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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_1675 [new locus tag: SAUSA300_RS09145 ]
- pan locus tag?: SAUPAN004388000
- symbol: tyrS
- pan gene symbol?: tyrS
- synonym:
- product: tyrosyl-tRNA synthetase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_1675 [new locus tag: SAUSA300_RS09145 ]
- symbol: tyrS
- product: tyrosyl-tRNA synthetase
- replicon: chromosome
- strand: -
- coordinates: 1841464..1842726
- length: 1263
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3914292 NCBI
- RefSeq: YP_494369 NCBI
- BioCyc: see SAUSA300_RS09145
- MicrobesOnline: 1293190 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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1261ATGACGAATGTATTAATTGAAGATTTAAAATGGAGAGGTCTTATTTATCAACAAACTGAT
GAACAAGGTATTGAAGATTTATTAAATAAAGAACAAGTGACGTTATACTGCGGTGCCGAT
CCAACGGCAGATAGTTTACATATTGGTCACTTACTACCATTCTTAACATTAAGACGTTTT
CAAGAACATGGACATCGTCCTATCGTTTTAATTGGCGGTGGTACAGGTATGATTGGTGAT
CCATCAGGTAAATCAGAAGAACGTGTGCTACAAACAGAAGAACAAGTAGATAAAAATATC
GAAGGTATTAGTAAGCAAATGCACAATATTTTTGAATTTGGAACAGACCATGGTGCAGTG
CTTGTTAATAATAGAGACTGGTTAGGACAAATCTCATTAATTAGTTTTTTACGTGACTAT
GGTAAACACGTCGGCGTTAATTACATGTTAGGTAAAGATTCAATCCAAAGTCGTTTAGAA
CATGGTATTTCATATACAGAATTCACATACACGATTTTACAAGCTATTGATTTCGGTCAT
TTGAATAGAGAATTGAATTGTAAGATTCAAGTAGGTGGATCAGATCAATGGGGTAATATC
ACAAGTGGTATTGAATTAATGCGTCGTATGTATGGTCAAACAGACGCATACGGTTTAACT
ATTCCGCTTGTAACTAAATCAGATGGTAAGAAATTTGGTAAGTCTGAGTCAGGTGCTGTT
TGGTTAGATGCTGAAAAAACAAGTCCTTATGAATTTTATCAATTCTGGATTAATCAATCA
GACGAAGATGTAATTAAATTCTTAAAATACTTTACTTTCTTAGGAAAAGAAGAAATTGAT
CGCTTAGAACAATCTAAAAATGAAGCACCGCATTTACGTGAAGCTCAAAAAACATTAGCT
GAAGAAGTAACTAAATTTATTCATGGTGAAGATGCATTAAATGATGCAATCCGTATTTCA
CAAGCATTATTTAGTGGTGATTTAAAATCATTATCAGCGAAAGAATTAAAAGATGGATTT
AAAGATGTGCCTCAAGTGACATTATCAAATGACACAACAAATATCGTTGAAGTCCTTATT
GAAACAGGCATTTCTCCTTCTAAACGACAAGCACGTGAAGATGTTAACAATGGTGCGATT
TATATTAATGGTGAGAGACAACAAGATGTTAATTATGCTTTAGCACCAGAAGATAAAATT
GATGGCGAATTTACGATTATTCGTCGCGGTAAGAAAAAATACTTCATGGTTAACTATCAA
TAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_1675 [new locus tag: SAUSA300_RS09145 ]
- symbol: TyrS
- description: tyrosyl-tRNA synthetase
- length: 420
- theoretical pI: 5.05025
- theoretical MW: 47597.2
- GRAVY: -0.488333
⊟Function[edit | edit source]
- reaction: EC 6.1.1.1? ExPASyTyrosine--tRNA ligase ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
- TIGRFAM: Protein synthesis tRNA aminoacylation tyrosine--tRNA ligase (TIGR00234; EC 6.1.1.1; HMM-score: 446.5)and 2 moreProtein synthesis tRNA aminoacylation tryptophan--tRNA ligase (TIGR00233; EC 6.1.1.2; HMM-score: 13.7)Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein uS4 (TIGR01017; HMM-score: 12.7)
- TheSEED :
- Tyrosyl-tRNA synthetase (EC 6.1.1.1)
- PFAM: HUP (CL0039) tRNA-synt_1b; tRNA synthetases class I (W and Y) (PF00579; HMM-score: 284.1)and 1 moreS4 (CL0492) S4; S4 domain (PF01479; HMM-score: 34.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002263
- TAT(Tat/SPI): 0.000233
- LIPO(Sec/SPII): 0.000469
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTADSLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEGISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLEHGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYGLTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGKEEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAKELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNYALAPEDKIDGEFTIIRRGKKKYFMVNYQ
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SAUSA300_1124 (fabG) 3-oxoacyl-(acyl-carrier-protein) reductase [1] (data from MRSA252) SAUSA300_1640 (icd) isocitrate dehydrogenase [1] (data from MRSA252) SAUSA300_0996 (lpdA) dihydrolipoamide dehydrogenase [1] (data from MRSA252) SAUSA300_0993 (pdhA) pyruvate dehydrogenase E1 component, alpha subunit [1] (data from MRSA252) SAUSA300_0994 (pdhB) pyruvate dehydrogenase E1 component, beta subunit [1] (data from MRSA252) SAUSA300_1644 (pyk) pyruvate kinase [1] (data from MRSA252) SAUSA300_0523 (rplA) 50S ribosomal protein L1 [1] (data from MRSA252) SAUSA300_2192 (rplE) 50S ribosomal protein L5 [1] (data from MRSA252) SAUSA300_2185 (rplO) 50S ribosomal protein L15 [1] (data from MRSA252) SAUSA300_1134 (rplS) 50S ribosomal protein L19 [1] (data from MRSA252) SAUSA300_1603 (rplU) 50S ribosomal protein L21 [1] (data from MRSA252) SAUSA300_2198 (rpsC) 30S ribosomal protein S3 [1] (data from MRSA252) SAUSA300_1306 (sucA) 2-oxoglutarate dehydrogenase E1 component [1] (data from MRSA252) SAUSA300_0533 (tuf) elongation factor Tu [1] (data from MRSA252) SAUSA300_0844 hypothetical protein [1] (data from MRSA252) SAUSA300_0995 branched-chain alpha-keto acid dehydrogenase subunit E2 [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator: T-box(Tyr) (transcription antitermination) regulon
T-box(Tyr) (RNA) important in Amino acid metabolism; regulatory site identified based on RegPrecise data for N315 RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)