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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

organism: Staphylococcus aureus JSNZ
locus tag: JSNZ_001715
pan locus tag^?: SAUPAN004388000
symbol: tyrS
pan gene symbol^?: tyrS
synonym:
product: tyrosine--tRNA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: JSNZ_001715
symbol: tyrS
product: tyrosine--tRNA ligase
replicon: chromosome
strand: -
coordinates: 1757261..1758523
length: 1263
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Gene ID:
RefSeq:
BioCyc:
MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGACGAATGTATTAATTGAAGATTTAAAATGGAGAGGTCTTATTTATCAACAAACTGAT
GAACAAGGTATTGAAGATTTATTAAATAAAGAACAAGTGACGTTATACTGCGGTGCCGAT
CCAACGGCAGATAGTTTACATATTGGTCACTTACTACCGTTCTTAACATTAAGACGTTTT
CAAGAACATGGACATCGTCCTATCGTTTTAATTGGCGGTGGTACAGGTATGATTGGTGAT
CCATCAGGTAAATCAGAAGAACGTGTGCTACAAACAGAAGAACAAGTAGATAAAAATATC
GAAGGTATTAGTAAGCAAATGCACAATATTTTTGAATTTGGAACAGACCATGGTGCAGTG
CTTGTTAATAATAGAGATTGGTTAGGACAAATTTCATTAATTAGCTTTTTACGTGACTAT
GGTAAACACGTCGGCGTTAATTACATGTTAGGTAAAGATTCAATCCAAAGTCGTTTAGAA
CATGGTATTTCATATACAGAATTCACATACACGATTTTACAAGCTATTGATTTCGGTCAT
TTGAATAGAGAATTGAATTGTAAGATTCAAGTAGGTGGATCAGATCAATGGGGTAATATC
ACAAGTGGTATTGAATTAATGCGTCGTATGTATGGTCAAACAGACGCATACGGTTTAACT
ATTCCGCTTGTAACTAAATCAGATGGTAAGAAATTTGGTAAGTCTGAGTCAGGTGCTGTT
TGGTTAGATGCTGAAAAAACAAGTCCTTATGAATTTTATCAATTCTGGATTAATCAATCA
GACGAAGATGTAATTAAATTCTTAAAATACTTTACTTTCTTAGGAAAAGAAGAAATTGAT
CGCTTAGAGCAATCTAAAAATGAAGCACCGCATTTACGTGAAGCTCAAAAAACATTAGCT
GAAGAAGTAACTAAATTTATTCATGGTGAAGATGCATTAAATGATGCAATCCGTATTTCA
CAAGCATTATTTAGTGGTGATTTAAAATCATTATCAGCGAAAGAATTAAAAGATGGATTT
AAAGATGTGCCTCAAGTGACATTATCAAATGACACAACAAATATCGTTGAAGTCCTTATT
GAAACAGGCATTTCTCCTTCTAAACGACAAGCACGTGAAGATGTTAACAATGGTGCGATT
TATATTAATGGTGAGAGACAACAAGATGTTAATTATGCTTTAGCACCAGAAGATAAAATT
GATGGCGAATTTACGATTATTCGTCGCGGTAAGAAAAAATACTTCATGGTTAACTATCAA
TAA
60
120
180
240
300
360
420
480
540
600
660
720
780
840
900
960
1020
1080
1140
1200
1260
1263

⊟Protein[edit | edit source]

Protein Data Bank: 1JII

Protein Data Bank: 1JIJ

Protein Data Bank: 1JIK

Protein Data Bank: 1JIL

⊟General[edit | edit source]

locus tag: JSNZ_001715
symbol: TyrS
description: tyrosine--tRNA ligase
length: 420
theoretical pI: 5.05025
theoretical MW: 47597.2
GRAVY: -0.488333

⊟Function[edit | edit source]

reaction:
EC 6.1.1.1? ExPASy
Tyrosine--tRNA ligase ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
TIGRFAM:
Genetic information processing Protein synthesis tRNA aminoacylation tyrosine--tRNA ligase (TIGR00234; EC 6.1.1.1; HMM-score: 446.5)
and 2 more
Genetic information processing Protein synthesis tRNA aminoacylation tryptophan--tRNA ligase (TIGR00233; EC 6.1.1.2; HMM-score: 13.7)
Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein uS4 (TIGR01017; HMM-score: 12.7)
TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
PFAM:
HUP (CL0039) tRNA-synt_1b; tRNA synthetases class I (W and Y) (PF00579; HMM-score: 292.1)
and 3 more
S4 (CL0492) SYY_C-terminal; Tyrosine--tRNA ligase SYY-like C-terminal domain (PF22421; HMM-score: 126.5)
S4; S4 domain (PF01479; HMM-score: 34.5)
Metallo-HOrase (CL0381) ODP; ODP family beta lactamase (PF19583; HMM-score: 13.2)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9978
- Cytoplasmic Membrane Score: 0.0001
- Cell wall & surface Score: 0
- Extracellular Score: 0.0021
LocateP:
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002263
- TAT(Tat/SPI): 0.000233
- LIPO(Sec/SPII): 0.000469
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI:
RefSeq:
UniProt:

⊟Protein sequence[edit | edit source]

MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTADSLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEGISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLEHGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYGLTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGKEEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAKELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNYALAPEDKIDGEFTIIRRGKKKYFMVNYQ

⊟Experimental data[edit | edit source]

experimentally validated: data available for COL, NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell: data available for COL
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

data available for COL, N315, Newman, USA300_FPR3757

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: data available for COL

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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