PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_001850
• pan locus tag^?: SAUPAN004784000
• symbol: fumC
• pan gene symbol^?: fumC
• synonym:
• product: class II fumarate hydratase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_001850
• symbol: fumC
• product: class II fumarate hydratase
• replicon: chromosome
• strand: -
• coordinates: 1888769..1890154
• length: 1386
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  
  ATGTCAGTAAGAATTGAACATGATACTTTTGGAGAAATAGAAGTACCTGCAGATAAATAT
  TGGGGTGCTCAAACAGAAAGAAGTAAACGTAATTTCCCAGTTGGTAAAGAGCGTATGCCA
  ATCGAAGTAGTTTATGGTTTTGCACAACTAAAGCGTGCAGCAGCATTAGCTAATTTTGAT
  TTAGGAAAATTAAGCGAGGCAAAAAAAGATGCCATTGTATACGCATGTGATCAAATTTTA
  TCAGGTGAATTAGATGAACACTTCCCACTAGTTGTATGGCAAACAGGAAGCGGTACACAA
  AGTAATATGAATGTGAACGAAGTAGTAAGTTATGTTGCTAATATGTATTTAAAAGATCAT
  CAAAGTGATGAAAGTATCCACCCAAATGATGATGTAAATAAATCTCAAAGTTCGAATGAT
  ACATTCCCAACTGCTATGCACGTTGCATTATATCAAGAGGTTGAAACAAAATTAGAACCT
  GCATTAAAACTTTTAAGAAATACTTTGAAAGAAAAAGAAGATAAATTTGATTCAATTATT
  AAAATTGGTCGTACACATTTACAAGATGCAACGCCGATCAAACTAGGACAAGAGATTAGT
  GGCTGGCGTTATATGCTTGACCGTTGTGAAACAATGTTATCTGAATCTAAGAAGCACATT
  TTAAATCTTGCCATCGGTGGTACGGCTGTTGGTACTGGTATTAATGCGCATCCTGAATTT
  GGTGATAAAGTGGCACATTATATTTCAGAAAATACGGGTTATCCATTTGTATCTTCTGAA
  AATAAATTCCACGCACTTACAGCGCATGATGAAGTTGTTCAATTGCATGGAACATTGAAG
  GCATTAGCAGGAGACTTAATGAAAATTGCTAATGATGTGAGATGGTTGGCTTCAGGGCCA
  CGAGCTGGTTTGGCAGAAATTTCTATCCCTGAAAATGAACCAGGTTCATCAATTATGCCT
  GGTAAAGTTAATCCTACACAATGTGAAATGTTAACAATGGTTGCAGTCCAAGTAATGGGT
  AATGATACAGTTATTGGCTTCGCAAGTTCACAAGGTAACTTTGAATTGAATGTTTATAAA
  CCAGTTATTATGCATAATACACTACAATCAATTTACCTTTTAGCTGACGGTATGGAAACA
  TTTAATAACAATTGTGCAGTGGGCATTGAACCAATCGAAGAGAATATTGATAATTATTTA
  AATCAATCATTAATGTTAGTTACTGCATTAAATCCACATATTGGTTATGAAAAAGCTGCT
  CAAATTGCTAAGAAAGCCCATAAAGAAGGTTTAACTTTAAAAGAATCTGCAATTCAAACT
  GGATATGTTACAGAAGAACAATTTGAAGCATGGATTAAACCAGAAGATATGGTAGATCCT
  CATTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1386
  

⊟Protein

⊟General

• locus tag: JSNZ_001850
• symbol: FumC
• description: class II fumarate hydratase
• length: 461
• theoretical pI: 4.97681
• theoretical MW: 51121.6
• GRAVY: -0.330586

⊟Function

• reaction:
  EC 4.2.1.2?  ExPASy
  Fumarate hydratase (S)-malate = fumarate + H₂O
• TIGRFAM:
  Metabolism Energy metabolism TCA cycle fumarate hydratase, class II (TIGR00979; EC 4.2.1.2; HMM-score: 751.8)
  and 4 more
  Metabolism Energy metabolism Amino acids and amines aspartate ammonia-lyase (TIGR00839; EC 4.3.1.1; HMM-score: 470.4)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis adenylosuccinate lyase (TIGR00928; EC 4.3.2.2; HMM-score: 88.5)
  Metabolism Energy metabolism Other 3-carboxy-cis,cis-muconate cycloisomerase (TIGR02426; EC 5.5.1.2; HMM-score: 73.1)
  Metabolism Amino acid biosynthesis Glutamate family argininosuccinate lyase (TIGR00838; EC 4.3.2.1; HMM-score: 60.4)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  no clan defined Lyase_1; Lyase (PF00206; HMM-score: 378.8)
  and 5 more
  FumaraseC_C; Fumarase C C-terminus (PF10415; HMM-score: 90.5)
  DNA_primase_lrg (CL0242) DNA_primase_lrg; Eukaryotic and archaeal DNA primase, large subunit (PF04104; HMM-score: 17)
  no clan defined Rubis-subs-bind; Rubisco LSMT substrate-binding (PF09273; HMM-score: 14.9)
  P-loop_NTPase (CL0023) G-alpha; G-protein alpha subunit (PF00503; HMM-score: 13.9)
  KNTase_C (CL0291) NTase_sub_bind; Nucleotidyltransferase substrate binding protein like (PF08780; HMM-score: 12.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9369
  • Cytoplasmic Membrane Score: 0.0009
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0622
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003911
  • TAT(Tat/SPI): 0.000952
  • LIPO(Sec/SPII): 0.000795
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVVYGFAQLKRAAALANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSNMNVNEVVSYVANMYLKDHQSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEPALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKKHILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLHGTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMVAVQVMGNDTVIGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPIEENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEAWIKPEDMVDPH

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications