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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1675 [new locus tag: SAUSA300_RS09145 ]
  • pan locus tag?: SAUPAN004388000
  • symbol: tyrS
  • pan gene symbol?: tyrS
  • synonym:
  • product: tyrosyl-tRNA synthetase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1675 [new locus tag: SAUSA300_RS09145 ]
  • symbol: tyrS
  • product: tyrosyl-tRNA synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1841464..1842726
  • length: 1263
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACGAATGTATTAATTGAAGATTTAAAATGGAGAGGTCTTATTTATCAACAAACTGAT
    GAACAAGGTATTGAAGATTTATTAAATAAAGAACAAGTGACGTTATACTGCGGTGCCGAT
    CCAACGGCAGATAGTTTACATATTGGTCACTTACTACCATTCTTAACATTAAGACGTTTT
    CAAGAACATGGACATCGTCCTATCGTTTTAATTGGCGGTGGTACAGGTATGATTGGTGAT
    CCATCAGGTAAATCAGAAGAACGTGTGCTACAAACAGAAGAACAAGTAGATAAAAATATC
    GAAGGTATTAGTAAGCAAATGCACAATATTTTTGAATTTGGAACAGACCATGGTGCAGTG
    CTTGTTAATAATAGAGACTGGTTAGGACAAATCTCATTAATTAGTTTTTTACGTGACTAT
    GGTAAACACGTCGGCGTTAATTACATGTTAGGTAAAGATTCAATCCAAAGTCGTTTAGAA
    CATGGTATTTCATATACAGAATTCACATACACGATTTTACAAGCTATTGATTTCGGTCAT
    TTGAATAGAGAATTGAATTGTAAGATTCAAGTAGGTGGATCAGATCAATGGGGTAATATC
    ACAAGTGGTATTGAATTAATGCGTCGTATGTATGGTCAAACAGACGCATACGGTTTAACT
    ATTCCGCTTGTAACTAAATCAGATGGTAAGAAATTTGGTAAGTCTGAGTCAGGTGCTGTT
    TGGTTAGATGCTGAAAAAACAAGTCCTTATGAATTTTATCAATTCTGGATTAATCAATCA
    GACGAAGATGTAATTAAATTCTTAAAATACTTTACTTTCTTAGGAAAAGAAGAAATTGAT
    CGCTTAGAACAATCTAAAAATGAAGCACCGCATTTACGTGAAGCTCAAAAAACATTAGCT
    GAAGAAGTAACTAAATTTATTCATGGTGAAGATGCATTAAATGATGCAATCCGTATTTCA
    CAAGCATTATTTAGTGGTGATTTAAAATCATTATCAGCGAAAGAATTAAAAGATGGATTT
    AAAGATGTGCCTCAAGTGACATTATCAAATGACACAACAAATATCGTTGAAGTCCTTATT
    GAAACAGGCATTTCTCCTTCTAAACGACAAGCACGTGAAGATGTTAACAATGGTGCGATT
    TATATTAATGGTGAGAGACAACAAGATGTTAATTATGCTTTAGCACCAGAAGATAAAATT
    GATGGCGAATTTACGATTATTCGTCGCGGTAAGAAAAAATACTTCATGGTTAACTATCAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1263

Protein[edit | edit source]

Protein Data Bank: 1JII
Protein Data Bank: 1JIJ
Protein Data Bank: 1JIK
Protein Data Bank: 1JIL

General[edit | edit source]

  • locus tag: SAUSA300_1675 [new locus tag: SAUSA300_RS09145 ]
  • symbol: TyrS
  • description: tyrosyl-tRNA synthetase
  • length: 420
  • theoretical pI: 5.05025
  • theoretical MW: 47597.2
  • GRAVY: -0.488333

Function[edit | edit source]

  • reaction:
    EC 6.1.1.1?  ExPASy
    Tyrosine--tRNA ligase ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation tyrosine--tRNA ligase (TIGR00234; EC 6.1.1.1; HMM-score: 446.5)
    and 2 more
    Genetic information processing Protein synthesis tRNA aminoacylation tryptophan--tRNA ligase (TIGR00233; EC 6.1.1.2; HMM-score: 13.7)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein uS4 (TIGR01017; HMM-score: 12.7)
  • TheSEED  :
    • Tyrosyl-tRNA synthetase (EC 6.1.1.1)
    tRNA synthetase relationships  Tyrosyl-tRNA synthetase (EC 6.1.1.1)
  • PFAM:
    HUP (CL0039) tRNA-synt_1b; tRNA synthetases class I (W and Y) (PF00579; HMM-score: 284.1)
    and 1 more
    S4 (CL0492) S4; S4 domain (PF01479; HMM-score: 34.2)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002263
    • TAT(Tat/SPI): 0.000233
    • LIPO(Sec/SPII): 0.000469
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTADSLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEGISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLEHGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYGLTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGKEEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAKELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNYALAPEDKIDGEFTIIRRGKKKYFMVNYQ

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAUSA300_1124(fabG)3-oxoacyl-(acyl-carrier-protein) reductase  [1] (data from MRSA252)
    SAUSA300_1640(icd)isocitrate dehydrogenase  [1] (data from MRSA252)
    SAUSA300_0996(lpdA)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    SAUSA300_0993(pdhA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    SAUSA300_0994(pdhB)pyruvate dehydrogenase E1 component, beta subunit  [1] (data from MRSA252)
    SAUSA300_1644(pyk)pyruvate kinase  [1] (data from MRSA252)
    SAUSA300_0523(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    SAUSA300_2192(rplE)50S ribosomal protein L5  [1] (data from MRSA252)
    SAUSA300_2185(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    SAUSA300_1134(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    SAUSA300_1603(rplU)50S ribosomal protein L21  [1] (data from MRSA252)
    SAUSA300_2198(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    SAUSA300_1306(sucA)2-oxoglutarate dehydrogenase E1 component  [1] (data from MRSA252)
    SAUSA300_0533(tuf)elongation factor Tu  [1] (data from MRSA252)
    SAUSA300_0844hypothetical protein  [1] (data from MRSA252)
    SAUSA300_0995branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: T-box(Tyr) (transcription antitermination) regulon
    T-box(Tyr)(RNA)important in Amino acid metabolism; regulatory site identified based on RegPrecise data for N315 RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]