAGGATATTATCAAAAC
    TCTTTCCATTATGATGTACGTAAAGATGTTACACCTTTTGAAAAGTTAGATTTTGAAAAA
    GATTATCCTTATTATGCGAGTGGTGGTTTCATTCACTATTGTGAGTATCCGAAATTGCAA
    CACAATTTGAAAGCACTAGAAGCGGTATGGGACTACTCTTATGACAAAGTTGGTTACTTA
    GGTACAAATATTCCGATTGATCATTGTTATGAATGTGATTACGATGGAGATTTTGAAGCA
    ACTGAAAAAGGATTTAAATGCCCGAACTGTGGCAATGATAATCCTAAAACAGTTGATGTC
    GTTAAACGAACATGTGGTTACCTAGGCAATCCAGTTCAACGTCCAGTAATTAAAGGCCGT
    CATAAAGAAATTTGCGCACGAGTAAAACATATGAAAGCGCCTAAAGAATGA
    60
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    480
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    1020
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    1140
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    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1851

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_RS14175 [old locus tag: SAUSA300_2551 ]
  • symbol: SAUSA300_RS14175
  • description: anaerobic ribonucleoside-triphosphate reductase
  • length: 616
  • theoretical pI: 6.50952
  • theoretical MW: 70479.2
  • GRAVY: -0.541396

Function[edit source | edit]

  • reaction:
    EC 1.17.4.2?  ExPASy
    Ribonucleoside-triphosphate reductase2'-deoxyribonucleoside triphosphate + thioredoxin disulfide + H2O = ribonucleoside triphosphate + thioredoxin
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotides2'-Deoxyribonucleotide metabolismanaerobic ribonucleoside-triphosphate reductase (TIGR02487; EC 1.17.4.2; HMM-score: 576.5)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotides2'-Deoxyribonucleotide metabolismanaerobic ribonucleoside-triphosphate reductase (TIGR02827; EC 1.17.4.2; HMM-score: 64.4)
    Genetic information processingTranscriptionTranscription factorstranscription factor E (TIGR00373; HMM-score: 13.5)
    Genetic information processingProtein fateProtein folding and stabilizationchaperone protein DnaK (TIGR02350; HMM-score: 10.6)
  • TheSEED:
  • PFAM:
    PFL-like (CL0339) NRDD; Anaerobic ribonucleoside-triphosphate reductase (PF13597; HMM-score: 589.7)
    Gly_radical; Glycine radical (PF01228; HMM-score: 88.9)
    Phosphatase (CL0031) NleF_casp_inhib; NleF caspase inhibitor (PF16809; HMM-score: 17)
    Zn_Beta_Ribbon (CL0167) zinc_ribbon_2; zinc-ribbon domain (PF13240; HMM-score: 13.1)
    zf-FPG_IleRS; Zinc finger found in FPG and IleRS (PF06827; HMM-score: 13)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.149
    • Ymax_pos: 59
    • Cmax: 0.173
    • Cmax_pos: 26
    • Smax: 0.24
    • Smax_pos: 54
    • Smean: 0.106
    • D: 0.132
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446994461 NCBI
  • RefSeq: WP_001071717 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKSYALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTSPKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESEIDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILNTRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDKLVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFWEIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRATISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTPSESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASGGFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFKCPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAUSA300_RS14175 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]