PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 22-FEB-2026

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: EGJ38_RS00875 [old locus tag: EGJ38_000174 ]
• pan locus tag^?: SAUPAN001102000
• symbol: nikA
• pan gene symbol^?: nikA
• synonym: opp5A
• product: nickel ABC transporter substrate-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: EGJ38_RS00875 [old locus tag: EGJ38_000174 ]
• symbol: nikA
• product: nickel ABC transporter substrate-binding protein
• replicon: chromosome
• strand: -
• coordinates: 213994..215469
• length: 1476
• essential: unknown other strains

⊟Accession numbers

• Location: NZ_CM129921 (213994..215469) NCBI
• BioCyc:
• MicrobesOnline:

⊟Phenotype

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⊟DNA sequence

• 1
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  481
  541
  601
  661
  721
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  901
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  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  
  ATGAAGTTTAAAAGACTAGCAACTATATTTTCAGCAGTATTAGTGTTATCAGGATGTGGC
  TCAATGCATTCATCTGGCAAAGACTTAAATATTTCGTTACCGTTAAAAACAAAGTCTATT
  GCACCTTATGAAACAGATGTACCAGTTAAGATTGGTGCTGCAGAATCATTATTTAAAACA
  AATGATCAAGGAAAAATAGAAAAAGCATTAGTGAAATCGTATCATCAACCAAATGATACA
  ACGTTAGATATAGAATTAAAAGATAATATTAAATTTCAAAACGGTCAAAAATTGACTGCA
  GAAAAAGTGAAATCTAGCCTTGAAAATAGCATGAAAAAAAGCGACTTGGTCAAATATTCA
  TTACCAATATCATCAATTACCGCTAAAGGTCAAAAACTGACAATTAAAACCAACTCCGCT
  TACCCTGAACTTGTATCTGAATTAGCTAATCCTTTTATGGCAATTTATGATACAGATGCT
  AAATCAGATGTTAATCAAACTCCTGTTGGTACAGGCCCTTACCAAATAAAAGATTATAAG
  CAATCTCGAAAAATATCATTGTCGAATTTTAAGGACTATTGGCAAGGTAAACCCAAACTT
  GATCATATTACTGTGACGTACCAAGAAGACGGCAATAATCGCGTCAGAAATTTAGAATCT
  CAAAAAGATGATTTAATAACTGATGTCCCAGTTAATAAAGTTCAAGACATAGAAAATAAT
  CAAAATTTAAAAGTGTCAAAAGAATCTGGATTTAGAACTTCTTTACTTATGTATAATCAT
  ACTAATAAAAAAATGACTAAATCCGTTCGTGAAGCATTAGATCATATCATTGATAGACAA
  GGTATTGCAGATCATATTTATCAAGGTTATGCGAAACCTGCAACGAGTCCATTTAATGAT
  AAAATTCCATATATTAAAGAACCTAAGTTAACCAAACAAAATATCGAACAAGCAAAAACG
  TTATTAGCTAAAGATGGTTATACAAAAGAACACCCTTTAAAAATTAAGTTAATTACGTAT
  GACGGTCGTCCAGAGCTATCGAAAATTGCGCAAGTATTACAATCAGATGCTAAAAAAGCA
  AATATTGAAATCGACATTAAAAGTGTTGATGACATAGAAGGTTACTTAAAAGACCGTTCT
  GCATGGGATGCAACGATGTATAGTTTTGGGACAATCCCTCGCGGTGATACAGGTTATTTC
  TTTAATCAAGCATATAAAAAAGATGGTGCTATTAATAAAGGAGACTACAATAATAGCAAC
  GTCGATGATTTAATTAATCAATTGAATCATACTGTAGATGTTAAGGAACGTCACAATATT
  TCTAATGATATTATTAAATTATCAAGTCGAGATGTACCAAACAGTTATATTGCTTACAAC
  GATCAAATAGTCGCTGCTAACTCTAAGGTTAAAAATTATAAAGTAACACCTGAAGGTATC
  TATTTAATCGATTATCGAACAACGATTGAACGATAA
  60
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  1476
  

⊟Protein

⊟General

• locus tag: EGJ38_RS00875 [old locus tag: EGJ38_000174 ]
• symbol: NikA
• description: nickel ABC transporter substrate-binding protein
• length: 491
• theoretical pI: 9.5255
• theoretical MW: 55400.4
• GRAVY: -0.675356

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 117.9)
  and 2 more
  Metabolism Transport and binding proteins Unknown substrate ABC transporter substrate binding protein, KPN_01854 family (TIGR04028; HMM-score: 42.4)
  glycosyltransferase, SP_1767 family (TIGR03728; HMM-score: 17.2)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 226)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0.01
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 9.2
  • Extracellular Score: 0.78
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0001
  • Cytoplasmic Membrane Score: 0.6865
  • Cell wall & surface Score: 0.0065
  • Extracellular Score: 0.3069
• LocateP:
• SignalP: Signal peptide LIPO(Sec/SPII) length 18 aa
  • SP(Sec/SPI): 0.002815
  • TAT(Tat/SPI): 0.000092
  • LIPO(Sec/SPII): 0.996788
  • Cleavage Site: CS pos: 18-19. LSG-CG. Pr: 0.9975
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_000669655 NCBI
• UniProt:

⊟Protein sequence

• MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYETDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEKVKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDAKSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNLESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHIIDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKTLLAKDGYTKEHPLKIKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTIPRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSRDVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications