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NCBI: 02-MAR-2017

⊟Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS04675 [old locus tag: SA0822 ]
  • pan locus tag?: SAUPAN003068000
  • symbol: SA_RS04675
  • pan gene symbol?: argG
  • synonym:
  • product: argininosuccinate synthase

⊟Genome View[edit source | edit]

⊟Gene[edit source | edit]

⊟General[edit source | edit]

  • type: CDS
  • locus tag: SA_RS04675 [old locus tag: SA0822 ]
  • symbol: SA_RS04675
  • product: argininosuccinate synthase
  • replicon: chromosome
  • strand: -
  • coordinates: 927310..928515
  • length: 1206
  • essential: unknown

⊟Accession numbers[edit source | edit]

  • Location: NC_002745 (927310..928515) NCBI
  • MicrobesOnline:

⊟Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAAAGAGAAAATTGTTTTAGCATATTCAGGAGGACTAGATACAAGTGTGGCCGTTCAA
    TGGCTTATCGACAAAGGATACGACGTTGTAGCTTGTTGCCTAGATGTTGGTGAAGGTAAA
    GATTTAGACATCGTTTATAAAAAAGCTTTAGATATGGGAGCAGTTGAATGTCATATTATT
    GATGCAACAAAAGAATTTAGTGATGAGTATGTAAGTTATGCAATCAAAGGAAATTTAATG
    TATGAAAATGCATATCCATTAGTTTCAGCTTTATCAAGACCATTAATCGCTAAAAAATTA
    GTAGAGATTGCTGAGAAAACAAATTCAGTAGGTATTGCACATGGTTGTACTGGTAAAGGG
    AATGACCAAGTACGTTTCGAAGTTGCCATTAAAGCATTAAACCCATCATTGAAAGCATTC
    GCACCTGTACGTGAGTGGGCATGGAGTCGTGAAGAAGAAATCGATTATGCAATTAAACAT
    AATATCCCTGTATCAATCAACCATGATTCACCTTATTCTATCGATCAAAATCTATGGGGC
    AGAGCGAATGAATGTGGTATTTTAGAAGATCCTTATGCTGCGCCACCAGAGGATGCGTTC
    GATCTAACAAATGCTTTAGAAGAAACACCAGATACTGCTGATGAAATCATTTTAACGTTT
    GATAAAGGCATCCCAGTTCAAATTGATGGCAAAACATATGAATTAGACGATTTAATTTTA
    ACGTTGAATGCATTAGCTGGTAAGCATGGTATCGGAAGAATTGACCATGTAGAAAATAGA
    CTTGTAGGTATCAAATCAAGAGAAATTTATGAGGCACCTGCTGCAGAAGTTATTTTAAAA
    GCGCATAAAGCATTAGAAACGATTACGTTAACTAAAGATGTCGCACACTTTAAACCAATC
    ATTGAGAAGCAATTTGCTGAACAACTATACAATGGACTTTGGTTCTCACCTTTAACTGAT
    AGCTTGAAATTATTTATTGATAGTACTCAGCAATACGTAAGTGGTGATGTCAGAATTAAA
    TTATTCAAAGGTAATGCCATCGTGAATGGTAGAAAATCACCTTACACATTATATGATGAA
    AAATTAGCAACTTATACAAAAGAAGATGCATTTAATCAAGACGCTGCTGTTGGCTTTATC
    GATATCTATGGTTTACCTACTCAAGTAAATGCGATGTTGCATGGAGGCTATAGCAATGAG
    CAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1206

⊟Protein[edit source | edit]

⊟General[edit source | edit]

  • locus tag: SA_RS04675 [old locus tag: SA0822 ]
  • symbol: SA_RS04675
  • description: argininosuccinate synthase
  • length: 401
  • theoretical pI: 4.61504
  • theoretical MW: 44455.1
  • GRAVY: -0.182544

⊟Function[edit source | edit]

  • reaction:
    EC 6.3.4.5?  ExPASy
    Argininosuccinate synthaseATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate
  • TIGRFAM:
    MetabolismAmino acid biosynthesisGlutamate familyargininosuccinate synthase (TIGR00032; EC 6.3.4.5; HMM-score: 524.1)
    Unknown functionGeneralqueuosine biosynthesis protein QueC (TIGR00364; HMM-score: 14.3)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (TIGR00420; EC 2.1.1.61; HMM-score: 13.1)
    Hypothetical proteinsConservedTIGR00268 family protein (TIGR00268; HMM-score: 12.7)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 12.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminetRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 12.5)
  • TheSEED:
  • PFAM:
    HUP (CL0039) Arginosuc_synth; Arginosuccinate synthase (PF00764; HMM-score: 556.4)
    tRNA_Me_trans; tRNA methyl transferase (PF03054; HMM-score: 16.9)
    QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 14.2)
    ThiI; Thiamine biosynthesis protein (ThiI) (PF02568; HMM-score: 11.9)

⊟Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SA_RS02960elongation factor Tu  [1] (data from MRSA252)
    SA_RS08505aldehyde dehydrogenase  [1] (data from MRSA252)
    SA_RS1168030S ribosomal protein S5  [1] (data from MRSA252)

⊟Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.124
    • Ymax_pos: 28
    • Cmax: 0.12
    • Cmax_pos: 68
    • Smax: 0.18
    • Smax_pos: 26
    • Smean: 0.117
    • D: 0.121
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit source | edit]

  • GI: 446582699 NCBI
  • RefSeq: WP_000660045 NCBI
  • UniProt:

⊟Protein sequence[edit source | edit]

  • MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDIVYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVEIAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKHNIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIILTFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAEVILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVSGDVRIKLFKGNAIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAMLHGGYSNEQ

⊟Experimental data[edit source | edit]

  • experimentally validated: no data available

⊟Expression & Regulation[edit source | edit]

⊟Operon[edit source | edit]

  • SA_RS04675 no polycistronic organisation predicted

⊟Regulation[edit source | edit]

  • sigma factor:
  • regulator:

⊟Transcription pattern[edit source | edit]

⊟Protein synthesis (provided by Aureolib)[edit source | edit]

⊟Protein stability[edit source | edit]

  • half-life: no data available

⊟Biological Material[edit source | edit]

⊟Mutants[edit source | edit]

⊟Expression vector[edit source | edit]

⊟lacZ fusion[edit source | edit]

⊟GFP fusion[edit source | edit]

⊟two-hybrid system[edit source | edit]

⊟FLAG-tag construct[edit source | edit]

⊟Antibody[edit source | edit]

⊟Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

⊟Literature[edit source | edit]

⊟References[edit source | edit]

  1. ↑ 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit source | edit]

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