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NCBI: 02-MAR-2017

⊟Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS04680 [old locus tag: SA0823 ]
  • pan locus tag?: SAUPAN003070000
  • symbol: SA_RS04680
  • pan gene symbol?: pgi
  • synonym:
  • product: glucose-6-phosphate isomerase

⊟Genome View[edit source | edit]

⊟Gene[edit source | edit]

⊟General[edit source | edit]

  • type: CDS
  • locus tag: SA_RS04680 [old locus tag: SA0823 ]
  • symbol: SA_RS04680
  • product: glucose-6-phosphate isomerase
  • replicon: chromosome
  • strand: +
  • coordinates: 928866..930197
  • length: 1332
  • essential: unknown

⊟Accession numbers[edit source | edit]

  • Location: NC_002745 (928866..930197) NCBI
  • MicrobesOnline:

⊟Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGACTCATATTCAATTAGATTTTAGTAAAACGTTAGAATTTTTCGGTGAACACGAATTA
    AAACAACAACAAGAAATTGTTAAATCAATTCACAAAACAATTCATGAAGGTACTGGTGCA
    GGTAGTGACTTCTTAGGCTGGGTTGATTTACCAGTTGATTACGACAAAGAAGAATTTTCA
    AGAATTGTTGAAGCATCAAAACGCATTAAAGAAAATTCTGATGTTTTAGTAGTCATCGGT
    ATTGGTGGTTCTTACTTAGGTGCACGTGCAGCAATCGAAATGTTAACGTCATCATTTAGA
    AACAGCAATGAATACCCTGAAATTGTATTTGTTGGTAATCACTTATCATCAACATATACG
    AAAGAGTTAGTTGATTATTTAGCAGACAAAGATTTCTCTGTAAACGTTATTTCTAAATCT
    GGTACAACTACAGAACCAGCAGTTGCATTTAGATTGTTCAAACAATTAGTTGAAGAAAGA
    TACGGTAAAGAAGAAGCACAAAAACGTATATTTGCAACAACGGATAAAGAAAAAGGTGCT
    TTAAAACAGTTGGCTACAAACGAAGGTTATGAAACGTTTATCGTACCTGATGATGTAGGT
    GGAAGATATTCTGTTTTAACAGCAGTAGGATTATTACCAATTGCAACAGCTGGAATTAAC
    ATCGAAGCTATGATGATTGGTGCTGCAAAAGCACGTGAAGAATTATCTTCAGATAAATTA
    GAAGACAACATTGCATACCAATATGCGACAATTCGAAACATTTTATATGCAAAAGGTTAT
    ACAACAGAAATGTTGATTAACTATGAACCATCTATGCAATACTTTAATGAATGGTGGAAA
    CAATTATTTGGTGAATCAGAAGGTAAAGACTTCAAAGGTATCTATCCTTCAAGTGCCAAC
    TACACAACTGATTTACATTCTTTAGGTCAATATGTACAAGAAGGCCGTCGTTTCTTATTC
    GAAACAGTGGTAAAAGTAAATCATCCTAAATATGATATTACTATTGAAAAAGATAGTGAT
    GATCTAGACGGATTAAATTATTTGGCTGGTAAAACAATCGACGAAGTTAACACAAAAGCA
    TTCGAAGGTACATTATTAGCGCATACTGATGGTGGTGTTCCTAACATGGTAGTGAACATT
    CCACAATTAGATGAAGAAACTTTCGGTTATGTCGTATACTTCTTCGAACTTGCTTGTGCA
    ATGAGTGGATACCAATTAGGTGTAAATCCATTTAACCAACCTGGTGTAGAAGCATATAAA
    CAAAACATGTTCGCATTATTAGGTAAACCTGGTTTTGAAGACTTGAAAAAAGAATTAGAA
    GAGCGTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1332

⊟Protein[edit source | edit]

⊟General[edit source | edit]

  • locus tag: SA_RS04680 [old locus tag: SA0823 ]
  • symbol: SA_RS04680
  • description: glucose-6-phosphate isomerase
  • length: 443
  • theoretical pI: 4.54452
  • theoretical MW: 49793.7
  • GRAVY: -0.325959

⊟Function[edit source | edit]

  • reaction:
    EC 5.3.1.9?  ExPASy
    Glucose-6-phosphate isomeraseD-glucose 6-phosphate = D-fructose 6-phosphate
  • TIGRFAM:
    bifunctional phosphoglucose/phosphomannose isomerase (TIGR02128; EC 5.3.1.9; HMM-score: 24.8)
    and 1 more
    dihydroxyacetone kinase, phosphotransfer subunit (TIGR02364; HMM-score: 13.8)
  • TheSEED:
  • PFAM:
    SIS (CL0067) PGI; Phosphoglucose isomerase (PF00342; HMM-score: 82.2)
    and 3 more
    no clan definedELMO_CED12; ELMO/CED-12 family (PF04727; HMM-score: 14.8)
    GT-B (CL0113) Glyco_trans_4_2; Glycosyl transferase 4-like (PF13477; HMM-score: 14.6)
    EDD (CL0245) EIIA-man; PTS system fructose IIA component (PF03610; HMM-score: 13.2)

⊟Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SA_RS0614050S ribosomal protein L19  [1] (data from MRSA252)
    SA_RS0846050S ribosomal protein L20  [1] (data from MRSA252)
    SA_RS08560pyruvate kinase  [1] (data from MRSA252)
    SA_RS1175050S ribosomal protein L2  [1] (data from MRSA252)
    SA_RS1175550S ribosomal protein L23  [1] (data from MRSA252)

⊟Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.116
    • Ymax_pos: 30
    • Cmax: 0.112
    • Cmax_pos: 30
    • Smax: 0.138
    • Smax_pos: 21
    • Smean: 0.096
    • D: 0.108
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit source | edit]

  • GI: 446070997 NCBI
  • RefSeq: WP_000148852 NCBI
  • UniProt:

⊟Protein sequence[edit source | edit]

  • MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDFLGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNSNEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEERYGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAGINIEAMMIGAAKAREELSSDKLEDNIAYQYATIRNILYAKGYTTEMLINYEPSMQYFNEWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDITIEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVVYFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL

⊟Experimental data[edit source | edit]

  • experimentally validated: no data available

⊟Expression & Regulation[edit source | edit]

⊟Operon[edit source | edit]

  • SA_RS04680 no polycistronic organisation predicted

⊟Regulation[edit source | edit]

  • sigma factor:
  • regulator:

⊟Transcription pattern[edit source | edit]

⊟Protein synthesis (provided by Aureolib)[edit source | edit]

⊟Protein stability[edit source | edit]

  • half-life: no data available

⊟Biological Material[edit source | edit]

⊟Mutants[edit source | edit]

⊟Expression vector[edit source | edit]

⊟lacZ fusion[edit source | edit]

⊟GFP fusion[edit source | edit]

⊟two-hybrid system[edit source | edit]

⊟FLAG-tag construct[edit source | edit]

⊟Antibody[edit source | edit]

⊟Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

⊟Literature[edit source | edit]

⊟References[edit source | edit]

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit source | edit]

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