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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000204
  • pan locus tag?: SAUPAN001143000
  • symbol: JSNZ_000204
  • pan gene symbol?: bglR
  • synonym:
  • product: GntR family transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000204
  • symbol: JSNZ_000204
  • product: GntR family transcriptional regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 245124..245828
  • length: 705
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    TTGTTAAAGTATGAACATATTGCTAAGCAACTTAATGCGTTTATACATCAATCTAATTTC
    AAACCCGGTGATAAATTGCCAAGCGTGACGCAATTAAAAGAACGTTATCAAGTAAGTAAG
    AGTACTATCATTAAAGCATTAGGCTTATTGGAACAAGATGGTTTGATCTATCAAGCACAA
    GGCAGTGGTATTTATGTGAGAAATATTGCAGATGCCAATCGTATCAACGTCTTTAAGACT
    AATGGTTTCTCTAAAAGTTTAGGTGAACATCGAATGACAAGTAAGGTACTTGTTTTTAAG
    GAGATGGCAACGCCACCTAAATCTGTACAAGATGAGCTCCAATTAAATGCAGATGATACC
    GTCTACTATTTAGAGCGATTAAGATTCGTGGACGATGATGTTTTATGTATCGAATATTCT
    TATTATCATAAAGAAATCGTGAAATATTTAAATGATGATATTGCTAAGGGCTCTATCTTC
    GATTATTTAGAATCAAACATGAAACTTCGTATTGGTTTTTCAGATATTTTCTTTAATGTA
    GATAAACTCACTTCAAGTGAAGCTTCATTACTACAATTGTCTACAGGTGAACCATGTTTA
    CGTTACCACCAGACTTTTTATACAATGACTGGCAAACCCTTTGATTCATCTGACATCGTA
    TTTCATTATCGTCATGCACAGTTTTATATTCCTAGTAAAAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    705

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000204
  • symbol: JSNZ_000204
  • description: GntR family transcriptional regulator
  • length: 234
  • theoretical pI: 8.17816
  • theoretical MW: 27031.7
  • GRAVY: -0.37265

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02404; HMM-score: 112.9)
    Signal transduction Regulatory functions DNA interactions phosphonate utilization transcriptional regulator PhnR (TIGR03337; HMM-score: 90.4)
    and 10 more
    Metabolism Transport and binding proteins Anions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 77.7)
    Signal transduction Regulatory functions DNA interactions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 77.7)
    Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 67)
    Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 67)
    phosphonate utilization associated transcriptional regulator (TIGR03338; HMM-score: 27.8)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 17.5)
    Metabolism Fatty acid and phospholipid metabolism Degradation fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 17.5)
    Signal transduction Regulatory functions DNA interactions fatty acid metabolism transcriptional regulator FadR (TIGR02812; HMM-score: 17.5)
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 13)
    Unknown function General Rrf2 family protein (TIGR00738; HMM-score: 12.1)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    UTRA (CL0122) UTRA; UTRA domain (PF07702; HMM-score: 94.6)
    and 12 more
    HTH (CL0123) GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 61.1)
    HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 22.2)
    HTH_41; Helix-turn-helix domain (PF14502; HMM-score: 21.9)
    HTH_11; HTH domain (PF08279; HMM-score: 17)
    MarR_2; MarR family (PF12802; HMM-score: 16.2)
    Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 15.5)
    TBPIP; TBPIP/Hop2 winged helix domain (PF07106; HMM-score: 15.3)
    DnaD_N; DnaD N-terminal domain (PF21984; HMM-score: 14.9)
    Rrf2; Iron-dependent Transcriptional regulator (PF02082; HMM-score: 14.7)
    MarR; MarR family (PF01047; HMM-score: 14.1)
    HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 12.9)
    RPA_C; Replication protein A C terminal (PF08784; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effector: Beta-glucoside-6-phosphate
  • genes regulated by BglR*, TF important in Beta-glucosides utilizationsee RegPrecise for N315
    repression
    bglR*
    JSNZ_000205 > bglA*

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9839
    • Cytoplasmic Membrane Score: 0.0076
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0084
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00433
    • TAT(Tat/SPI): 0.000361
    • LIPO(Sec/SPII): 0.000474
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MLKYEHIAKQLNAFIHQSNFKPGDKLPSVTQLKERYQVSKSTIIKALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEMATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIFDYLESNMKLRIGFSDIFFNVDKLTSSEASLLQLSTGEPCLRYHQTFYTMTGKPFDSSDIVFHYRHAQFYIPSKK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: BglR* (repression) regulon
    BglR*(TF)important in Beta-glucosides utilization;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Semen A Leyn, Marat D Kazanov, Natalia V Sernova, Ekaterina O Ermakova, Pavel S Novichkov, Dmitry A Rodionov
Genomic reconstruction of the transcriptional regulatory network in Bacillus subtilis.
J Bacteriol: 2013, 195(11);2463-73
[PubMed:23504016] [WorldCat.org] [DOI] (I p)

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