NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS00780 [old locus tag: SA0123 ]
• pan locus tag^?: SAUPAN000935000
• symbol: SA_RS00780
• pan gene symbol^?: galE
• synonym:
• product: NAD-dependent dehydratase

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS00780 [old locus tag: SA0123 ]
• symbol: SA_RS00780
• product: NAD-dependent dehydratase
• replicon: chromosome
• strand: +
• coordinates: 142135..143115
• length: 981
• essential: unknown

⊟Accession numbers

• Location: NC_002745 (142135..143115) NCBI
• MicrobesOnline:

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
  181
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  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  
  GTGGAAGATTTGGAAAGAGTTTTGATAACTGGTGGGGCTGGTTTTATTGGGTCGCATTTA
  GTAGATGATTTACAACAAGATTATGATGTTTATGTTCTAGATAACTATAGAACAGGTAAA
  CGAGAAAATATTAAAAGTTTGGCTGACGATCATGTGTTTGAATTAGATATTCGTGAATAT
  GATGCAGTTGAACAAATCATGAAGACATATCAATTTGATTATGTTATTCATTTAGCAGCA
  TTAGTTAGTGTTGCTGAGTCGGTTGAGAAACCTATCTTATCTCAAGAAATAAACGTCGTA
  GCAACATTAAGATTGTTAGAAATCATTAAAAAATATAATAGTCATATAAAACGTTTTATC
  TTTGCTTCGTCAGCAGCTGTTTATGGTGATCTTCCTGATTTGCCTAAAAGTGATCAATCA
  TTAATCTTACCATTATCACCATATGCAATAGATAAATATTACGGCGAACGGACGACATTA
  AATTATTGCTCGTTATATAACATACCAACAGCGGTTGTTAAATTTTTTAATGTATTTGGG
  CCAAGACAGGATCCTAAGTCACAATATTCAGGTGTTATTTCAAAGATGTTCGATTCATTT
  GAGCATAACAAGCCATTTACATTTTTTGGTGACGGACTGCAAACTAGAGATTTTGTATAT
  GTATATGATGTTGTTCAATCTGTACGCTTAATTATGGAACACAAAGATGCAATTGGACAC
  GGTTATAACATTGGTACAGGCACTTTTACTAATTTATTAGAGGTTTATCGTATTATTGGT
  GAATTATATGGAAAATCAGTCGAGCATGAATTTAAAGAAGCACGAAAAGGAGATATTAAG
  CATTCTTATGCAGATATTTCTAACTTAAAGGCATTAGGATTTGTTCCTAAATATACAGTA
  GAAACAGGTTTAAAGGATTACTTTAATTTTGAGGTAGATAATATTGAAGAAGTTACAGCT
  AAAGAAGTGGAAATGTCGTGA
  60
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  360
  420
  480
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  981
  

⊟Protein

⊟General

• locus tag: SA_RS00780 [old locus tag: SA0123 ]
• symbol: SA_RS00780
• description: NAD-dependent dehydratase
• length: 326
• theoretical pI: 4.85005
• theoretical MW: 37256.9
• GRAVY: -0.210123

⊟Function

• reaction:
• TIGRFAM:
  MetabolismEnergy metabolismSugarsUDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 171.3)
  Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesdTDP-glucose 4,6-dehydratase (TIGR01181; EC 4.2.1.46; HMM-score: 170.4)
  Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesADP-glyceromanno-heptose 6-epimerase (TIGR02197; EC 5.1.3.20; HMM-score: 153.3)
  NAD dependent epimerase/dehydratase, LLPSF_EDH_00030 family (TIGR04180; HMM-score: 134.3)
  Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesGDP-mannose 4,6-dehydratase (TIGR01472; EC 4.2.1.47; HMM-score: 65.1)
  Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesCDP-glucose 4,6-dehydratase (TIGR02622; EC 4.2.1.45; HMM-score: 50.1)
  Hypothetical proteinsConservedTIGR01777 family protein (TIGR01777; HMM-score: 48.1)
  Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesdTDP-4-dehydrorhamnose reductase (TIGR01214; EC 1.1.1.133; HMM-score: 47.4)
  hopanoid-associated sugar epimerase (TIGR03466; HMM-score: 45)
  thioester reductase domain (TIGR01746; HMM-score: 29)
  UDP-N-acetylglucosamine 4,6-dehydratase (inverting) (TIGR03589; EC 4.2.1.115; HMM-score: 21)
  UDP-N-acetylglucosamine 4,6-dehydratase/5-epimerase (TIGR04130; EC 4.2.1.-,5.1.3.-; HMM-score: 16.5)
  2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (TIGR04316; EC 1.3.1.28; HMM-score: 12.1)
  L-aminoadipate-semialdehyde dehydrogenase (TIGR03443; EC 1.2.1.31; HMM-score: 10.2)
• TheSEED:
• PFAM:
  NADP_Rossmann (CL0063) Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 209.6)
  GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 206.1)
  3Beta_HSD; 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073; HMM-score: 49.1)
  RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 47.6)
  Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 38.7)
  NAD_binding_4; Male sterility protein (PF07993; HMM-score: 37.8)
  NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 27.6)
  Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 19.5)
  adh_short; short chain dehydrogenase (PF00106; HMM-score: 18.6)
  KR; KR domain (PF08659; HMM-score: 15.2)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • Ymax: 0.111
  • Ymax_pos: 33
  • Cmax: 0.12
  • Cmax_pos: 27
  • Smax: 0.205
  • Smax_pos: 23
  • Smean: 0.079
  • D: 0.098
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_001793242 NCBI
• UniProt:

⊟Protein sequence

• MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENIKSLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVATLRLLEIIKKYNSHIKRFIFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTLNYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDFVYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARKGDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMS

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• SA_RS00780 no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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