COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 23-MAY-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_pUSA030028 [new locus tag: SAUSA300_RS14875 ]
• pan locus tag^?:
• symbol: SAUSA300_pUSA030028
• pan gene symbol^?: —
• synonym:
• product: resolvase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_pUSA030028 [new locus tag: SAUSA300_RS14875 ]
• symbol: SAUSA300_pUSA030028
• product: resolvase
• replicon: pUSA03
• strand: +
• coordinates: 26735..27367
• length: 633
• essential: unknown

⊟Accession numbers

• Gene ID: 3912728 NCBI
• RefSeq: YP_492714 NCBI
• BioCyc: GH3C-2662 BioCyc
• MicrobesOnline: 1291507 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  TTGAGAATTAATTTTATAGATAACTATACATTAAAAACAATATACAACGCTATTTTAGAA
  AGGATAATTACAATTATGGCCAATATTGGTTATGCTCGTGTATCTACCAGAGATCAAAGT
  TTAGATTTACAAATAGACGCTTTGAGTAATTTTGGTTGTGAGAAAATATTTAGCGAAAAA
  GTTAGTGGGCGTAAAGTAAATAGAACTGAACTAGATAAATGTTTAGATTATTTACGTGAA
  GGCGACACTTTAGTTATTTACAAGCTAGATAGATTAGGCAGGACTACTAAGCAACTGATA
  GATTTAGCCCAATGGTTAGAAAATAATAATATTGAATTACAAATTATTGATATGAATATC
  AATACAAAAGACCCAATGGGAAAGATGTTTTTTACAATGATGAGTGCTTTTGCTGAATTA
  GAGGCTAACTTATTAAGCGAAAGAACTAAAAAAGGTTTAGAATCAGCTAGAGCAAGAGGT
  AACATGGGAGGCAGAAAAGCAATGCCACAAGAAAAAAAAGAATACATTAAACATTTATAC
  GATACCAAAGAATATACAGGACAAGAAATTGCTAAAAAAACGAATGTAAGTAGAGATACT
  GTATATCGCATTTTACGAGAGTTTAAAAAGTAA
  60
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  633
  

⊟Protein

⊟General

• locus tag: SAUSA300_pUSA030028 [new locus tag: SAUSA300_RS14875 ]
• symbol: SAUSA300_pUSA030028
• description: resolvase
• length: 210
• theoretical pI: 9.71682
• theoretical MW: 24442.1
• GRAVY: -0.534762

⊟Function

• TIGRFAM:
  RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 20.4)
  Unknown function General DNA binding domain, excisionase family (TIGR01764; HMM-score: 19.8)
  and 3 more
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage-associated protein, BcepMu gp16 family (TIGR04111; HMM-score: 13.9)
  RNA polymerase sigma-70 factor, Bacteroides expansion family 1 (TIGR02985; HMM-score: 12.9)
  transcriptional regulator BotR, P-21 (TIGR03209; HMM-score: 11.8)
• TheSEED  :
  • Resolvase/integrase
• PFAM:
  no clan defined Resolvase; Resolvase, N terminal domain (PF00239; HMM-score: 150.8)
  and 26 more
  HTH (CL0123) HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 33)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 26.9)
  HTH_11; HTH domain (PF08279; HMM-score: 25.7)
  HTH_29; Winged helix-turn helix (PF13551; HMM-score: 24.4)
  HTH_32; Homeodomain-like domain (PF13565; HMM-score: 23)
  TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 19.5)
  HTH_22; HTH domain (PF13309; HMM-score: 19.4)
  HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 19.3)
  Mga; Mga helix-turn-helix domain (PF05043; HMM-score: 18.6)
  HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 16.9)
  UPF0122; Putative helix-turn-helix protein, YlxM / p13 like (PF04297; HMM-score: 16.8)
  HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 16.6)
  Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 16.4)
  LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 16.1)
  no clan defined DUF4277; Domain of unknown function (DUF4277) (PF14104; HMM-score: 15.5)
  HTH (CL0123) HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 15)
  HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 14.4)
  CENP-B_N; CENP-B N-terminal DNA-binding domain (PF04218; HMM-score: 14.3)
  HTH_10; HTH DNA binding domain (PF04967; HMM-score: 14.1)
  Sigma70_r4_2; Sigma-70, region 4 (PF08281; HMM-score: 13.4)
  HTH_17; Helix-turn-helix domain (PF12728; HMM-score: 13)
  HTH_3; Helix-turn-helix (PF01381; HMM-score: 12.8)
  Rrf2; Transcriptional regulator (PF02082; HMM-score: 12.8)
  no clan defined TnsD; Tn7-like transposition protein D (PF15978; HMM-score: 12.7)
  HTH (CL0123) TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 12.4)
  HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.033789
  • TAT(Tat/SPI): 0.001704
  • LIPO(Sec/SPII): 0.002289
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159861 NCBI
• RefSeq: YP_492714 NCBI
• UniProt: A0A0H2XJH5 UniProt

⊟Protein sequence

• MRINFIDNYTLKTIYNAILERIITIMANIGYARVSTRDQSLDLQIDALSNFGCEKIFSEKVSGRKVNRTELDKCLDYLREGDTLVIYKLDRLGRTTKQLIDLAQWLENNNIELQIIDMNINTKDPMGKMFFTMMSAFAELEANLLSERTKKGLESARARGNMGGRKAMPQEKKEYIKHLYDTKEYTGQEIAKKTNVSRDTVYRILREFKK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications