PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS01640 [old locus tag: SA0281 ]
• pan locus tag^?: SAUPAN001190000
• symbol: SA_RS01640
• pan gene symbol^?: esaD
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS01640 [old locus tag: SA0281 ]
• symbol: SA_RS01640
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 338711..340555
• length: 1845
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (338711..340555) NCBI
• BioCyc: G1G21-305 BioCyc
• MicrobesOnline: see SA0281

⊟Phenotype

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⊟DNA sequence

• 1
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  1801
  
  ATGACAAAAGATATTGAATATCTAACAGCTGATTATGACAATGAAAAGTCATCTATCCAA
  AGTGTAATAGATGCAATAGAGGGTCAAGACTTCTTAGATGTAGATACAACAATGGATGAT
  GCGGTAAGCGATGTCAGTTCTTTAGACGAAGATGGCGCAATATCATTAACAAGTAGTGTA
  GTAGGTCCACAAGGATCTAAATTAATGGGGTATTATCAAAATGAGCTATATGATTATGCA
  TCTCAATTAGATTCGAAAATGAAAGAAATTATTGACACGCCATTTATAGAAGATATAGAT
  AAAGCATTCAAAGGTATAACGAATGTTAAATTGGAAAATATATTAATAAAAAATGGCGGT
  GGTCATGGTAGAGATACCTATGGGGCTTCTGGGAAAATTGCAAAGGGAGATGCCAAGAAA
  AGTGACAGCGATGTTTATAGCATCGATGAAATATTAAAATCGGATCAAGAATTTGTAAAA
  GTAATTGATCAGCATTACAAAGAAATGAAAAAAGAAGATAAGAAACTATCTAAGAGTGAT
  TTTGAAAAAATGATGACTCAAGGTGCTTCTTGTGATTACATGACAGTAGCTGAAGCGGAA
  GAGATAGAAGAGCAAAAGAAAAAAGAAGAAGCTATAGAGATTGCAGCACTAGCTGGTATG
  GTAGTTTTATCTTGTATTAATCCTGTTGCTGGAGCAGTAGCTATTGGTGCTTATTCCGCT
  TATTCAGCAGCAAATGCAGCCACAGGAAAAAATATTGTAACTGGAAGAAAGCTATCTAAA
  GAAGAACGAATCATGGAAGGACTTTCGCTTATTCCATTGCCAGGTATGGGCTTCCTCAAA
  GGTGCTGGGAAAAGTTTAATGAAATTAGGCTTCAAAGGCGGAGAAAAATTTGCAGTTAAA
  ACAGGATTGCAAAAGACAATGCAACAAGCAGTTAGTCGTATTTCACCTAAAATGGGAATG
  ATGAAAAACAGTGTGTTGAATCAATCTCGTAACTTTGCTCAAAATACTCATGTTGGACAA
  ATGCTGAGTAACATGCGTGGTCAAGCAACTCATACTGTTCAACAAAGTAGAAATTGGATT
  GGACAACAAGCACAAAATGTCAAACGAATAGTGAATAATGGACTTGATAAAGAAATAGCA
  CATCCATTTAAACAACAACTTGCACCAGCGGGAATGGGTGGTATAAAATTTGCTGAAACA
  ACTACTTTGAGAAACATGGGTCAAAACATGAAACGTGCTGTTACACCACAAAATCACGTG
  ACACATGGTCCGAAAGATAGTATGGTGAGAAGTGAAGGTAAACATAGTGTAAGTAGCCAT
  GAAATAAATTTATCAAAATATGTTGAATCACCAAACTACACCAAGGTCAAATATGGAGAG
  CAATATGCAAGACTTAGACCTAAGAAACTAAAAGCAAATATAGAATATACAACACCAACT
  GGTCACATATATCGGACTGATCATAAAGGGCGCATAAAAGAAGTTTATGTAGACAATCTT
  TCTCTAAAAGATGGCGGTCGTAATAACCATGCACAAAGAACTGTGGGAGGAGAGGATAGA
  TTACCAGACGATGATGGAGGTCACTTAATCGCCAGAATGTTTGGTGGGTCAAAAGACATA
  GATAACCTTGTAGCACAAAGTAAATATATCAACCGTTCATTTAAGGAAAATGGTGATTGG
  TACAAGTTGGAAAAACTATGGGAAAAGGCAATCAAGTCTGGAAAATCAATAGAAAATATT
  AAAATAGAAGTTAAATATAAAGGTAATAGTCAAAGGCCGACTATATTTAAAGTTGAATAT
  GAAATTAATGGTAAAAGAAATATTAGAAGAATATTAAATAAGTAG
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  1440
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  1560
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  1740
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  1845
  

⊟Protein

⊟General

• locus tag: SA_RS01640 [old locus tag: SA0281 ]
• symbol: SA_RS01640
• description: hypothetical protein
• length: 614
• theoretical pI: 9.52822
• theoretical MW: 68206
• GRAVY: -0.647557

⊟Function

• TIGRFAM:
• TheSEED: see SA0281
• PFAM:
  His-Me_finger (CL0263) Endonuclea_NS_2; DNA/RNA non-specific endonuclease (PF13930; HMM-score: 113.7)
  and 2 more
  no clan defined PT-TG; Pre-toxin TG (PF14449; HMM-score: 34.2)
  FAM180; FAM180 family (PF15173; HMM-score: 13.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00495
  • TAT(Tat/SPI): 0.000477
  • LIPO(Sec/SPII): 0.000561
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446081159 NCBI
• RefSeq: WP_000159014 NCBI
• UniProt: see SA0281

⊟Protein sequence

• MTKDIEYLTADYDNEKSSIQSVIDAIEGQDFLDVDTTMDDAVSDVSSLDEDGAISLTSSVVGPQGSKLMGYYQNELYDYASQLDSKMKEIIDTPFIEDIDKAFKGITNVKLENILIKNGGGHGRDTYGASGKIAKGDAKKSDSDVYSIDEILKSDQEFVKVIDQHYKEMKKEDKKLSKSDFEKMMTQGASCDYMTVAEAEEIEEQKKKEEAIEIAALAGMVVLSCINPVAGAVAIGAYSAYSAANAATGKNIVTGRKLSKEERIMEGLSLIPLPGMGFLKGAGKSLMKLGFKGGEKFAVKTGLQKTMQQAVSRISPKMGMMKNSVLNQSRNFAQNTHVGQMLSNMRGQATHTVQQSRNWIGQQAQNVKRIVNNGLDKEIAHPFKQQLAPAGMGGIKFAETTTLRNMGQNMKRAVTPQNHVTHGPKDSMVRSEGKHSVSSHEINLSKYVESPNYTKVKYGEQYARLRPKKLKANIEYTTPTGHIYRTDHKGRIKEVYVDNLSLKDGGRNNHAQRTVGGEDRLPDDDGGHLIARMFGGSKDIDNLVAQSKYINRSFKENGDWYKLEKLWEKAIKSGKSIENIKIEVKYKGNSQRPTIFKVEYEINGKRNIRRILNK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications