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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS03910 [old locus tag: SA0686 ]
  • pan locus tag?: SAUPAN002630000
  • symbol: SA_RS03910
  • pan gene symbol?: nrdE
  • synonym:
  • product: ribonucleotide-diphosphate reductase subunit alpha

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA_RS03910 [old locus tag: SA0686 ]
  • symbol: SA_RS03910
  • product: ribonucleotide-diphosphate reductase subunit alpha
  • replicon: chromosome
  • strand: +
  • coordinates: 783471..785576
  • length: 2106
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_002745 (783471..785576) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
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    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAAAACCATGGAAGAGAAAAAGTACAATCATATTGAATTAAATAATGAGGTCACTAAA
    CGAAGAGAAGATGGATTCTTTAGTTTAGAAAAAGACCAAGAAGCTTTAGTAGCTTATTTA
    GAAGAAGTAAAAGACAAAACAATCTTCTTCGATACTGAAATCGAGCGTTTACGTTATTTA
    GTAGACAACGATTTTTATTTCAATGTGTTTGATATTTATAGTGAAGCGGATCTAATTGAA
    ATCACTGATTATGCAAAATCAATCTCGTTTAATTTTGCAAGTTATATGTCAGCTAGTAAA
    TTTTTCAAAGATTACGCTTTGAAAACAAATGATAAAAGTCAATACTTAGAAGACTATAAT
    CAACACGTTGCCATTGTTGCTTTATACCTAGCAAATGGTAATAAAGCACAAGCTAAACAA
    TTTATTTCTGCTATGGTTGAACAAAGATATCAACCAGCGACACCAACATTTTTAAACGCA
    GGCCGTGCGCGTCGTGGTGAGCTAGTGTCATGTTTCTTATTAGAAGTGGATGACAGCTTA
    AATTCAATTAACTTTATTGATTCAACTGCAAAACAATTAAGTAAAATTGGGGGCGGCGTT
    GCAATTAACTTATCTAAATTGCGTGCACGTGGTGAAGCAATTAAAGGAATTAAAGGCGTA
    GCGAAAGGCGTTTTACCTATTGCTAAGTCACTTGAAGGTGGCTTTAGCTATGCAGATCAA
    CTTGGTCAACGCCCTGGTGCTGGTGCTGTGTACTTAAATATCTTCCATTATGATGTAGAA
    GAATTTTTAGATACTAAAAAAGTAAATGCGGATGAAGATTTACGTTTATCTACAATATCA
    ACTGGTTTAATTGTTCCATCTAAATTCTTCGATTTAGCTAAAGAAGGTAAGGACTTTTAT
    ATGTTTGCACCTCATACAGTTAAAGAAGAATATGGTGTGACATTAGACGATATCGATTTA
    GAAAAATATTATGATGACATGGTTGCAAACCCAAATGTTGAGAAAAAGAAAAAGAATGCG
    CGTGAAATGTTGAATTTAATTGCGCAAACACAATTACAATCAGGTTATCCATATTTAATG
    TTTAAAGATAATGCTAACAGAGTGCATCCGAATTCAAACATTGGACAAATTAAAATGAGT
    AACTTATGTACGGAAATTTTCCAACTACAAGAAACTTCAATTATTAATGACTATGGTATT
    GAAGACGAAATTAAACGTGATATTTCTTGTAACTTGGGCTCATTAAATATTGTTAATGTA
    ATGGAAAGCGGAAAATTCAGAGATTCAGTTCACTCTGGTATGGACGCATTAACTGTTGTG
    AGTGATGTAGCAAATATTCAAAATGCACCAGGAGTTAGAAAAGCTAACAGTGAATTACAT
    TCAGTTGGTCTTGGTGTGATGAATTTACACGGTTACCTAGCAAAAAATAAAATTGGTTAT
    GAGTCAGAAGAAGCAAAAGATTTTGCAAATATCTTCTTTATGATGATGAATTTCTACTCA
    ATCGAACGTTCAATGGAAATCGCTAAAGAGCGTGGTATCAAATATCAAGACTTTGAAAAG
    TCTGATTATGCTAATGGCAAATATTTCGAGTTCTATACAACTCAAGAATTTGAACCTCAA
    TTCGAAAAAGTACGTGAATTATTCGATGGTATGGCTATTCCTACTTCTGAGGATTGGAAG
    AAACTACAACAAGATGTTGAACAATATGGTTTATATCATGCATATAGATTAGCAATTGCT
    CCAACACAAAGTATTTCTTATGTTCAAAATGCAACAAGTTCTGTAATGCCAATCGTTGAC
    CAAATTGAACGTCGTACTTATGGTAATGCGGAAACATTTTACCCTATGCCATTCTTATCA
    CCACAAACAATGTGGTACTACAAATCAGCATTCAATACTGATCAGATGAAATTAATCGAT
    TTAATTGCGACAATTCAAACGCATATTGACCAAGGTATCTCAACGATCCTTTATGTTAAT
    TCTGAAATTTCTACACGTGAGTTAGCAAGATTATATGTATATGCGCACTATAAAGGATTA
    AAATCACTTTACTATACTAGAAATAAATTATTAAGTGTAGAAGAATGTACAAGTTGTTCT
    ATCTAA
    60
    120
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    420
    480
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    720
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    840
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    2106

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA_RS03910 [old locus tag: SA0686 ]
  • symbol: SA_RS03910
  • description: ribonucleotide-diphosphate reductase subunit alpha
  • length: 701
  • theoretical pI: 4.99873
  • theoretical MW: 80115.2
  • GRAVY: -0.372753

Function[edit source | edit]

  • reaction:
    EC 1.17.4.1?  ExPASy
    Ribonucleoside-diphosphate reductase2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin
  • TIGRFAM:
    ribonucleoside-diphosphate reductase, class 1b, alpha subunit (TIGR04170; EC 1.17.4.1; HMM-score: 1140.1)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotides2'-Deoxyribonucleotide metabolismribonucleoside-diphosphate reductase, alpha subunit (TIGR02506; EC 1.17.4.1; HMM-score: 544.3)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotides2'-Deoxyribonucleotide metabolismribonucleoside-diphosphate reductase, adenosylcobalamin-dependent (TIGR02504; EC 1.17.4.1; HMM-score: 229.4)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotides2'-Deoxyribonucleotide metabolismribonucleoside-diphosphate reductase, alpha chain (TIGR02510; EC 1.17.4.1; HMM-score: 130.9)
  • TheSEED:
  • PFAM:
    PFL-like (CL0339) Ribonuc_red_lgC; Ribonucleotide reductase, barrel domain (PF02867; HMM-score: 462.2)
    no clan definedRNR_N; Ribonucleotide reductase N-terminal (PF08343; HMM-score: 71.2)
    Ribonuc_red_lgN; Ribonucleotide reductase, all-alpha domain (PF00317; HMM-score: 51.9)
    Caud_tail_N; Caudoviral major tail protein N-terminus (PF16838; HMM-score: 14.9)
    Alk_phosphatase (CL0088) DUF4976; Domain of unknown function (DUF4976) (PF16347; HMM-score: 10.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SA_RS01275formate acetyltransferase  [1] (data from MRSA252)
    SA_RS02955elongation factor G  [1] (data from MRSA252)
    SA_RS02960elongation factor Tu  [1] (data from MRSA252)
    SA_RS04575NADH dehydrogenase  [1] (data from MRSA252)
    SA_RS0614050S ribosomal protein L19  [1] (data from MRSA252)
    SA_RS08600universal stress protein  [1] (data from MRSA252)
    SA_RS1168030S ribosomal protein S5  [1] (data from MRSA252)
    SA_RS1172030S ribosomal protein S17  [1] (data from MRSA252)
    SA_RS1175550S ribosomal protein L23  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.113
    • Ymax_pos: 11
    • Cmax: 0.104
    • Cmax_pos: 41
    • Smax: 0.146
    • Smax_pos: 1
    • Smean: 0.128
    • D: 0.119
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446778258 NCBI
  • RefSeq: WP_000855514 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVKDKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSISFNFASYMSASKFFKDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNAGRARRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIKGVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRLSTISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVEKKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQETSIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAPGVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIAKERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMAIPTSEDWKKLQQDVEQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTMWYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKSLYYTRNKLLSVEECTSCSI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SA_RS03910 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]