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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1550 [new locus tag: SA_RS08735 ]
  • pan locus tag?: SAUPAN004388000
  • symbol: tyrS
  • pan gene symbol?: tyrS
  • synonym:
  • product: tyrosyl-tRNA synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1550 [new locus tag: SA_RS08735 ]
  • symbol: tyrS
  • product: tyrosyl-tRNA synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1772528..1773790
  • length: 1263
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACGAATGTATTAATTGAAGATTTAAAATGGAGAGGTCTTATTTATCAACAAACTGAT
    GAACAAGGTATTGAAGATTTATTAAATAAAGAACAAGTGACGTTATACTGCGGTGCCGAT
    CCAACGGCAGATAGTTTACATATTGGTCACTTACTACCATTCTTAACATTAAGACGTTTT
    CAAGAACATGGACATCGTCCTATCGTTTTAATTGGCGGTGGTACAGGTATGATTGGTGAT
    CCATCAGGTAAATCAGAAGAACGTGTGCTACAAACAGAAGAACAAGTAGATAAAAATATC
    GAAGGTATTAGTAAGCAAATGCACAATATTTTTGAATTTGGAACAGACCATGGTGCAGTG
    CTTGTTAATAATAGAGACTGGTTAGGACAAATCTCATTAATTAGCTTTTTACGTGACTAT
    GGTAAACACGTCGGCGTTAATTACATGTTAGGTAAAGATTCAATCCAAAGTCGTTTAGAA
    CATGGTATTTCATATACAGAATTCACATACACGATTTTACAAGCTATTGATTTCGGTCAT
    TTGAATAGAGAATTGAATTGTAAGATTCAAGTAGGTGGATCAGATCAATGGGGTAATATC
    ACAAGTGGTATTGAATTAATGCGTCGTATGTATGGTCAAACAGACGCATACGGTTTAACT
    ATTCCGCTTGTAACTAAATCAGATGGTAAGAAATTTGGTAAGTCTGAGTCAGGTGCTGTT
    TGGTTAGATGCTGAAAAAACAAGTCCTTATGAATTTTATCAATTCTGGATTAATCAATCA
    GACGAAGATGTAATTAAATTCTTAAAATACTTTACTTTCTTAGGAAAAGAAGAAATTGAT
    CGCTTAGAACAATCTAAAAATGAAGCACCGCATTTACGTGAAGCTCAAAAAACATTAGCT
    GAAGAAGTAACTAAATTTATTCATGGTGAAGATGCATTAAATGATGCAATCCGTATTTCA
    CAAGCATTATTTAGTGGTGATTTAAAATCATTATCAGCGAAAGAATTAAAAGATGGATTT
    AAAGATGTGCCTCAAGTGACATTATCAAATGACACAACAAATATCGTTGAAGTCCTTATT
    GAAACAGGCATTTCTCCTTCTAAACGACAAGCACGTGAAGATGTTAACAATGGTGCGATT
    TATATTAATGGTGAGAGACAACAAGATGTTAATTATGCTTTAGCACCAGAAGATAAAATT
    GATGGCGAATTTACGATTATTCGTCGCGGTAAGAAAAAATACTTCATGGTTAACTATCAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1263

⊟Protein[edit | edit source]

Protein Data Bank: 1JII
Protein Data Bank: 1JIJ
Protein Data Bank: 1JIK
Protein Data Bank: 1JIL

⊟General[edit | edit source]

  • locus tag: SA1550 [new locus tag: SA_RS08735 ]
  • symbol: TyrS
  • description: tyrosyl-tRNA synthetase
  • length: 420
  • theoretical pI: 5.05025
  • theoretical MW: 47597.2
  • GRAVY: -0.488333

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.1?  ExPASy
    Tyrosine--tRNA ligase ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation tyrosine--tRNA ligase (TIGR00234; EC 6.1.1.1; HMM-score: 446.5)
    and 2 more
    Genetic information processing Protein synthesis tRNA aminoacylation tryptophan--tRNA ligase (TIGR00233; EC 6.1.1.2; HMM-score: 13.7)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein uS4 (TIGR01017; HMM-score: 12.7)
  • TheSEED  :
    • Tyrosyl-tRNA synthetase (EC 6.1.1.1)
    tRNA synthetase relationships  Tyrosyl-tRNA synthetase (EC 6.1.1.1)
  • PFAM:
    HUP (CL0039) tRNA-synt_1b; tRNA synthetases class I (W and Y) (PF00579; HMM-score: 284.1)
    and 1 more
    S4 (CL0492) S4; S4 domain (PF01479; HMM-score: 34.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002263
    • TAT(Tat/SPI): 0.000233
    • LIPO(Sec/SPII): 0.000469
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTADSLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEGISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLEHGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYGLTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGKEEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAKELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNYALAPEDKIDGEFTIIRRGKKKYFMVNYQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA1517(citC)isocitrate dehydrogenase  [2] (data from MRSA252)
    SA1074(fabG)3-oxoacyl-ACP reductase  [2] (data from MRSA252)
    SA0943-1(pdhA)pyruvate dehydrogenase E1 component subunit alpha  [2] (data from MRSA252)
    SA0944(pdhB)pyruvate dehydrogenase E1 component subunit beta  [2] (data from MRSA252)
    SA0945(pdhC)branched-chain alpha-keto acid dehydrogenase E2 subunit  [2] (data from MRSA252)
    SA0946(pdhD)dihydrolipoamide dehydrogenase  [2] (data from MRSA252)
    SA1520(pykA)pyruvate kinase  [2] (data from MRSA252)
    SA0496(rplA)50S ribosomal protein L1  [2] (data from MRSA252)
    SA2035(rplE)50S ribosomal protein L5  [2] (data from MRSA252)
    SA2029(rplO)50S ribosomal protein L15  [2] (data from MRSA252)
    SA1084(rplS)50S ribosomal protein L19  [2] (data from MRSA252)
    SA1473(rplU)50S ribosomal protein L21  [2] (data from MRSA252)
    SA2041(rpsC)30S ribosomal protein S3  [2] (data from MRSA252)
    SA1245(sucA)2-oxoglutarate dehydrogenase E1  [2] (data from MRSA252)
    SA0506(tuf)elongation factor Tu  [2] (data from MRSA252)
    SA0802hypothetical protein  [2] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: T-box(Tyr) (transcription antitermination) regulon
    T-box(Tyr)(RNA)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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